LOCUS       sequence-167593-       11276 bp    DNA     circular SYN 15-APR-2025
DEFINITION  target  miRNA832-3p  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM832-3p
FEATURES             Location/Qualifiers
     source          1..11276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2423..2803
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2809..3360
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3370..3622
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3708..3732
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4157..4951
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5038..5626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5780..5797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5812..5952
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5878..5897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6038..6060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6296..6490
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6556..7629)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8058..8687)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9988..10012
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10200..10222
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10214..10231
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10215..10231
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10256..10963)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11190..11209
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11190..11208
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca cagacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc cctatataag gaagttcatt tcatttggag aggacagccc aagcttcttg
      361 cttggatcta tgggaatcaa gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatct tgcttggatc tatgggaatc aagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatattg
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa acctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacacgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaag aaaggcggac aggtatccgg taatctgctg cttgcaaact taattaagaa
     2341 aggcggacag gtatccggta atctgctgct tgcaaactta attaactctc tagactcacc
     2401 taggatccat gaattctgag atttttcaaa tcagtgcgca agacgtgacg taagtatccg
     2461 agtcagtttt tatttttcta ctaatttggt cgtttatttc ggcgtgtagg acatggcaac
     2521 cgggcctgaa tttcgcgggt attctgtttc tattccaact ttttcttgat ccgcagccat
     2581 taacgacttt tgaatagata cgctgacacg ccaagcctcg ctagtcaaaa gtgtaccaaa
     2641 caacgcttta cagcaagaac ggaatgcgcg tgacgctcgc ggtgacgcca tttcgccttt
     2701 tcagaaatgg ataaatagcc ttgcttccta ttatatcttc ccaaattacc aatacattac
     2761 actagcatct gaatttcata accaatctcg atacaccaaa tcgaatcgat gagcccagaa
     2821 cgacgcccgg ccgacatccg ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc
     2881 gtcaaccact acatcgagac aagcacggtc aacttccgta ccgagccgca ggaaccgcag
     2941 gagtggacgg acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg
     3001 gacggcgagg tcgccggcat cgcctacgcg ggcccctgga aggcacgcaa cgcctacgac
     3061 tggacggccg agtcgaccgt gtacgtctcc ccccgccacc agcggacggg actgggctcc
     3121 acgctctaca cccacctgct gaagtccctg gaggcacagg gcttcaagag cgtggtcgct
     3181 gtcatcgggc tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc
     3241 cgcggcatgc tgcgggcggc cggcttcaag cacgggaact ggcatgacgt gggtttctgg
     3301 cagctggact tcagcctgcc ggtaccgccc cgtccggtcc tgcccgtcac cgagatttga
     3361 ctgcagaatc ttggactccc atgttggcaa aggcaaccaa acaaacaatg aatgatccgc
     3421 tcctgcatat ggggcggttt gagtatttca actgccattt gggctgaatt gaagacatgc
     3481 tcctgtcaga aattccgtga tcttactcaa tattcagtaa tctcggccaa tatcctaaat
     3541 gtgcgtggct ttatctgtct ttgtattgtt tcatcaattc atgtaacgtt tgcttttctt
     3601 atgaattttc aaataaatta tcagatccga gctcgaatta attcggcgtt aattcagtac
     3661 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     3721 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     3781 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     3841 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     3901 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     3961 cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg
     4021 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
     4081 aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag
     4141 aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga
     4201 aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt
     4261 gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta
     4321 tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa
     4381 ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg
     4441 cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta
     4501 tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag
     4561 cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga
     4621 ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt
     4681 aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa
     4741 catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc
     4801 ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga
     4861 acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa
     4921 atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta
     4981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5161 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5701 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5761 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     5821 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     5881 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     5941 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     6001 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     6061 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     6121 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     6181 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     6241 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     6301 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     6361 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     6421 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     6481 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     6541 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     6601 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     6661 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     6721 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     6781 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     6841 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     6901 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     6961 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     7021 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     7081 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     7141 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     7201 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     7261 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     7321 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     7381 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     7441 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     7501 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     7561 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     7621 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcaggca
     7681 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     7741 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     7801 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     7861 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     7921 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     7981 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     8041 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     8101 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     8161 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
     8221 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
     8281 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
     8341 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
     8401 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
     8461 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
     8521 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
     8581 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
     8641 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
     8701 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
     8761 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
     8821 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
     8881 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
     8941 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
     9001 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
     9061 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
     9121 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
     9181 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
     9241 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
     9301 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
     9361 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
     9421 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
     9481 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
     9541 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
     9601 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
     9661 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
     9721 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
     9781 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
     9841 actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg
     9901 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
     9961 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
    10021 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc
    10081 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10141 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10201 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcctgct
    10261 gagcctcgac atgttgtcgc aaaattcgcc ctggacccgc ccaacgattt gtcgtcactg
    10321 tcaaggtttg acctgcactt catttggggc ccacatacac caaaaaaatg ctgcataatt
    10381 ctcggggcag caagtcggtt acccggccgc cgtgctggac cgggttgaat ggtgcccgta
    10441 actttcggta gagcggacgg ccaatactca acttcaagga atctcaccca tgcgcgccgg
    10501 cggggaaccg gagttccctt cagtgagcgt tattagttcg ccgctcggtg tgtcgtagat
    10561 actagcccct ggggcacttt tgaaatttga ataagattta tgtaatcagt cttttaggtt
    10621 tgaccggttc tgccgctttt tttaaaattg gatttgtaat aataaaacgc aattgtttgt
    10681 tattgtggcg ctctatcata gatgtcgcta taaacctatt cagcacaata tattgttttc
    10741 attttaatat tgtacatata agtagtaggg tacaatcagt aaattgaacg gagaatatta
    10801 ttcataaaaa tacgatagta acgggtgata tattcattag aatgaaccga aaccggcggt
    10861 aaggatctga gctacacatg ctcaggtttt ttacaacgtg cacaacagaa ttgaaagcaa
    10921 atatcatgcg atcataggcg tctcgcatat ctcattaaag caggactcta gggactagtc
    10981 ccgggtctta attaagaaag gcggacaggt atccggtaat ctgctgcttg caaacttaat
    11041 taagtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaaggaga tcctttggat
    11101 cacaaaaaaa aagcccgctc gttaggcggg ttaaattccc gggtactgca gccgattcat
    11161 taatgcaggt taacctggct tatcgaaatt aatacgactc actataggga gaccggcaga
    11221 tctgatccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctat
//
