LOCUS       sequence-167567-        5108 bp    DNA     circular SYN 15-APR-2025
DEFINITION  DsREDmonomer-based BiFC vector for plants (Positive control: MXMT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBRN168-MXMT
FEATURES             Location/Qualifiers
     source          1..5108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..365
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     308..328
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    385..440
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     858..877
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2126..2378
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2416..2432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2451..2467)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2464..2486)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2475..2491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2499..2505,2506..2523,2524..2529))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2544..2565
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2682..2699)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2853..3441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2933..2952)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3612..4403,4404..4472))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4235..4254
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4473..4577)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4645..4663
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4701..4723)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4823..4842
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5036..5058
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5050..5067
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5051..5067
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcagaagac cgagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
       61 ggattccatt gcccagctat ctgtcactta tcgaaaggac agtagaaaag gaaggtggct
      121 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      181 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      241 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      301 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      361 ggacaggctt cttgagatcc ttcaacaatt accaacaaca acaaacaaca aacaacatta
      421 caattactat ttacaattac agtcgactct agaggatcca tggacaacac cgaggacgtc
      481 atcaaggagt tcatgcagtt caaggtgcgc atggagggct ccgtgaacgg ccactacttc
      541 gagatcgagg gcgagggcga gggcaagccc tacgagggca cccagaccgc caagctgcag
      601 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac
      661 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacatgaa gctgtccttc
      721 cccgagggct tcacctggga gcgctccatg aacttcgagg acggcggcgt ggtggaggtg
      781 cagcaggact cctccctgca ggacggcacc ttcatctaca aggtgaagtt caagggcgtg
      841 aacttccccg ccgacggccc cgtaatgcag aagaagactg ccggctggga gccctccacc
      901 gagaagctgt acccccagga cggcgtgctg aagggcgaga tctcccacgc cctgaagctg
      961 aagctgtaca gatggagctc caagaagtcc tgcatatgaa tgaaggtgaa ggcgatacaa
     1021 gctacgccaa gaatgcatcc tacaatctgg ctcttgccaa ggtgaaacct ttccttgaac
     1081 aatgcatacg agaattgttg cgggccaact tgcccaacat caacaagtgc attaaagttg
     1141 cggatttggg atgcgcttct ggaccaaaca cacttttaac agtgcgggac attgtgcaaa
     1201 gtattgacaa agttggccag gaagagaaga atgaattaga acgtcccacc attcagattt
     1261 ttctgaatga tcttttccaa aatgatttca attcggtttt caagttgctg ccaagcttct
     1321 accgcaaact cgagaaagaa aatggacgca agataggatc gtgcctaata agcgcaatgc
     1381 ctggctcttt ctacggcaga ctcttccccg aggagtccat gcattttttg cactcttgtt
     1441 acagtgttca ttggttatct caggttccca gcggtttggt gattgaattg gggattggtg
     1501 caaacaaagg gagtatttac tcttccaaag gatgtcgtcc gcccgtccag aaggcatatt
     1561 tggatcaatt tacgaaagat tttaccacat ttctaaggat tcattcgaaa gagttgtttt
     1621 cacgtggccg aatgctcctt acctgcattt gtaaagtaga tgaattcgac gaaccgaatc
     1681 ccttagactt acttgacatg gcaataaacg acttgattgt tgagggactt ctggaggaag
     1741 aaaaattgga tagtttcaat attccattct ttacacctcc agcagaagaa gtaaagtgca
     1801 tagttgagga ggaaggttct tgcgaaattt tatatctgga gacttttaag gcccattatg
     1861 atgctgcctt ctctattgat gatgattacc cagtaagatc ccatgaacaa attaaagcag
     1921 agtatgtggc atcattaatt agatcagttt acgaacccat cctcgcaagt cattttggag
     1981 aagctattat gcctgactta ttccacaggc ttgcgaagca tgcagcaaag gttctccaca
     2041 tgggcaaagg ctgctataat aatcttatca tttctctcgc caaaaagcca gagaagtcag
     2101 acgtgtaagc ggccgcccgg ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag
     2161 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     2221 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     2281 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     2341 taaattatcg cgcgcggtgt catctatgtt actagatccg atgataagct gtcaaacata
     2401 gaattcgtaa tcatggtcat agctgtttcc tgtgtgaatt cgtaatcatg gtcatagctg
     2461 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2521 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     2581 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     2641 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2701 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2761 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2821 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2881 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2941 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3001 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3061 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3121 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3181 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3241 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3301 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3361 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3421 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3481 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3541 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3601 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3661 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3721 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3781 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3841 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3901 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3961 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4021 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4081 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4141 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4201 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4261 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4321 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4381 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4441 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4501 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4561 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4621 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     4681 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4741 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4801 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     4861 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     4921 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     4981 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     5041 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgatctagag
     5101 gatccccc
//
