LOCUS       sequence-169165-       14834 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14834)
  AUTHORS   Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP
  TITLE     An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis 
            thaliana.
  JOURNAL   Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. 
            eCollection 2017.
  PUBMED    28174584
REFERENCE   2  (bases 1 to 14834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-01-24"; volume: "8:39. doi"; pages: " 
            10.3389/fpls.2017.00039. eCollection 2017"
COMMENT     Sequence Label: pUB-Cas9-@GL1
FEATURES             Location/Qualifiers
     source          1..14834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    40..214
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    292..316
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             741..1535
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1622..2210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2111..2130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2364..2381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2396..2536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2462..2481)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2622..2644
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2880..3074
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3140..4207)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(4641..5270)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6570..6594
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6782..6804
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6796..6813
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6797..6813
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6846..6855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6850..6859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_RNA        7356..7431
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8235..8259
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8280..8300
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10017..12401
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    complement(12406..12430)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(12759..12775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12759..12775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12772..12794)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12783..12799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12807..12813,12814..12831,12832..12837))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12852..12873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13064..13741
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             13809..14834
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
ORIGIN
        1 agtagatgcc gaccggatct gtcgatcgac aagctcgagt ttctccataa taatgtgtga
       61 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
      121 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
      181 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
      241 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
      301 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
      361 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
      421 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
      481 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
      541 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
      601 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
      661 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
      721 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
      781 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
      841 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
      901 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
      961 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     1021 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     1081 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     1141 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
     1201 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
     1261 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
     1321 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
     1381 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
     1441 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     1501 taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc
     1561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1741 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2401 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     2461 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     2521 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2581 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2641 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     2701 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     2761 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     2821 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     2881 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     2941 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     3001 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     3061 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     3121 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     3181 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     3241 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     3301 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     3361 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     3421 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     3481 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     3541 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     3601 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     3661 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     3721 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     3781 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     3841 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     3901 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     3961 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     4021 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     4081 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     4141 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     4201 tgcgaccgtc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     4261 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     4321 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     4381 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     4441 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     4501 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     4561 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     4621 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     4681 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     4741 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     4801 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     4861 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     4921 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     4981 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     5041 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     5101 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     5161 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     5221 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     5281 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     5341 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     5401 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     5461 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     5521 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     5581 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     5641 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     5701 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     5761 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     5821 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     5881 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     5941 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     6001 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     6061 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     6121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     6181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     6241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     6301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     6361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     6421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     6481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     6541 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
     6601 gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat
     6661 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6721 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6781 tcccagtcac gacgttgtaa aacgacggcc agtgccagaa ttgggcccga cgtcgcatgc
     6841 tcccggccgc catggcggcc gcgggaattc gattcacgaa gcttcgttga acaacggaaa
     6901 ctcgacttgc cttccgcaca atacatcatt tcttcttagc tttttttctt cttcttcgtt
     6961 catacagttt ttttttgttt atcagcttac attttcttga accgtagctt tcgttttctt
     7021 ctttttaact ttccattcgg agtttttgta tcttgtttca tagtttgtcc caggattaga
     7081 atgattaggc atcgaacctt caagaatttg attgaataaa acatcttcat tcttaagata
     7141 tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg cccatttata
     7201 tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca atcttcaaaa
     7261 gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta
     7321 gtgattgtcc cttcgggaaa agttgtagac tgagagtttt agagctagaa atagcaagtt
     7381 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg caaatttttt
     7441 taaactagtg gtgatcttgc tgaaaaactc gagccatccg gaagatcttg aattcgcggc
     7501 cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttggtaccc
     7561 gacgagtcag taataaacgg cgtcaaagtg gttgcagccg gcacacacga gtcgtgttta
     7621 tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata aggcaattag ccaaaaacaa
     7681 ctttgcgtgt aaacaacgct caatacacgt gtcattttat tattagctat tgcttcaccg
     7741 ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt cttcttcttc tataaaacaa
     7801 tacccaaaga gctcttcttc ttcacaattc agatttcaat ttctcaaaat cttaaaaact
     7861 ttctctcaat tctctctacc gtgatcaagg taaatttctg tgttccttat tctctcaaaa
     7921 tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt tagattctgt
     7981 taatcttaga tcgaagacga ttttctgggt ttgatcgtta gatatcatct taattctcga
     8041 ttagggtttc atagatatca tccgatttgt tcaaataatt tgagttttgt cgaataatta
     8101 ctcttcgatt tgtgatttct atctagatct ggtgttagtt tctagtttgt gcgatcgaat
     8161 ttgtagatta atctgagttt ttctgattaa cagctcgagt gcgggccccc cctcgaggcg
     8221 cgccaagcta tcaaacaagt ttgtacaaaa aagcaggcta ccatatggcc agcagccccc
     8281 ccaagaagaa gcgcaaggtc gacaagaagt actccatcgg cctggacatc ggcacgaaca
     8341 gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc gtccaagaag ttcaaggtgc
     8401 tgggcaacac cgaccgccac agcatcaaga agaacctgat cggcgccctg ctgttcgact
     8461 cgggcgagac cgccgaggcg acgcgcctga agcgcaccgc gcgccgccgc tacacgcgcc
     8521 gcaagaaccg catctgctac ctgcaggaga tcttcagcaa cgagatggcc aaggtggacg
     8581 actcgttctt ccaccgcctg gaggagtcct tcctggtgga ggaggacaag aagcacgagc
     8641 gccaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag tacccgacga
     8701 tctaccacct gcgcaagaag ctggtggaca gcaccgacaa ggcggacctg cgcctgatct
     8761 acctggccct ggcgcacatg atcaagttcc gcggccactt cctgatcgag ggcgacctga
     8821 accccgacaa ctcggacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     8881 tgttcgagga gaacccgatc aacgcctcgg gcgtggacgc caaggcgatc ctgtccgcgc
     8941 gcctgtccaa gagccgccgc ctggagaacc tgatcgccca gctgcccggc gagaagaaga
     9001 acggcctgtt cggcaacctg atcgcgctga gcctgggcct gacgccgaac ttcaagtcga
     9061 acttcgacct ggccgaggac gcgaagctgc agctgagcaa ggacacctac gacgacgacc
     9121 tggacaacct gctggcccag atcggcgacc agtacgcgga cctgttcctg gccgcgaaga
     9181 acctgtcgga cgccatcctg ctgtccgaca tcctgcgcgt gaacaccaag atcacgaagg
     9241 cccccctgtc ggcgtccatg atcaagcgct acgacgagca ccaccaggac ctgaccctgc
     9301 tgaaggcgct ggtgcgccag cagctgccgg agaagtacaa ggagatcttc ttcgaccagt
     9361 ccaagaacgg ctacgccggc tacatcgacg gcggcgccag ccaggaggag ttctacaagt
     9421 tcatcaagcc catcctggag aagatggacg gcacggagga gctgctggtg aagctgaacc
     9481 gcgaggacct gctgcgcaag cagcgcacct tcgacaacgg cagcatcccc caccagatcc
     9541 acctgggcga gctgcacgcc atcctgcgcc gccaggagga cttctacccg ttcctgaagg
     9601 acaaccgcga gaagatcgag aagatcctga cgttccgcat cccctactac gtgggcccgc
     9661 tggctcgcgg caactcccgc ttcgcgtgga tgacccgcaa gagcgaggag accatcacgc
     9721 cctggaactt cgaggaggtg gtggacaagg gcgccagcgc gcagtcgttc atcgagcgca
     9781 tgaccaactt cgacaagaac ctgcccaacg agaaggtgct gccgaagcac tccctgctgt
     9841 acgagtactt caccgtgtac aacgagctga cgaaggtgaa gtacgtgacc gagggcatgc
     9901 gcaagcccgc cttcctgagc ggcgagcaga agaaggcgat cgtggacctg ctgttcaaga
     9961 ccaaccgcaa ggtgacggtg aagcagctga aggaggacta cttcaagaag atcgagtgct
    10021 tcgacagcgt ggagatctcg ggcgtggagg accgcttcaa cgccagcctg ggcacctacc
    10081 acgacctgct gaagatcatc aaggacaagg acttcctgga caacgaggag aacgaggaca
    10141 tcctggagga catcgtgctg accctgacgc tgttcgagga ccgcgagatg atcgaggagc
    10201 gcctgaagac gtacgcccac ctgttcgacg acaaggtgat gaagcagctg aagcgccgcc
    10261 gctacaccgg ctggggccgc ctgagccgca agctgatcaa cggcatccgc gacaagcagt
    10321 cgggcaagac catcctggac ttcctgaagt ccgacggctt cgcgaaccgc aacttcatgc
    10381 agctgatcca cgacgactcg ctgaccttca aggaggacat ccagaaggcc caggtgtcgg
    10441 gccagggcga ctccctgcac gagcacatcg ccaacctggc gggctccccc gcgatcaaga
    10501 agggcatcct gcagaccgtg aaggtggtgg acgagctggt gaaggtgatg ggccgccaca
    10561 agccggagaa catcgtgatc gagatggccc gcgagaacca gaccacgcag aagggccaga
    10621 agaacagccg cgagcgcatg aagcgcatcg aggagggcat caaggagctg ggctcgcaga
    10681 tcctgaagga gcaccccgtg gagaacaccc agctgcagaa cgagaagctg tacctgtact
    10741 acctgcagaa cggccgcgac atgtacgtgg accaggagct ggacatcaac cgcctgtccg
    10801 actacgacgt ggaccacatc gtgccccaga gcttcctgaa ggacgactcg atcgacaaca
    10861 aggtgctgac ccgcagcgac aagaaccgcg gcaagagcga caacgtgccg tcggaggagg
    10921 tggtgaagaa gatgaagaac tactggcgcc agctgctgaa cgccaagctg atcacgcagc
    10981 gcaagttcga caacctgacc aaggccgagc gcggcggcct gtcggagctg gacaaggcgg
    11041 gcttcatcaa gcgccagctg gtggagaccc gccagatcac gaagcacgtg gcgcagatcc
    11101 tggactcccg catgaacacg aagtacgacg agaacgacaa gctgatccgc gaggtgaagg
    11161 tgatcaccct gaagtccaag ctggtcagcg acttccgcaa ggacttccag ttctacaagg
    11221 tgcgcgagat caacaactac caccacgccc acgacgcgta cctgaacgcc gtggtgggca
    11281 ccgcgctgat caagaagtac cccaagctgg agagcgagtt cgtgtacggc gactacaagg
    11341 tgtacgacgt gcgcaagatg atcgccaagt cggagcagga gatcggcaag gccaccgcga
    11401 agtacttctt ctactccaac atcatgaact tcttcaagac cgagatcacg ctggccaacg
    11461 gcgagatccg caagcgcccc ctgatcgaga ccaacggcga gacgggcgag atcgtgtggg
    11521 acaagggccg cgacttcgcg accgtgcgca aggtgctgag catgccccag gtgaacatcg
    11581 tgaagaagac cgaggtgcag acgggcggct tctccaagga gagcatcctg ccgaagcgca
    11641 actcggacaa gctgatcgcc cgcaagaagg actgggaccc caagaagtac ggcggcttcg
    11701 actccccgac cgtggcctac agcgtgctgg tggtggcgaa ggtggagaag ggcaagtcca
    11761 agaagctgaa gagcgtgaag gagctgctgg gcatcaccat catggagcgc agctcgttcg
    11821 agaagaaccc catcgacttc ctggaggcga agggctacaa ggaggtgaag aaggacctga
    11881 tcatcaagct gccgaagtac tcgctgttcg agctggagaa cggccgcaag cgcatgctgg
    11941 cctccgcggg cgagctgcag aagggcaacg agctggccct gcccagcaag tacgtgaact
    12001 tcctgtacct ggcgtcccac tacgagaagc tgaagggctc gccggaggac aacgagcaga
    12061 agcagctgtt cgtggagcag cacaagcact acctggacga gatcatcgag cagatctcgg
    12121 agttctccaa gcgcgtgatc ctggccgacg cgaacctgga caaggtgctg agcgcctaca
    12181 acaagcaccg cgacaagccc atccgcgagc aggcggagaa catcatccac ctgttcaccc
    12241 tgacgaacct gggcgccccg gccgcgttca agtacttcga caccacgatc gaccgcaagc
    12301 gctacaccag cacgaaggag gtgctggacg cgaccctgat ccaccagagc atcaccggcc
    12361 tgtacgagac gcgcatcgac ctgtcgcagc tgggcggcga ctaataccca gctttcttgt
    12421 acaaagtggt tcgataattc cttaattaac tagttctaga gcggccgccc accgcggtgg
    12481 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
    12541 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
    12601 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
    12661 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
    12721 cgcgcggtgt catctatgtt actgaattcg taatcatggt catagctgtt tcctgtgtga
    12781 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    12841 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    12901 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    12961 ggtttgcgta ttggctagag cagcttgcca acatggtgga gcacgacact ctcgtctact
    13021 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
    13081 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
    13141 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
    13201 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
    13261 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgataaca
    13321 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
    13381 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
    13441 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
    13501 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
    13561 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
    13621 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
    13681 atccttcgca agaccttcct ctatataagg aagttcattt catttggaga ggacacgctg
    13741 aaatcaccag tctctctcta caaatctatc tctctcgagc tttcgcagat cccggggggc
    13801 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
    13861 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
    13921 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
    13981 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
    14041 ttgacattgg ggagtttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
    14101 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg
    14161 ctatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
    14221 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    14281 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    14341 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    14401 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    14461 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    14521 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    14581 gatcgccacg actccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    14641 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    14701 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    14761 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
    14821 gggcaaagaa atag
//
