LOCUS       sequence-169547-        4303 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Secreted Luciferase reporter for PPARgamma activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Degrelle SA, Shoaito H, Fournier T
  TITLE     New Transcriptional Reporters to Quantify and Monitor PPARgamma 
            Activity.
  JOURNAL   PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 
            Nov 1.
  PUBMED    29225614
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PPAR Res.";
            date: "2017"; volume: "2017:6139107. doi"; pages: " 
            10.1155/2017/6139107. Epub 2017 Nov 1"
COMMENT     Sequence Label: PPRE-pNL1.3
FEATURES             Location/Qualifiers
     source          1..4303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1253..1765
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     polyA_signal    1814..1935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1825..1844)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1879..1898
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2183..2200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2354..2942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2434..2453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3142..3933,3934..4002))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4107..4155
                     /note="synthetic polyadenylation signal"
     misc_feature    4169..4260
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4209..4228
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 cagagccggg ccactttctc aggcctctga tttcatactg tggtgttagt tacttctgag
       61 aggacagctt gctgccagag ctctattttt tatgttagag gctccttctg cctgcagact
      121 ctgctgtctg ggaagggcac agcgttagga gggagaggga ggtgtgagtc cctccgtgga
      181 cccgctgctt tgtacttctc tatctcattt ccttttcagc accactctgg gaaatcagta
      241 ttccagcccc attttatcct cagaaaattg aggctctgag atgttatctc tgtgacctgg
      301 gtcctattac gtgccaaagg catcatttaa gcctaagatg tcctggctcc aaggtgtcag
      361 catctggaag acaggcgccc tcatcctgcc atccctgctg cggcttcact gtgggcccag
      421 gggacatctc agccccgaga agggtcagcg gcccctcctg gaccaccgac tccccgcaga
      481 actcctctgt gccctctcct caccagacct tgttcctccc agttgctccc acagccaggg
      541 ggcagtgagg gctgctcttc ccccagcccc actgaggaac ccaggaaggt gaacgagaga
      601 atcagtcctg gtgggggctg gggagggccc cagacatgag accagctcct cccccagggg
      661 atgttatcag tgggtccaga gggcaaaata gggagcctgg tggagggagg ggcctgaaat
      721 tccagtgtga aaggctgaga tgggcccgag gcccctggcc tatgtccaag ccatttcccc
      781 tctcaccagc ctctccctgg ggagccagtc agctaggaag gaatgagggc tccccaggcc
      841 caccccagtt cctgagctca tctgggctgc agggctggcg ggacagcagc gtggactcag
      901 tctcctaggg atttcccaac tctcccgccc gcttgctgca tctggacacc ctgcctcagg
      961 ccctcatctc cactggtcag caggtgacct ttgcccagcg ccctgggtcc tcagtgcctg
     1021 ctgccctgga gatgatataa aacaggtcag aaccctcctg cctgtctgct cagttcatcc
     1081 ctagaggcag gactctagag gatctgcgat cagatctggc ctcggcggcc aagcttggca
     1141 atccggtact gttggtaaag ccaccatgaa ctccttctcc acaagcgcct tcggtccagt
     1201 tgccttctcc ctgggcctgc tcctggtgtt gcctgctgcc ttccctgccc cagtcttcac
     1261 actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct
     1321 tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca
     1381 aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta
     1441 tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc
     1501 tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt
     1561 tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg
     1621 caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct
     1681 gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg
     1741 gctgtgcgaa cgcattctgg cgtaaggccg cgactctaga gtcggggcgg ccggccgctt
     1801 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     1861 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     1921 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     1981 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     2041 ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     2101 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     2161 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2761 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca
     3121 aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat
     3181 ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg
     3241 tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc
     3301 ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc
     3361 tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc
     3421 gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc
     3481 gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc
     3541 acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa
     3601 gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat
     3661 gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta
     3721 gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca
     3781 tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag
     3841 gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc
     3901 agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc
     3961 aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata
     4021 ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa
     4081 ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg
     4141 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     4201 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     4261 ggcctaactg gccggtacca tgcctgcagg tcaattctga ggg
//
