LOCUS       sequence-168627-        6000 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Donor vector containing PIK3CA, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CA_p.H1047Y
FEATURES             Location/Qualifiers
     source          1..6000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      26..614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     515..534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     768..785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(944..971)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1063..1149)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1198..1220
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1212..1229
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1213..1229
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1245..1344
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4555..4654)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4671..4690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4672..4690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4695..4711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4695..4711)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5142..5933
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
       61 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      901 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
      961 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     1021 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     1081 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     1141 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     1201 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat
     1261 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc
     1321 caactttgta caaaaaagtt ggcaccatgc ctccacgacc atcatcaggt gaactgtggg
     1381 gcatccactt gatgccccca agaatcctag tagaatgttt actaccaaat ggaatgatag
     1441 tgactttaga atgcctccgt gaggctacat taataaccat aaagcatgaa ctatttaaag
     1501 aagcaagaaa ataccccctc catcaacttc ttcaagatga atcttcttac attttcgtaa
     1561 gtgttactca agaagcagaa agggaagaat tttttgatga aacaagacga ctttgtgacc
     1621 ttcggctttt tcaacccttt ttaaaagtaa ttgaaccagt aggcaaccgt gaagaaaaga
     1681 tcctcaatcg agaaattggt tttgctatcg gcatgccagt gtgtgaattt gatatggtta
     1741 aagatccaga agtacaggac ttccgaagaa atattctgaa cgtttgtaaa gaagctgtgg
     1801 atcttaggga cctcaattca cctcatagta gagcaatgta tgtctatcct ccaaatgtag
     1861 aatcttcacc agaattgcca aagcacatat ataataaatt agataaaggg caaataatag
     1921 tggtgatctg ggtaatagtt tctccaaata atgacaagca gaagtatact ctgaaaatca
     1981 accatgactg tgtaccagaa caagtaattg ctgaagcaat caggaaaaaa actcgaagta
     2041 tgttgctatc ctctgaacaa ctaaaactct gtgttttaga atatcagggc aagtatattt
     2101 taaaagtgtg tggatgtgat gaatacttcc tagaaaaata tcctctgagt cagtataagt
     2161 atataagaag ctgtataatg cttgggagga tgcccaattt gatgttgatg gctaaagaaa
     2221 gcctttattc tcaactgcca atggactgtt ttacaatgcc atcttattcc agacgcattt
     2281 ccacagctac accatatatg aatggagaaa catctacaaa atccctttgg gttataaata
     2341 gtgcactcag aataaaaatt ctttgtgcaa cctacgtgaa tgtaaatatt cgagacattg
     2401 ataagatcta tgttcgaaca ggtatctacc atggaggaga acccttatgt gacaatgtga
     2461 acactcaaag agtaccttgt tccaatccca ggtggaatga atggctgaat tatgatatat
     2521 acattcctga tcttcctcgt gctgctcgac tttgcctttc catttgctct gttaaaggcc
     2581 gaaagggtgc taaagaggaa cactgtccat tggcatgggg aaatataaac ttgtttgatt
     2641 acacagacac tctagtatct ggaaaaatgg ctttgaatct ttggccagta cctcatggat
     2701 tagaagattt gctgaaccct attggtgtta ctggatcaaa tccaaataaa gaaactccat
     2761 gcttagagtt ggagtttgac tggttcagca gtgtggtaaa gttcccagat atgtcagtga
     2821 ttgaagagca tgccaattgg tctgtatccc gagaagcagg atttagctat tcccacgcag
     2881 gactgagtaa cagactagct agagacaatg aattaaggga aaatgacaaa gaacagctca
     2941 aagcaatttc tacacgagat cctctctctg aaatcactga gcaggagaaa gattttctat
     3001 ggagtcacag acactattgt gtaactatcc ccgaaattct acccaaattg cttctgtctg
     3061 ttaaatggaa ttctagagat gaagtagccc agatgtattg cttggtaaaa gattggcctc
     3121 caatcaaacc tgaacaggct atggaacttc tggactgtaa ttacccagat cctatggttc
     3181 gaggttttgc tgttcggtgc ttggaaaaat atttaacaga tgacaaactt tctcagtatt
     3241 taattcagct agtacaggtc ctaaaatatg aacaatattt ggataacttg cttgtgagat
     3301 ttttactgaa gaaagcattg actaatcaaa ggattgggca ctttttcttt tggcatttaa
     3361 aatctgagat gcacaataaa acagttagcc agaggtttgg cctgcttttg gagtcctatt
     3421 gtcgtgcatg tgggatgtat ttgaagcacc tgaataggca agtcgaggca atggaaaagc
     3481 tcattaactt aactgacatt ctcaaacagg agaagaagga tgaaacacaa aaggtacaga
     3541 tgaagttttt agttgagcaa atgaggcgac cagatttcat ggatgctcta cagggctttc
     3601 tgtctcctct aaaccctgct catcaactag gaaacctcag gcttgaagag tgtcgaatta
     3661 tgtcctctgc aaaaaggcca ctgtggttga attgggagaa cccagacatc atgtcagagt
     3721 tactgtttca gaacaatgag atcatcttta aaaatgggga tgatttacgg caagatatgc
     3781 taacacttca aattattcgt attatggaaa atatctggca aaatcaaggt cttgatcttc
     3841 gaatgttacc ttatggttgt ctgtcaatcg gtgactgtgt gggacttatt gaggtggtgc
     3901 gaaattctca cactattatg caaattcagt gcaaaggcgg cttgaaaggt gcactgcagt
     3961 tcaacagcca cacactacat cagtggctca aagacaagaa caaaggagaa atatatgatg
     4021 cagccattga cctgtttaca cgttcatgtg ctggatactg tgtagctacc ttcattttgg
     4081 gaattggaga tcgtcacaat agtaacatca tggtgaaaga cgatggacaa ctgtttcata
     4141 tagattttgg acactttttg gatcacaaga agaaaaaatt tggttataaa cgagaacgtg
     4201 tgccatttgt tttgacacag gatttcttaa tagtgattag taaaggagcc caagaatgca
     4261 caaagacaag agaatttgag aggtttcagg agatgtgtta caaggcttat ctagctattc
     4321 gacagcatgc caatctcttc ataaatcttt tctcaatgat gcttggctct ggaatgccag
     4381 aactacaatc ttttgatgac attgcataca ttcgaaagac cctagcctta gataaaactg
     4441 agcaagaggc tttggagtat ttcatgaaac aaatgaatga tgcatatcat ggtggctgga
     4501 caacaaaaat ggattggatc ttccacacaa ttaaacagca tgcattgaac tgattgccaa
     4561 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     4621 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     4681 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     4741 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     4801 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     4861 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     4921 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     4981 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     5041 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     5101 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     5161 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     5221 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     5281 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     5341 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     5401 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     5461 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     5521 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     5581 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     5641 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     5701 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     5761 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     5821 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     5881 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     5941 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
//
