LOCUS       sequence-168363-        3291 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing REG1B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_REG1B_p.K157T
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(691..790)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(807..826)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(808..826)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(831..847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(831..847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1278..2069
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2162..2750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2651..2670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2904..2921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3080..3107)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3199..3285)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggctcagacc aactcgttct tcatgctgat ctcctccctg atgttcctgt
      241 ctctgagcca aggccaggag tcccagacag agctgcctaa tccccgaatc agctgcccag
      301 aaggcaccaa tgcctatcgc tcctactgct actactttaa tgaagaccct gagacctggg
      361 ttgatgcaga tctctattgc cagaacatga attcaggcaa cctggtgtct gtgctcaccc
      421 aggcggaggg tgccttcgtg gcctcactga ttaaggagag tagcactgat gacagcaatg
      481 tctggattgg cctccatgac ccaaaaaaga accgccgctg gcactggagt agtgggtccc
      541 tggtctccta caagtcctgg gacactggat ccccgagcag tgctaatgct ggctactgtg
      601 caagcctgac ttcatgctca ggattcaaga aatggaagga tgaatcttgt gagaagacgt
      661 tctcctttgt ttgcaagttc aaaaactgat tgccaacttt cttgtacaaa gttgtcatta
      721 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      781 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
      841 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      901 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
      961 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     1021 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     1081 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1141 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1201 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1261 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1321 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1381 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     1441 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1501 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1561 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     1621 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     1681 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     1741 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     1801 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     1861 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     1921 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     1981 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     2041 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     2101 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2161 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2221 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2281 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2341 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2401 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2461 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2521 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2581 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2641 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2701 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2761 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2821 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2881 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2941 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3001 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3061 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3121 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3181 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3241 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t
//
