LOCUS       sequence-167416-         854 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-491-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    138..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(175..194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(594..615)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatcgatcac gagactagcc tcgagagatc tgatcataat cagccatacc acatttgtag
       61 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      121 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaggcaata
      181 gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt ggtttgtcca
      241 aactcatcaa tgtatcttat catgtctgga tctcaaatcc ctcggaagct gcgcctgtca
      301 tcgaattcct gcagcccggt gcatgactaa gctagtaccg gtagtgggga acccttccat
      361 gaggagtggg gaacccttcc atgaggagtg gggaaccctt ccatgaggag tggggaaccc
      421 ttccatgagg agtggggaac ccttccatga ggagtgggga acccttccat gagggctacg
      481 tcgaccagta ctccccagtt aaggtcggct agctcagtta gcctccccca tctctcgacg
      541 cggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      601 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      661 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      721 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      781 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      841 tccagcttgt gccc
//
