LOCUS       sequence-167685-         862 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  FRET biosensor. Visualization of cytosolic FAK activity in live 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Seong J, Ouyang M, Kim T, Sun J, Wen PC, Lu S, Zhuo Y, Llewellyn NM,
            Schlaepfer DD, Guan JL, Chien S, Wang Y
  TITLE     Detection of focal adhesion kinase activation at membrane 
            microdomains by fluorescence resonance energy transfer.
  JOURNAL   Nat Commun. 2011 Jul 26;2:406. doi: 10.1038/ncomms1414.
  PUBMED    21792185
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-07-26"; volume: "2:406. doi"; pages: " 
            10.1038/ncomms1414"
COMMENT     Sequence Label: Cytosolic FAK biosensor
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(456..477)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     805..824
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcaccatcta attcaaccaa aattgggaca acaccagtga ataattcttc acctttagag
       61 agctcctcgt cgatgatctc agcgtagtcg tcggtctcag aaccctcacc agaacccggc
      121 ttcccagatc cagatgtaga cccacagacg ttagtcaggc ggtggcacaa gccatcagca
      181 tgtttggagt agtaggccac cagctgctgc aggctgctga actgtgtgcg tgaggtgatg
      241 tagaagccgc cgctgtccag cttgcggatc ttgtagtgct tcacattgag ccccttggcg
      301 ttgtcaaagt cagaaacgga gaggcaatag gcaccttttg tcgtctcgct ctcccggacc
      361 aagaaggttc cccgggggtt ttcggggttg agcagcagcc gctcggactc ccgacgagtg
      421 atcttcccaa aataccaatg catgcgggcg gcggtcacga actccagcag gaccatgtga
      481 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
      541 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
      601 acgctgccgt cctcgatgtt gtggcggatc ttgaagtggg ccttgatgcc gttcttctgc
      661 ttgtcggcgg tgatatagac gttgtggctg atgtagttgt actccagctt gtgccccagg
      721 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
      781 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggta
      841 cgctcctgga cgtagccttc gg
//
