LOCUS       sequence-167905-        3917 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing ACTG1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ACTG1_p.K61E
FEATURES             Location/Qualifiers
     source          1..3917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1317..1416)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1433..1452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1434..1452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1457..1473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1457..1473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1904..2695
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2788..3376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3277..3296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3530..3547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3706..3733)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3825..3911)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggaagaa gagatcgccg cgctggtcat tgacaatggc tccggcatgt
      241 gcaaagctgg ttttgctggg gacgacgctc cccgagccgt gtttccttcc atcgtcgggc
      301 gccccagaca ccagggcgtc atggtgggca tgggccagaa ggactcctac gtgggcgacg
      361 aggcccagag cgagcgtggc atcctgaccc tgaagtaccc cattgagcat ggcatcgtca
      421 ccaactggga cgacatggag aagatctggc actacacctt ctacaacgag ctgcgcgtgg
      481 ccccggagga gcacccagtg ctgctgaccg aggcccccct gaaccccaag gccaacagag
      541 agaagatgac tcagattatg tttgagacct tcaacacccc ggccatgtac gtggccatcc
      601 aggccgtgct gtccctctac gcctctgggc gcaccactgg cattgtcatg gactctggag
      661 acggggtcac ccacacggtg cccatctacg agggctacgc cctcccccac gccatcctgc
      721 gtctggacct ggctggccgg gacctgaccg actacctcat gaagatcctc actgagcgag
      781 gctacagctt caccaccacg gccgagcggg aaatcgtgcg cgacatcaag gagaagctgt
      841 gctacgtcgc cctggacttc gagcaggaga tggccaccgc cgcatcctcc tcttctctgg
      901 agaagagcta cgagctgccc gatggccagg tcatcaccat tggcaatgag cggttccggt
      961 gtccggaggc gctgttccag ccttccttcc tgggtatgga atcttgcggc atccacgaga
     1021 ccaccttcaa ctccatcatg aagtgtgacg tggacatccg caaagacctg tacgccaaca
     1081 cggtgctgtc gggcggcacc accatgtacc cgggcattgc cgacaggatg cagaaggaga
     1141 tcaccgccct ggcgcccagc accatgaaga tcaagatcat cgcaccccca gagcgcaagt
     1201 actcggtgtg gatcggtggc tccatcctgg cctcactgtc caccttccag cagatgtgga
     1261 ttagcaagca ggagtacgac gagtcgggcc cctccatcgt ccaccgcaaa tcttctaccc
     1321 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1381 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1441 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1501 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     1561 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     1621 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     1681 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     1741 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     1801 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     1861 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     1921 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     1981 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2041 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2101 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2161 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2221 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2281 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     2341 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     2401 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     2461 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     2521 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     2581 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     2641 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     2701 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     2761 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2821 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2881 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2941 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3001 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3061 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3121 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3181 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3241 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3301 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3361 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3421 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3481 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3541 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3601 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3661 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
     3721 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     3781 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     3841 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
     3901 cgttttattt gatgcct
//
