LOCUS       sequence-168674-        4608 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Donor vector containing NFE2L2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NFE2L2_p.G31R
FEATURES             Location/Qualifiers
     source          1..4608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2487..2586)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2603..2622)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2604..2622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2627..2643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2627..2643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3074..3865
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3958..4546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4447..4466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa
      661 agttggcacc atgatggact tggagctgcc gccgccggga ctcccgtccc agcaggacat
      721 ggatttgatt gacatacttt ggaggcaaga tatagatctt agagtaagtc gagaagtatt
      781 tgacttcagt cagcgacgga aagagtatga gctggaaaaa cagaaaaaac ttgaaaagga
      841 aagacaagaa caactccaaa aggagcaaga gaaagccttt ttcgctcagt tacaactaga
      901 tgaagagaca ggtgaatttc tcccaattca gccagcccag cacatccagt cagaaaccag
      961 tggatctgcc aactactccc aggttgccca cattcccaaa tcagatgctt tgtactttga
     1021 tgactgcatg cagcttttgg cgcagacatt cccgtttgta gatgacaatg aggtttcttc
     1081 ggctacgttt cagtcacttg ttcctgatat tcccggtcac atcgagagcc cagtcttcat
     1141 tgctactaat caggctcagt cacctgaaac ttctgttgct caggtagccc ctgttgattt
     1201 agacggtatg caacaggaca ttgagcaagt ttgggaggag ctattatcca ttcctgagtt
     1261 acagtgtctt aatattgaaa atgacaagct ggttgagact accatggttc caagtccaga
     1321 agccaaactg acagaagttg acaattatca tttttactca tctataccct caatggaaaa
     1381 agaagtaggt aactgtagtc cacattttct taatgctttt gaggattcct tcagcagcat
     1441 cctctccaca gaagacccca accagttgac agtgaactca ttaaattcag atgccacagt
     1501 caacacagat tttggtgatg aattttattc tgctttcata gctgagccca gtatcagcaa
     1561 cagcatgccc tcacctgcta ctttaagcca ttcactctct gaacttctaa atgggcccat
     1621 tgatgtttct gatctatcac tttgcaaagc tttcaaccaa aaccaccctg aaagcacagc
     1681 agaattcaat gattctgact ccggcatttc actaaacaca agtcccagtg tggcatcacc
     1741 agaacactca gtggaatctt ccagctatgg agacacacta cttggcctca gtgattctga
     1801 agtggaagag ctagatagtg cccctggaag tgtcaaacag aatggtccta aaacaccagt
     1861 acattcttct ggggatatgg tacaaccctt gtcaccatct caggggcaga gcactcacgt
     1921 gcatgatgcc caatgtgaga acacaccaga gaaagaattg cctgtaagtc ctggtcatcg
     1981 gaaaacccca ttcacaaaag acaaacattc aagccgcttg gaggctcatc tcacaagaga
     2041 tgaacttagg gcaaaagctc tccatatccc attccctgta gaaaaaatca ttaacctccc
     2101 tgttgttgac ttcaacgaaa tgatgtccaa agagcagttc aatgaagctc aacttgcatt
     2161 aattcgggat atacgtagga ggggtaagaa taaagtggct gctcagaatt gcagaaaaag
     2221 aaaactggaa aatatagtag aactagagca agatttagat catttgaaag atgaaaaaga
     2281 aaaattgctc aaagaaaaag gagaaaatga caaaagcctt cacctactga aaaaacaact
     2341 cagcacctta tatctcgaag ttttcagcat gctacgtgat gaagatggaa aaccttattc
     2401 tcctagtgaa tactccctgc agcaaacaag agatggcaat gttttccttg ttcccaaaag
     2461 taagaagcca gatgttaaga aaaactaccc aactttcttg tacaaagttg gcattataag
     2521 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2581 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2641 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2701 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     2761 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     2821 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     2881 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2941 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     3001 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     3061 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     3121 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     3181 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     3241 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     3301 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     3361 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     3421 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     3481 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     3541 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     3601 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     3661 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3721 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     3781 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     3841 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     3901 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4081 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
