LOCUS       sequence-167967-        4038 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing IDH1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IDH1_p.R132L
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             338..1129
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     LELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1222..1810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1711..1730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1964..1981
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2140..2167)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2259..2345)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2394..2416
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2408..2425
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2409..2425
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2441..2540
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3789..3888)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3905..3924)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3906..3924)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3929..3945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3929..3945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
       61 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
      121 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      181 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      241 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      301 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
      361 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      421 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      481 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      541 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      601 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc tcgaactgca
      661 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      721 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      781 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      841 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      901 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
      961 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1021 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1081 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     1141 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     1201 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1261 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1321 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1381 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1441 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1501 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1561 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1621 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1681 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1741 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1801 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1861 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1921 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1981 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2041 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2101 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     2161 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     2221 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     2281 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     2341 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
     2401 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg
     2461 actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac
     2521 tttgtacaaa aaagttggca ccatgtccaa aaaaatcagt ggcggttctg tggtagagat
     2581 gcaaggagat gaaatgacac gaatcatttg ggaattgatt aaagagaaac tcatttttcc
     2641 ctacgtggaa ttggatctac atagctatga tttaggcata gagaatcgtg atgccaccaa
     2701 cgaccaagtc accaaggatg ctgcagaagc tataaagaag cataatgttg gcgtcaaatg
     2761 tgccactatc actcctgatg agaagagggt tgaggagttc aagttgaaac aaatgtggaa
     2821 atcaccaaat ggcaccatac gaaatattct gggtggcacg gtcttcagag aagccattat
     2881 ctgcaaaaat atcccccggc ttgtgagtgg atgggtaaaa cctatcatca taggtcttca
     2941 tgcttatggg gatcaataca gagcaactga ttttgttgtt cctgggcctg gaaaagtaga
     3001 gataacctac acaccaagtg acggaaccca aaaggtgaca tacctggtac ataactttga
     3061 agaaggtggt ggtgttgcca tggggatgta taatcaagat aagtcaattg aagattttgc
     3121 acacagttcc ttccaaatgg ctctgtctaa gggttggcct ttgtatctga gcaccaaaaa
     3181 cactattctg aagaaatatg atgggcgttt taaagacatc tttcaggaga tatatgacaa
     3241 gcagtacaag tcccagtttg aagctcaaaa gatctggtat gagcataggc tcatcgacga
     3301 catggtggcc caagctatga aatcagaggg aggcttcatc tgggcctgta aaaactatga
     3361 tggtgacgtg cagtcggact ctgtggccca agggtatggc tctctcggca tgatgaccag
     3421 cgtgctggtt tgtccagatg gcaagacagt agaagcagag gctgcccacg ggactgtaac
     3481 ccgtcactac cgcatgtacc agaaaggaca ggagacgtcc accaatccca ttgcttccat
     3541 ttttgcctgg accagagggt tagcccacag agcaaagctt gataacaata aagagcttgc
     3601 cttctttgca aatgctttgg aagaagtctc tattgagaca attgaggctg gcttcatgac
     3661 caaggacttg gctgcttgca ttaaaggttt acccaatgtg caacgttctg actacttgaa
     3721 tacatttgag ttcatggata aacttggaga aaacttgaag atcaaactag ctcaggccaa
     3781 actttaattg ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     3841 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     3901 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     3961 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     4021 gaccagccag gacagaaa
//
