LOCUS       sequence-16763-e        1377 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4052
FEATURES             Location/Qualifiers
     source          1..1377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             251..271
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             293..313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             335..355
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             377..397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(440..468)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngttactga tcttctaact aactattctc atttaaattt tcagagctta aaaatggctg
       61 aaatcactca caacgatgga tacgctaaca acttggaaat gaaataagct tgcatgcctg
      121 caggtcgact ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
      181 atactaacat aacatagaac attttcagga ggacccttgg agggtaccga gctcagaaaa
      241 aatgactgct ccaaagaaga agcgtaaggt accggcagaa aaaatgactg ctccaaagaa
      301 gaagcgtaag gtaccggcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggc
      361 agaaaaaatg actgctccaa agaagaagcg taaggtaccg gtagaaaaaa tgagtaaagg
      421 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      481 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      541 taaatttatt tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat
      601 actaactaac cctgattatt taaattttca gccaacactt gtcactactt tctgttatgg
      661 tgttcaatgc ttctcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc
      721 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      781 gacacgtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtgc
      841 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      901 ttaaagaaga tggaaacntt cttggacnca aattggaata caactataac tcacacaatg
      961 tatacctcat ggcagacaac caaagaatgg aatcaangtt gtaagtttaa cntgatttnc
     1021 tacctactan tctgatttaa ttttcnaact tccaanttaa cccaccttga agatggagcg
     1081 ttcactaccn accttttcac aaatntccat ggggaggcct gtcntttaca anaccttnct
     1141 gtccnccatn tgccnttcaa aatccncnaa annaacccnt gncctttngn ttnaaccccg
     1201 cggnttncct gggtgganan tnacaaaccc canntngatc cncccgcncn anaancggna
     1261 ccccccnccn aattcantta acgaatgttt tncnaaantn tncccgggtt tcgccnaaaa
     1321 aacccaaaat ttttnccccc cccncccccc cnaaggnant ttttcntttt ngggngg
//
