LOCUS       sequence-169611-       13324 bp    DNA     circular SYN 14-APR-2025
DEFINITION  FLS2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13324)
  AUTHORS   Robatzek S, Chinchilla D, Boller T
  TITLE     Ligand-induced endocytosis of the pattern recognition receptor FLS2 
            in Arabidopsis.
  JOURNAL   Genes Dev. 2006 Mar 1;20(5):537-42.
  PUBMED    16510871
REFERENCE   2  (bases 1 to 13324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2006-03-1"; volume: "20(5)"; pages: "537-42"
COMMENT     Sequence Label: pCAMBIA-FLS2prom::FLS2-3xmyc
FEATURES             Location/Qualifiers
     source          1..13324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3613..3642
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3649..3678
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3685..3714
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3959..3976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3959..3975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3968..3990)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4178..4202
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5503..6132
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6561..7634
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7700..7894
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8130..8152)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8238..8378
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8293..8312
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8393..8410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8564..9152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8644..8663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9239..10033)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10458..10482
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10560..10734
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10791..11588)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10902..10921)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11512..11531
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(11657..12002)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(11694..11714)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    12193..12214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12229..12234,12235..12252,12253..12259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12267..12283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12272..12294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12291..12307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12291..12307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaagttac tctcaaagac ctttttgata ttaactctca ccttcttctt ctttggcatt
       61 gcactagcga aacagagctt tgaaccagag atcgaagctt tgaaatcctt caagaatggt
      121 atttccaacg accctttagg agtattatca gattggacca tcatcggttc gttacgacac
      181 tgtaattgga ccggaatcac ctgcgatagt accggacatg tagtctcggt ttccttgctg
      241 gagaagcaac ttgaaggtgt cctgtctcca gccatagcga atctcaccta tctccaggtt
      301 cttgatctca cttcaaatag ttttaccggc aaaataccgg ctgaaatagg aaagttaacc
      361 gagcttaacc agcttattct gtacctaaac tatttctctg gttcgattcc ttctggaatc
      421 tgggagctta agaatatttt ctatcttgat cttagaaata atttgttgtc cggtgatgtt
      481 cctgaggaaa tctgcaaaac cagttctttg gtattgattg ggtttgatta caacaactta
      541 accgggaaaa taccagaatg cttaggagat ttggttcatc tccaaatgtt tgtagcagct
      601 ggtaaccatt taactggttc gattccggta tcaattggta ctctggctaa tttaacggat
      661 ttagacctga gtggtaacca gttaaccgga aaaataccga gagattttgg aaatctcttg
      721 aacttacagt ctctcgtttt aactgaaaac ttgttggaag gagatatacc agctgagatc
      781 ggaaactgct cgagcttggt ccaacttgag ctttacgata accagttaac cgggaaaata
      841 ccagctgaat tagggaattt ggttcagctg caagcactcc ggatatacaa gaacaaactt
      901 acttcttcaa ttccatcttc attgttccgg ttaactcagt taacccattt ggggttatca
      961 gaaaaccatt tggttggacc gatatcagaa gaaatcggtt ttcttgagtc acttgaagtc
     1021 ctcacacttc attccaacaa cttcacagga gagtttccac agtccatcac aaacttgagg
     1081 aacttgacag tcctaacggt ggggttcaat aatatttccg gtgagctccc ggcggatcta
     1141 gggcttctta caaaccttcg gaacctttca gcgcacgaca atcttcttac cggaccaata
     1201 ccttccagca taagtaactg caccggtctt aaactcctgg acctgtctca caaccaaatg
     1261 actggcgaga tcccgcgggg tttcggaagg atgaatctta cgttcatttc tattgggagg
     1321 aatcatttca ccggtgaaat tccagatgat atcttcaact gttcaaactt ggaaactctt
     1381 agtgtggcag ataacaactt aacaggaact ctcaagccat taattgggaa gcttcaaaaa
     1441 ctcaggattt tgcaagtttc atataactct ctcactggac cgattcctcg agaaatcggg
     1501 aatctgaaag atttgaatat cttgtacctt cactctaatg gtttcacagg gagaatcccg
     1561 agagagatgt cgaatctcac tctcctccag gggctaagga tgtattcaaa tgatcttgaa
     1621 ggtccaattc ctgaagaaat gtttgatatg aagctactct cagttcttga tctttccaac
     1681 aacaaattct caggtcaaat tcctgccttg ttctccaagc ttgaatcgct tacctacttg
     1741 agtcttcaag gaaacaaatt caacgggtct atccctgcaa gccttaagtc gctttcgctt
     1801 ctcaacacat tcgatatctc cgacaatctt ctcactggaa ccatccctgg agagctgtta
     1861 gcttctttga aaaacatgca gctttacctc aacttctcaa acaacttgtt gactggaacc
     1921 atcccaaagg agcttggaaa gcttgaaatg gttcaagaaa tcgacctttc aaacaatctc
     1981 ttttctgggt ctattccaag atctttacag gcctgcaaaa atgtgttcac actggatttt
     2041 tcgcagaaca atctctcggg tcatatacca gatgaagtct tccaaggcat ggatatgatc
     2101 ataagcttga acctttcaag gaacagtttc tctggagaaa tccctcagag cttcgggaac
     2161 atgacgcatt tggtctcctt ggatctctct agtaacaatc tcactggtga aattccagag
     2221 agtctcgcca atctttcgac tctgaaacat ctcaaactag cttcaaacaa cctcaaaggc
     2281 catgttcctg aatccggggt gttcaaaaac atcaacgcct ctgatctaat gggaaacaca
     2341 gatctctgtg gtagcaagaa gcctctcaag ccatgtacga tcaagcagaa gtcgagccac
     2401 ttctcgaaga gaaccagagt catcctgatt attcttggat cagccgcggc tcttcttctt
     2461 gtcctgcttc ttgttctgat tctaacctgt tgcaagaaaa aagaaaaaaa gattgaaaat
     2521 tcatcagagt cctcattacc ggatttggat tcagctctga aactgaagag atttgaacca
     2581 aaagagttgg agcaagcaac agattcattc aacagtgcca acatcattgg ctcaagcagc
     2641 ttaagcacag tgtacaaagg tcagctagaa gatgggacag tgattgcagt aaaagtattg
     2701 aatctaaagg aattctctgc agaatcagac aagtggttct acacagaagc taaaacattg
     2761 agccaactaa aacatcgaaa cctggtgaag atcttagggt ttgcgtggga aagcggcaaa
     2821 acgaaagctt tagtgcttcc atttatggag aatggaaact tggaggacac cattcacggc
     2881 tctgcagcac cgattgggtc gcttttagaa aaaatcgatc tttgtgttca tatcgcaagc
     2941 ggaatcgatt atcttcattc tggatatggt tttcccatcg ttcattgtga tctgaagcca
     3001 gctaatatac tccttgacag tgaccgcgtt gctcacgtaa gcgattttgg aactgctcgg
     3061 attctaggtt tccgcgaaga tggaagcacc acagcttcaa catcagcctt cgagggtaca
     3121 attggatact tagctccagg taaattactc acaacactta attttcaaaa ccctaattat
     3181 gccatcgact tacttgaaat acactcttat ttttcatgaa cagagtttgc ttatatgagg
     3241 aaagtgacaa caaaagccga tgtattcagc ttcgggatca taatgatgga gctgatgacg
     3301 aaacagagac caacttcgtt gaatgatgaa gattcacaag acatgacttt gcgccaattg
     3361 gtggagaaat cgattggaaa tggaagaaaa gggatggtta gggttcttga tatggaactc
     3421 ggggactcta ttgtttctct gaaacaggaa gaggctattg aagactttct gaagctttgt
     3481 ttgttctgta caagctctag acctgaagat cgacctgata tgaacgagat tcttacacat
     3541 ctgatgaaac ttagaggcaa agcgaattca tttcgagaag atcgtaacga ggatcgagag
     3601 gtgcatatgg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta
     3661 atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga
     3721 taggtcgacc tgcaggcatg cccgctgaaa tcaccagtct ctctctacaa atctatctct
     3781 ctctataata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
     3841 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     3901 tcaataaaat ttctaattcc taaaaccaaa atccaggggt accttaatta agcttggcac
     3961 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4021 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4081 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga
     4141 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
     4201 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     4261 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
     4321 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
     4381 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
     4441 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
     4501 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
     4561 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
     4621 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
     4681 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
     4741 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
     4801 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
     4861 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
     4921 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
     4981 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
     5041 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
     5101 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
     5161 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     5221 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     5281 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
     5341 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     5401 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     5461 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     5521 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     5581 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     5641 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     5701 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     5761 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     5821 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     5881 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     5941 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     6001 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     6061 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     6121 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     6181 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     6241 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     6301 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     6361 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     6421 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     6481 ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc
     6541 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
     6601 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     6661 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     6721 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     6781 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     6841 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     6901 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     6961 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     7021 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     7081 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     7141 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     7201 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     7261 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     7321 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     7381 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     7441 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     7501 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     7561 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     7621 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     7681 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
     7741 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
     7801 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
     7861 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
     7921 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
     7981 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
     8041 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
     8101 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     8161 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     8221 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     8281 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     8341 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     8401 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8461 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8521 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8581 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8641 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8701 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8761 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8821 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8881 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8941 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9001 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9061 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9121 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9181 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact
     9241 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
     9301 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
     9361 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
     9421 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
     9481 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
     9541 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
     9601 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
     9661 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
     9721 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
     9781 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
     9841 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
     9901 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
     9961 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    10021 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    10081 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    10141 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    10201 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
    10261 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
    10321 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
    10381 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    10441 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    10501 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
    10561 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
    10621 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
    10681 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag
    10741 cttgtcgatc gactctagct agaggatcga tccgaacccc agagtcccgc tcagaagaac
    10801 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
    10861 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
    10921 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
    10981 cggccatttt ccaccatgat attcggcaag caggcatcgc catgtgtcac gacgagatcc
    11041 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
    11101 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
    11161 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
    11221 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
    11281 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
    11341 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
    11401 tcctggagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
    11461 tgcgctgaca gccgaaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
    11521 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
    11581 atccccatgg tcgatcgaca gatctgcgaa agctcgagag agatagattt gtagagagag
    11641 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
    11701 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
    11761 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
    11821 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
    11881 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
    11941 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    12001 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    12061 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg
    12121 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    12181 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    12241 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    12301 ctatgacatg attacgaatt cgaagttgtg aattgtgatc ataggagtaa tatttctatc
    12361 tgaacaaaaa ttgaattttg ttttgctttt tgttgaaaga taaataaaat gtttggcaga
    12421 ttgtttgtac agaacacaac ttgttgataa aacaaattcc atcaagacag ctaatgagct
    12481 tttgatagtc agagctttga agacgtgtta atgaattaaa ctgattagac ctaaaaccaa
    12541 atatttagaa agaaaaaaat atgataattg ttatgttatt taatcaatag tcaaatacta
    12601 tgttgagtac caatcatttc agtttcaacg attgctactc ttttgaccaa taacaaaaag
    12661 gtttttattt ttcatttctc aaactccatt tttgtttgct tttagcataa aaacagctag
    12721 aatgtgagtt tcatatgtat tatatactat aagaaatcat ataaattaac gactagaaca
    12781 tattgttttc attctagtgg tatatctgag tttgactaga acgtttggag gcagattaaa
    12841 gtgatttcgc cttgtcaaaa ctactttgta tatgttatca atcaatcgct caaaactaaa
    12901 tcggaaaaaa actacataaa taagatatgg aaaatgatac taacacaaac tagtaagtaa
    12961 gtagtcacta catattctgg tcccatgaat atggcttaca tagtacatac attgtgagat
    13021 taaatgaata tgatatataa tacaagcata taagcatgtg catgactatg aagtgggaat
    13081 acatttagtg cctttatatg caacttgaca gaattaccaa ttatgtgaat agtgaaactt
    13141 atattgcgtc ttggcctttt cacatccgtg acatgatccc tcaaatctat atcttcttgc
    13201 ggcatatgct tatatataaa catatgactt cgtctataaa tttatatcca gataattaat
    13261 tctctcataa tatcttctcc acaatttcaa ctcttctaaa gtctaaaccg gatccgtcta
    13321 aacc
//
