LOCUS       sequence-169004-        4122 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Donor vector containing EDNRB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_EDNRB_WT
FEATURES             Location/Qualifiers
     source          1..4122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1522..1621)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1638..1657)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1639..1657)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1662..1678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1662..1678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2109..2900
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2993..3581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3482..3501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3735..3752
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3911..3938)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4030..4116)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcagccg cctccaagtc tgtgcggacg cgccctggtt gcgctggttc
      241 ttgcctgcgg cctgtcgcgg atctggggag aggagagagg cttcccgcct gacagggcca
      301 ctccgctttt gcaaaccgca gagataatga cgccacccac taagacctta tggcccaagg
      361 gttccaacgc cagtctggcg cggtcgttgg cacctgcgga ggtgcctaaa ggagacagga
      421 cggcaggatc tccgccacgc accatctccc ctcccccgtg ccaaggaccc atcgagatca
      481 aggagacttt caaatacatc aacacggttg tgtcctgcct tgtgttcgtg ctggggatca
      541 tcgggaactc cacacttctg agaattatct acaagaacaa gtgcatgcga aacggtccca
      601 atatcttgat cgccagcttg gctctgggag acctgctgca catcgtcatt gacatcccta
      661 tcaatgtcta caagctgctg gcagaggact ggccatttgg agctgagatg tgtaagctgg
      721 tgcctttcat acagaaagcc tccgtgggaa tcactgtgct gagtctatgt gctctgagta
      781 ttgacagata tcgagctgtt gcttcttgga gtagaattaa aggaattggg gttccaaaat
      841 ggacagcagt agaaattgtt ttgatttggg tggtctctgt ggttctggct gtccctgaag
      901 ccataggttt tgatataatt acgatggact acaaaggaag ttatctgcga atctgcttgc
      961 ttcatcccgt tcagaagaca gctttcatgc agttttacaa gacagcaaaa gattggtggc
     1021 tgttcagttt ctatttctgc ttgccattgg ccatcactgc atttttttat acactaatga
     1081 cctgtgaaat gttgagaaag aaaagtggca tgcagattgc tttaaatgat cacctaaagc
     1141 agagacggga agtggccaaa accgtctttt gcctggtcct tgtctttgcc ctctgctggc
     1201 ttccccttca cctcagcagg attctgaagc tcactcttta taatcagaat gatcccaata
     1261 gatgtgaact tttgagcttt ctgttggtat tggactatat tggtatcaac atggcttcac
     1321 tgaattcctg cattaaccca attgctctgt atttggtgag caaaagattc aaaaactgct
     1381 ttaagtcatg cttatgctgc tggtgccagt catttgaaga aaaacagtcc ttggaggaaa
     1441 agcagtcgtg cttaaagttc aaagctaatg atcacggata tgacaacttc cgttccagta
     1501 ataaatacag ctcatcttga ttgccaactt tcttgtacaa agttggcatt ataagaaagc
     1561 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1621 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     1681 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1741 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     1801 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     1861 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     1921 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1981 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2041 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2101 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2161 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2221 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     2281 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2341 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2401 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2461 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2521 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2581 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2641 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     2701 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     2761 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     2821 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     2881 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     2941 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3001 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3061 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3121 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3361 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3601 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3661 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3721 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3781 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3841 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     3901 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     3961 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     4021 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     4081 ggcccagtct tccgactgag cctttcgttt tatttgatgc ct
//
