LOCUS       sequence-167904-         909 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Strain: AM5116, Used for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Kim YI, Vinyard DJ, Ananyev GM, Dismukes GC, Golden SS
  TITLE     Oxidized quinones signal onset of darkness directly to the 
            cyanobacterial circadian oscillator.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17765-9. doi: 
            10.1073/pnas.1216401109. Epub 2012 Oct 15.
  PUBMED    23071342
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216401109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-10-30- 30"; volume: "109"; issue: "44"; pages: "17765-9"
COMMENT     Sequence Label: pET28-SUMO-KaiB
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(175..630)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(312..333)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     524..543
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(667..714)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     710..728
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(781..798)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
       61 tgatgctcga tgagtttttc taagaattaa ttcatgagcg gatacatatt tgaatgtatt
      121 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
      181 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
      241 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
      301 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
      361 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
      421 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
      481 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
      541 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
      601 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc aatccggata tagttcctcc
      661 tttcagcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctagttattg
      721 ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatctca
      781 gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttt tagaagtcgt cggaatcttg
      841 aagttcgccg tagagtaaat caaggccaat caaaactttc tcacggtcgg ataaatcacc
      901 aataatccg
//
