LOCUS       sequence-168068-         879 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses EQR-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pBK-EQR-BE3
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..769
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
       61 cactataggg agagccgcca ccatgagctc agagactggc ccagtggctg tggaccccac
      121 attgagacgg cggatcgagc cccatgagtt tgaggtattc ttcgatccga gagagctccg
      181 caaggagacc tgcctgcttt acgaaattaa ttgggggggc cggcactcca tttggcgaca
      241 tacatcacag aacactaaca agcacgtcga agtcaacttc atcgagaagt tcacgacaga
      301 aagatatttc tgtccgaaca caaggtgcag cattacctgg tttctcagct ggagcccatg
      361 cggcgaatgt agtagggcca tcactgaatt cctgtcaagg tatccccacg tcactctgtt
      421 tatttacatc gcaaggctgt accaccacgc tgacccccgc aatcgacaag gcctgcggga
      481 tttgatctct tcaggtgtga ctatccaaat tatgactgag caggagtcag gatactgctg
      541 gagaaacttt gtgaattata gcccgagtaa tgaagcccac tggcctaggt atccccatct
      601 gtgggtacga ctgtacgttc ttgaactgta ctgcatcata ctgggcctgc ctccttgtct
      661 caacattctg agaaggaagc agccacagct gacattcttt accatcgctc ttcagtcttg
      721 tcattaccag cgactgcccc cacacattct ctgggccacc gggttgaaaa gcggcagcga
      781 gactcccggg acctcagagt ccgccacacc cgaaagtgat aaaaagtatt ctattggttt
      841 agccatcggc actaattccg ttggatgggc tgtcataac
//
