LOCUS       sequence-167922-        3974 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing MAP2K1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP2K1_p.L177M
FEATURES             Location/Qualifiers
     source          1..3974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      15..603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     504..523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     757..774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(933..960)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1052..1138)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1187..1209
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1201..1218
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1202..1218
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1234..1333
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2518..2617)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2634..2653)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2635..2653)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2658..2674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2658..2674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3105..3896
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
       61 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      781 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      841 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
      901 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
      961 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     1021 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     1081 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     1141 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     1201 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
     1261 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
     1321 aaaaaagttg gcaccatgcc caagaagaag ccgacgccca tccagctgaa cccggccccc
     1381 gacggctctg cagttaacgg gaccagctct gcggagacca acttggaggc cttgcagaag
     1441 aagctggagg agctagagct tgatgagcag cagcgaaagc gccttgaggc ctttcttacc
     1501 cagaagcaga aggtgggaga actgaaggat gacgactttg agaagatcag tgagctgggg
     1561 gctggcaatg gcggtgtggt gttcaaggtc tcccacaagc cttctggcct ggtcatggcc
     1621 agaaagctaa ttcatctgga gatcaaaccc gcaatccgga accagatcat aagggagctg
     1681 caggttctgc atgagtgcaa ctctccgtac atcgtgggct tctatggtgc gttctacagc
     1741 gatggcgaga tcagtatctg catggagcac atggatggag gttctctgga tcaagtcctg
     1801 aagaaagctg gaagaattcc tgaacaaatt ttaggaaaag ttagcattgc tgtaataaaa
     1861 ggcatgacat atctgaggga gaagcacaag atcatgcaca gagatgtcaa gccctccaac
     1921 atcctagtca actcccgtgg ggagatcaag ctctgtgact ttggggtcag cgggcagctc
     1981 atcgactcca tggccaactc cttcgtgggc acaaggtcct acatgtcgcc agaaagactc
     2041 caggggactc attactctgt gcagtcagac atctggagca tgggactgtc tctggtagag
     2101 atggcggttg ggaggtatcc catccctcct ccagatgcca aggagctgga gctgatgttt
     2161 gggtgccagg tggaaggaga tgcggctgag accccaccca ggccaaggac ccccgggagg
     2221 ccccttagct catacggaat ggacagccga cctcccatgg caatttttga gttgttggat
     2281 tacatagtca acgagcctcc tccaaaactg cccagtggag tgttcagtct ggaatttcaa
     2341 gattttgtga ataaatgctt aataaaaaac cccgcagaga gagcagattt gaagcaactc
     2401 atggttcatg cttttatcaa gagatctgat gctgaggaag tggattttgc aggttggctc
     2461 tgctccacca tcggccttaa ccagcccagc acaccaaccc atgctgctgg cgttgattgc
     2521 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     2581 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2641 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2701 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     2761 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2821 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2881 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2941 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     3001 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     3061 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     3121 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     3181 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3241 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3301 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3361 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3421 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3481 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3541 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3601 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3661 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3721 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3781 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3841 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3901 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3961 accccgtaga aaag
//
