LOCUS       sequence-167436-        6099 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing PIK3CG , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CG_p.W212C
FEATURES             Location/Qualifiers
     source          1..6099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3098..3109
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3499..3598)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3615..3634)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3616..3634)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3639..3655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3639..3655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4086..4877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4970..5558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5459..5478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5712..5729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5888..5915)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6007..6093)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggggctg gagaactata aacagcccgt ggtgctgaga gaggacaact
      241 gccgaaggcg ccggaggatg aagccgcgca gtgctgcggc cagcctgtcc tccatggagc
      301 tcatccccat cgagttcgtg ctgcccacca gccagcgcaa atgcaagagc cccgaaacgg
      361 cgctgctgca cgtggccggc cacggcaacg tggagcagat gaaggcccag gtgtggctgc
      421 gagcgctgga gaccagcgtg gcggcggact tctaccaccg gctgggaccg catcacttcc
      481 tcctgctcta tcagaagaag gggcagtggt acgagatcta cgacaagtac caggtggtgc
      541 agactctgga ctgcctgcgc tactggaagg ccacgcaccg gagcccgggc cagatccacc
      601 tggtgcagcg gcacccgccc tccgaggagt cccaagcctt ccagcggcag ctcacggcgc
      661 tgattggcta tgacgtcact gacgtcagca acgtgcacga cgatgagctg gagttcacgc
      721 gccgtggctt ggtgaccccg cgcatggcgg aggtggccag ccgcgacccc aagctctacg
      781 ccatgcaccc gtgggtgacg tccaagcccc tcccggagta cctgtgtaag aagattgcca
      841 acaactgcat cttcatcgtc attcaccgca gcaccaccag ccagaccatt aaggtctcac
      901 ccgacgacac ccccggcgcc atcctgcaga gcttcttcac caagatggcc aagaagaaat
      961 ctctgatgga tattcccgaa agccaaagcg aacaggattt tgtgctgcgc gtctgtggcc
     1021 gggatgagta cctggtgggc gaaacgccca tcaaaaactt ccagtgggtg aggcactgcc
     1081 tcaagaacgg agaagagatt cacgtggtac tggacacgcc tccagacccg gccctagacg
     1141 aggtgaggaa ggaagagtgg ccgctggtgg acgactgcac gggagtcacc ggctaccatg
     1201 agcagcttac catccacggc aaggaccacg agagtgtgtt caccgtgtcc ctgtgggact
     1261 gcgaccgcaa gttcagggtc aagatcagag gcattgatat ccccgtcctg cctcggaaca
     1321 ccgacctcac agtttttgta gaggcaaaca tccagcatgg gcaacaagtc ctttgccaaa
     1381 ggagaaccag ccccaaaccc ttcacagagg aggtgctgtg gaatgtgtgg cttgagttca
     1441 gtatcaaaat caaagacttg cccaaagggg ctctactgaa cctccagatc tactgcggta
     1501 aagctccagc actgtccagc aaggcctctg cagagtcccc cagttctgag tccaagggca
     1561 aagttcagct tctctattat gtgaacctgc tgctgataga ccaccgtttc ctcctgcgcc
     1621 gtggagaata cgtcctccac atgtggcaga tatctgggaa gggagaagac caaggaagct
     1681 tcaatgctga caaactcacg tctgcaacta acccagacaa ggagaactca atgtccatct
     1741 ccattcttct ggacaattac tgccacccga tagccctgcc taagcatcag cccacccctg
     1801 acccggaagg ggaccgggtt cgagcagaaa tgcccaacca gcttcgcaag caattggagg
     1861 cgatcatagc cactgatcca cttaaccctc tcacagcaga ggacaaagaa ttgctctggc
     1921 attttagata cgaaagcctt aagcacccaa aagcatatcc taagctattt agttcagtga
     1981 aatggggaca gcaagaaatt gtggccaaaa cataccaatt gttggccaga agggaagtct
     2041 gggatcaaag tgctttggat gttgggttaa caatgcagct cctggactgc aacttctcag
     2101 atgaaaatgt aagagccatt gcagttcaga aactggagag cttggaggac gatgatgttc
     2161 tgcattacct tctacaattg gtccaggctg tgaaatttga accataccat gatagtgccc
     2221 ttgccagatt tctgctgaag cgtggtttaa gaaacaaaag aattggtcac tttttgtttt
     2281 ggttcttgag aagtgagata gcccagtcca gacactatca gcagaggttc gctgtgattc
     2341 tggaagccta tctgaggggc tgtggcacag ccatgctgca cgactttacc caacaagtcc
     2401 aagtaatcga gatgttacaa aaagtcaccc ttgatattaa atcgctctct gctgaaaagt
     2461 atgacgtcag ttcccaagtt atttcacaac ttaaacaaaa gcttgaaaac ctgcagaatt
     2521 ctcaactccc cgaaagcttt agagttccat atgatcctgg actgaaagca ggagcgctgg
     2581 caattgaaaa atgtaaagta atggcctcca agaaaaaacc actatggctt gagtttaaat
     2641 gtgccgatcc tacagcccta tcaaatgaaa caattggaat tatctttaaa catggtgatg
     2701 atctgcgcca agacatgctt attttacaga ttctacgaat catggagtct atttgggaga
     2761 ctgaatcttt ggatctatgc ctcctgccat atggttgcat ttcaactggt gacaaaatag
     2821 gaatgatcga gattgtgaaa gacgccacga caattgccaa aattcagcaa agcacagtgg
     2881 gcaacacggg agcatttaaa gatgaagtcc tgaatcactg gctcaaagaa aaatccccta
     2941 ctgaagaaaa gtttcaggca gcagtggaga gatttgttta ttcctgtgca ggctactgtg
     3001 tggcaacctt tgttcttgga ataggcgaca gacacaatga caatattatg atcaccgaga
     3061 caggaaacct atttcatatt gacttcgggc acattcttgg gaattacaaa agtttcctgg
     3121 gcattaataa agagagagtg ccatttgtgc taacccctga cttcctcttt gtgatgggaa
     3181 cttctggaaa gaagacaagc ccacacttcc agaaatttca ggacatctgt gttaaggctt
     3241 atctagccct tcgtcatcac acaaacctac tgatcatcct gttctccatg atgctgatga
     3301 caggaatgcc ccagttaaca agcaaagaag acattgaata tatccgggat gccctcacag
     3361 tggggaaaaa tgaggaggat gctaaaaagt attttcttga tcagatcgaa gtttgcagag
     3421 acaaaggatg gactgtgcag tttaattggt ttctacatct tgttcttggc atcaaacaag
     3481 gagagaaaca ttcagcctac ccaactttct tgtacaaagt tggcattata agaaagcatt
     3541 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     3601 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     3661 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     3721 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     3781 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     3841 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     3901 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     3961 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     4021 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     4081 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     4141 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     4201 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     4261 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     4321 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     4381 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     4441 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     4501 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     4561 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     4621 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     4681 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     4741 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     4801 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     4861 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     4921 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4981 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5041 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5101 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5161 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5221 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5281 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5341 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5401 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5461 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5521 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5581 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5641 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5701 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5761 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5821 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     5881 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     5941 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     6001 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     6061 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
