LOCUS       sequence-169578-        8807 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encrypted AND gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8807)
  TITLE     Tim Lu lab plasmids
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOP437
FEATURES             Location/Qualifiers
     source          1..8807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(519..1469)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1517..1739
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2243..2337)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2367..3158,3159..3227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2990..3009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3228..3332)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    3436..3469
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    3619..3668
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     terminator      complement(3682..3768)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3774..4652)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        complement(join(4822..4847,4848..4853,4854..4870,4871..4876,
                     4877..4895))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    4852..4870
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4877..4895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4958..4974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4988..5007
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     CDS             complement(5185..5214)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    5416..5432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5435..5492
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             5619..6701
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5638..5657)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      6707..6793
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        join(6807..6825,6826..6831,6832..6848,6849..6854,
                     6855..6880)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    6807..6825
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6832..6850
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      6993..7087
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(7094..7717)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     7625..7644
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_RNA        7881..7930
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(8014..8042)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8637..8657
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      complement(8707..8801)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt tatcagaatc
       61 gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag aattgccatg
      121 attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt gattcgataa
      181 gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca agttgtctca
      241 ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt ctattaggtg
      301 ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct ttgaatgcac
      361 caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata
      421 tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg tttgttagtc
      481 ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc
      541 agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga
      601 tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt
      661 gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtagga atctgatgta
      721 atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg
      781 agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc
      841 ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa
      901 acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta
      961 aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt
     1021 aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga
     1081 ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact
     1141 aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct
     1201 ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct
     1261 aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg
     1321 aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca
     1381 cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca
     1441 tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca
     1501 ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct ttgagttgtg
     1561 ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct
     1621 gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat
     1681 agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca
     1741 ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta
     1801 caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg
     1861 ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat
     1921 tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct
     1981 tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc
     2041 agggcgtttt atggtgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt
     2101 cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac
     2161 tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtccc
     2221 tagtatcgat actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     2281 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     2341 gcgagctcgt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2401 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2461 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2521 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2581 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2641 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2701 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2761 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2821 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2881 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2941 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3001 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3061 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3121 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3181 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3241 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3301 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3361 gtccttacgt gccgatcaac gtctcatttt cgccagatat cgacgtcaaa gattctagga
     3421 tctaaccgta tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actcccacag
     3481 ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct ggataacttt
     3541 acgggcatgc ataaggctcg tataatatat tcagggagac cacaacggtt tccctctaca
     3601 aataattttg tttaactttc ggccggcttg tcgacgacgg cggtctccgt cgtcaggatc
     3661 atccgggctc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     3721 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gccttatgac
     3781 aacttgacgg ctacatcatt cactttttct tcacaaccgg cacgaaactc gctcgggctg
     3841 gccccggtgc attttttaaa tactcgcgag aaatagagtt gatcgtcaaa accaacattg
     3901 cgaccgacgg tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctggctgata
     3961 cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac
     4021 agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct
     4081 gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggcggatgg
     4141 agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc
     4201 agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg
     4261 ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc aaatattgac
     4321 ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac
     4381 cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa
     4441 atatcacccg gtcggcagac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga
     4501 atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga
     4561 taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc
     4621 ggcagcaggg gatcattttg cgcttcagcc atgctagctc tcgtccctag cttcgccgaa
     4681 ggcgttaaac aaaattattt gtagaggggg tttcgtcatc actgactcat cagagttgaa
     4741 agcacatcaa ctgacccctg tttgtaaagg agactgataa tggcatgtac aactatactc
     4801 gtcggtaaaa aggcatctta tggtcagtgc gtcctgctga tgtgctcagt atctctatca
     4861 ctgataggga tgtcaatctc tatcactgat agggaatgcc aacacaatta acatctcaat
     4921 caaggtaaat gctttttgct ttttttgctg tgtgaaattg ttatccgctc acaattgaat
     4981 ctatcataat tgtgagcgct cacaattgta aagcttagga tccgctaatc ttatggataa
     5041 aaatgctatg ctcgagtcga cagttcatag gtgattgctc aggacatttc tgttagaagg
     5101 aatcgttttc cttacttttc cttacgcaca agagttccgt agctgttcaa gtttgtgttt
     5161 caactgttct cgtcgtttcc gcaacaagtc ctcttcagaa atgagctttt gctcctctgc
     5221 ttggacggac aggatgtatg ctgtggcttt tttaaggata actaccttgg gggccttttc
     5281 attgttttcc aactccggga tctggtcacg cagggcaaaa aagctccgtt ttagctcgtt
     5341 cctcctctgg cgctccaaga cgttgtgtgt tcgcctcttg acattctcct cggtgtccga
     5401 gggccctgtg tgaaattgtt atccgctcac aattgtcgtg gtttgtctgg tcaaccaccg
     5461 cggtctcagt ggtgtacggt acaaaccccg acagtagtca ccggctgtgc ttgccggtct
     5521 gatgagcctg tgaaggcgaa actacctcta caaataattt tgtttaagca tgccagttgt
     5581 agatcagcat agcatagaca accaaggagg tagtgcacat gaaaccagta acgctgtacg
     5641 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     5701 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     5761 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     5821 cctcttctct ggctctgcac gctccgtctc agatcgttgc tgctatcaaa tctcgtgctg
     5881 accagctggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     5941 aagcggcggt tcacaatctt ctcgcgcagc gcgtcagtgg gctgatcatt aactatccgc
     6001 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     6061 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     6121 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     6181 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     6241 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     6301 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     6361 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggaca
     6421 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     6481 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     6541 ctcagggcca ggcggtgaag ggcaatcaac tgttgcccgt ctcactggtg aaaagaaaaa
     6601 ccaccctggc tcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6661 aactggcacg acaggtttcc cgactggaaa gcgggcagta aggcatcaaa taaaacgaaa
     6721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     6781 gagtaggaca aatccgccgc cctagatccc tatcagtgat agagattgac atccctatca
     6841 gtgatagaga tactgagcac atcagcagga cgcactgacc gtgccccaac tggggtaacc
     6901 tttgagttct ctcagttggg ggaagcatta tttaggcact acaactagta tagttgtaca
     6961 tgaaaaacgc tgtatttttt tatccatgct tggactcctg ttgatagatc cagtaatgac
     7021 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     7081 tgagaatcca agctcaagac ccactttcac atttaagttg tttttctaat ccgcagatga
     7141 tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag
     7201 cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga
     7261 cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg
     7321 catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga
     7381 gagtttcata ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat
     7441 gacttagtaa agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt
     7501 ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt
     7561 cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct
     7621 tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg
     7681 cgctgttaat cactttactt ttatctaaac gagacatcgg cgcctcccta tcagttgaac
     7741 gatagttaat actttaaaca aaattatttg tagaggcggt ttcgacatca ctgtctcatc
     7801 agaacgcaaa gcacatgcgt tgaccgctaa aggagttttt tagttacctt aattgaaata
     7861 aacgaaataa aaactcgcag ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag
     7921 gacgaaacag cctctacaaa taattttgtt taaataggtc ccaatataag gagagggtgt
     7981 acaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     8041 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     8101 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     8161 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     8221 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     8281 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     8341 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     8401 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     8461 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     8521 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     8581 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     8641 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgatct ctacaaataa
     8701 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     8761 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcg
//
