LOCUS       sequence-167912-        4119 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing EDNRB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_EDNRB_p.L349I
FEATURES             Location/Qualifiers
     source          1..4119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1519..1618)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1635..1654)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1636..1654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1659..1675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1659..1675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2106..2897
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGSLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2990..3578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3479..3498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3732..3749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3908..3935)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4027..4113)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcagccgcct ccaagtctgt gcggacgcgc cctggttgcg ctggttcttg
      241 cctgcggcct gtcgcggatc tggggagagg agagaggctt cccgcctgac agggccactc
      301 cgcttttgca aaccgcagag ataatgacgc cacccactaa gaccttatgg cccaagggtt
      361 ccaacgccag tctggcgcgg tcgttggcac ctgcggaggt gcctaaagga gacaggacgg
      421 caggatctcc gccacgcacc atctcccctc ccccgtgcca aggacccatc gagatcaagg
      481 agactttcaa atacatcaac acggttgtgt cctgccttgt gttcgtgctg gggatcatcg
      541 ggaactccac acttctgaga attatctaca agaacaagtg catgcgaaac ggtcccaata
      601 tcttgatcgc cagcttggct ctgggagacc tgctgcacat cgtcattgac atccctatca
      661 atgtctacaa gctgctggca gaggactggc catttggagc tgagatgtgt aagctggtgc
      721 ctttcataca gaaagcctcc gtgggaatca ctgtgctgag tctatgtgct ctgagtattg
      781 acagatatcg agctgttgct tcttggagta gaattaaagg aattggggtt ccaaaatgga
      841 cagcagtaga aattgttttg atttgggtgg tctctgtggt tctggctgtc cctgaagcca
      901 taggttttga tataattacg atggactaca aaggaagtta tctgcgaatc tgcttgcttc
      961 atcccgttca gaagacagct ttcatgcagt tttacaagac agcaaaagat tggtggctgt
     1021 tcagtttcta tttctgcttg ccattggcca tcactgcatt tttttataca ctaatgacct
     1081 gtgaaatgtt gagaaagaaa agtggcatgc agattgcttt aaatgatcac ctaaagcaga
     1141 gacgggaagt ggccaaaacc gtcttttgcc tggtccttgt ctttgccctc tgctggcttc
     1201 cccttcacct cagcaggatt ctgaagctca ctatttataa tcagaatgat cccaatagat
     1261 gtgaactttt gagctttctg ttggtattgg actatattgg tatcaacatg gcttcactga
     1321 attcctgcat taacccaatt gctctgtatt tggtgagcaa aagattcaaa aactgcttta
     1381 agtcatgctt atgctgctgg tgccagtcat ttgaagaaaa acagtccttg gaggaaaagc
     1441 agtcgtgctt aaagttcaaa gctaatgatc acggatatga caacttccgt tccagtaata
     1501 aatacagctc atcttgattg ccaactttct tgtacaaagt tggcattata agaaagcatt
     1561 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     1621 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1681 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1741 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1801 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1861 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1921 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1981 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2041 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2101 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2161 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2221 atggcagcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2281 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2341 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2401 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2461 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2521 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2581 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2641 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2701 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2761 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2821 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2881 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2941 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3781 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3841 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3901 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3961 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4021 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4081 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
