LOCUS       sequence-167984-         999 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 
            selection. GFP was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and smu-1 introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1415
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(908..928)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(931..955)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtagttattg tagttattag attcaaaaaa agtaattgta taaatgtttt ggtccaaaat
       61 ttacaagttt tgtgaatttt ctagttgaaa attgaaaatt tgaaaattac atttaaaaaa
      121 ttgtgtattt ctcaaaactt ttttgtatga aattttttct caaataatga cttttggggc
      181 taaaaagcat gaaaattcac aattttaagg gaaaaaatcg tatgaaaatt gttttttttt
      241 ctggattttt tgaagaaact tcacaaaaaa tttaggcggt aggcaggcag gtagacataa
      301 cttacgaaga agatggtacg ctcttggacg tatccctctg gcatggcgga cttgaagaag
      361 tcgtgacgct tcatgtggtc tgggtaacgg gagaagcatt ggactccgta gcagaaggtg
      421 gtgacgaggg ttggccatgg gactgggagc tttccggtgg tgcagatgaa cttgagggtg
      481 agctttccgt aggtggcgtc tccctctccc tctccggaga cggagaactt gtgtccgttg
      541 acgtctccgt cgagctcgac gaggattggg acgactccgg tgaagagctc ctctccctga
      601 aaatctattt gaaaaattta cgaggaaatt gttttttgta ctaaattttc aggaaaatct
      661 acaatttgag ccgaaaaggt gaaattttat ttaaaaactg caaaatttgg ggttaaaatc
      721 tggcaattct gaagaaaatt cgcttaaaaa cgcgatttca acgtagtttt tgattttttt
      781 tttttttgtt ttacagcgtg aaataatgaa tattcaacaa tttttggtga aaatcccgcg
      841 aaaaattcaa tttttcaagt aaaatctcgg ttttttcctt ctttcccata agaaaactta
      901 ccttggacac cttacgcttc ttctttggcg agcctgcttt tttgtacaaa cttgccatgg
      961 ctgctacgga gtgagcaaag tgtttcccaa ctgaaaaaa
//
