LOCUS       sequence-169157-        4977 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Donor vector containing YTHDC1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_YTHDC1_WT
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2856..2955)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2972..2991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2973..2991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2996..3012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2996..3012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3443..4234
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4327..4915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4816..4835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcggctg acagtcggga ggagaaagat ggagaactta atgttctgga
      721 tgatatttta actgaagtac cagaacaaga tgatgaactg tataatccag agagtgaaca
      781 agataaaaat gagaaaaagg gatcaaaaag aaaaagtgat cgaatggaat ctactgatac
      841 caaacgacaa aagccttctg tccattcaag acaactggtt tctaagccac tgagctcatc
      901 tgttagcaat aacaaaagaa tagttagtac aaaaggaaag tcagccacag agtataaaaa
      961 tgaggaatat caaagatctg aaagaaacaa gcgtctagat gctgatcgga aaattcgtct
     1021 atcaagtagt gcctccagag aaccttataa gaatcaacct gaaaaaacct gtgtccggaa
     1081 aagggatcct gaaaggaggg ccaaatctcc tacgccagat ggttctgaga gaattgggct
     1141 tgaagtggat agacgtgcaa gcagatccag ccagtcttct aaggaagaag tgaactctga
     1201 agaatatggc tctgaccatg agactggcag cagtggttct tctgatgagc aagggaacaa
     1261 cactgagaat gaggaggaag gagtggaaga agatgtggag gaagatgaag aagtagaaga
     1321 agatgcagaa gaagatgaag aggtggatga agatggagag gaggaggagg aagaggagga
     1381 ggaggaagag gaggaggagg aggaggaaga agaagaatat gaacaggatg agagagacca
     1441 gaaagaggag ggaaatgatt atgacactcg aagtgaggcc agtgactctg gttctgaatc
     1501 tgtttccttc acagatgggt ctgtcagatc tggttcaggc acagatggat cagatgagaa
     1561 aaagaaggaa aggaagagag ctagaggcat atctccaatt gtttttgata gaagtggaag
     1621 ctctgcatca gagtcatatg caggttcaga aaagaagcat gagaaattat catcttccgt
     1681 tcgtgctgtc cgaaaagatc aaaccagtaa actcaaatat gtgcttcaag atgcaagatt
     1741 tttcctcata aagagtaaca accatgagaa tgtgtctctt gccaaagcga agggtgtatg
     1801 gtccacgctc cctgtaaatg agaagaaatt aaatcttgca tttagatctg caaggagtgt
     1861 tatcttaata ttttctgtca gagagagtgg aaaatttcaa gggtttgcaa gactttcttc
     1921 agaatcacat cacggaggat ctcctataca ctgggtgctt ccagcaggaa tgagtgctaa
     1981 aatgctggga ggtgtcttta aaattgactg gatttgcagg cgtgaattac ccttcactaa
     2041 gtcggctcat ctcaccaatc cttggaatga acataaacca gtaaagatcg gacgtgatgg
     2101 acaggaaatt gaacttgaat gtggaaccca gctttgtctt ctgtttcccc ccgatgaaag
     2161 tattgacttg tatcaggtca ttcataaaat gcgtcacaag agaagaatgc attctcagcc
     2221 ccgatcacga ggacgtccat cccgtcgaga accagtccgg gatgtgggaa ggcgtcgacc
     2281 agaagattat gatattcata acagcagaaa gaaaccaagg attgactatc cccctgagtt
     2341 tcaccagaga ccagggtatt taaaggatcc acgataccag gaagtggaca gacgattttc
     2401 aggagttcgc cgagatgtgt ttttaaatgg gtcctacaat gattatgtga gggaatttca
     2461 taacatggga ccaccaccac cttggcaagg aatgccccct tacccaggaa tggaacaacc
     2521 tccacaccat ccttactatc agcaccatgc tccacctcct caagctcatc ccccttactc
     2581 aggacatcat ccagtaccac atgaagcaag atacagagat aaacgagtac atgattatga
     2641 tatgagggtg gatgatttcc ttcgtcgcac acaagctgtt gtcagtggcc ggagaagtag
     2701 accccgtgaa agagaccggg aacgagagcg agaccgccct agagataaca gacgagacag
     2761 agagcgagat agaggacgtg atagagaaag agaaagagag cgattatgtg atcgagacag
     2821 agaccgaggg gagagaggtc gatatagaag atgattgcca actttcttgt acaaagttgg
     2881 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2941 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     3001 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3061 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     3121 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     3181 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     3241 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3301 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     3361 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     3421 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     3481 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     3541 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3601 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     3661 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     3721 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     3781 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     3841 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     3901 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     3961 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     4021 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     4081 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     4141 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     4201 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     4261 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4321 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4381 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4441 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4501 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4561 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4621 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4681 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4741 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4801 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4861 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4921 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
