LOCUS       sequence-169786-       11707 bp    DNA     circular SYN 14-APR-2025
DEFINITION  [Edit] Lentiviral vector for Gal4 inducible Blinatumomab and 
            constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 11707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_Blinatumomab_PGK_mCherry
FEATURES             Location/Qualifiers
     source          1..11707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..190
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             343..372
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1867..1884
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1885..1911
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2037..2536
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2072..2095)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2469..2488
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2557..3267
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2702..2720)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2970..2989
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3344..3932
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3397..3417)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3815..3826)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3935..3951)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3936..3952)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4042..4275
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4300..4318)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4301..4318)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4608..4627)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4841..4860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4960..4982
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5020..5038)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5106..5210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5211..5279,5280..6071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5429..5448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6242..6830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6731..6750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6984..7001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7071..7091)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7076..7405
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7256..7391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8539..8604
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8734..8754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9179..9313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9216..9235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9270..9289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9481..10114
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10161..10286
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10783..11016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11201..11245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11530..11647
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggagcactgt cctccgaacg tcggagcact gtcctccgaa cgtcggagca ctgtcctccg
       61 aacgtcggag cactgtcctc cgaacggagc atgtcctccg aacgtcggag cactgtcctc
      121 cgaacgacta gttaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
      181 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      241 ccgatccagc ctctcgacat tcgttggatc gccgccatgg acatgagggt tttggcccag
      301 ctgctggggc tgctgttgct gtgtttcccg ggggccagat gcgagcagaa gctgatcagc
      361 gaggaggacc tggatattca actcacacag tcccccgcgt ctcttgccgt atctttgggc
      421 caacgggcga ccatttcctg caaagcgtcc cagagtgttg attacgatgg ggactcttac
      481 ctcaattggt accagcagat tcccggacag ccgcccaagt tgttgatata tgacgcttca
      541 aacttggtca gtggcattcc tcccagattt tctgggagcg gaagtggtac cgattttaca
      601 cttaacattc atcctgtgga aaaagtggat gccgctactt atcattgtca gcagagtacg
      661 gaagacccct ggacgttcgg tggcggtacg aaactggaga tcaaaggcgg cggtgggagc
      721 ggtgggggtg gatcaggcgg cggcggaagc caagttcaac tgcagcagag tggtgcagag
      781 ctggtcagac ctggcagctc agttaagata tcatgtaaag caagtggata cgcgttttca
      841 agctattgga tgaactgggt caagcaaagg cctggccagg gactggagtg gataggacaa
      901 atttggcccg gggacggtga taccaactac aacggcaagt ttaagggaaa agccaccctg
      961 acggctgatg aatcctcttc tactgcatat atgcaactca gctctttggc aagcgaagac
     1021 agcgccgtgt acttctgcgc caggagggaa actacaactg tgggtcgata ttactacgcg
     1081 atggactact ggggacaggg gacgactgtc actgtttcat caggtggggg aggatcagac
     1141 atcaagctgc aacaatctgg cgctgagttg gcgaggccgg gggcgagtgt taagatgagt
     1201 tgtaagacta gtggttatac tttcaccagg tacaccatgc actgggtgaa gcagcgcccg
     1261 ggacagggtc ttgaatggat aggttatatc aatccgtccc gagggtatac taactacaac
     1321 caaaaattca aagacaaagc gactctgact acggacaaat cctctagcac agcatacatg
     1381 cagctttcca gcctcactag tgaagactca gcagtttact attgtgctag gtactatgat
     1441 gatcactatt gcctcgatta ctggggacaa gggacgaccc tcacggtgtc aagtgtggag
     1501 ggtggctccg gaggttccgg tggatccggc gggagcggtg gagtggatga tatccaactt
     1561 actcaatccc cggccataat gagtgcaagt ccaggagaaa aggtgacaat gacttgccga
     1621 gcgtcatcat ccgtatctta catgaactgg taccagcaga agagtggcac gtctcctaag
     1681 cgatggatat acgacacatc caaggtcgca agcggagtgc cgtaccgatt cagcggttct
     1741 gggtctggga ctagttactc cttgactatc tcatccatgg aagccgaaga cgccgcgaca
     1801 tactattgtc aacaatggtc ctccaacccc cttacgttcg gtgctggaac aaagttggag
     1861 ctgaaacatc accaccatca ccactaccca tacgatgttc cagattacgc ttgagatcct
     1921 tgacttgcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc gcgactctag
     1981 agtcgacctg catctaggcg ccggaattag atctctcgag gttaacgaat tctaccgggt
     2041 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     2101 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     2161 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     2221 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     2281 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     2341 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     2401 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     2461 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     2521 atctccgggc ctttcgacct gcagcccaag cttaccatgg tgagcaaggg cgaggaggat
     2581 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     2641 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2701 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     2761 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     2821 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     2881 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     2941 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     3001 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     3061 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     3121 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     3181 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     3241 ggcggcatgg acgagctgta caagtaaagc ggccgcgact ctagagtcga cctgcaggca
     3301 tgcaagcttg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
     3361 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3421 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3481 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3541 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3601 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3661 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3721 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3781 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3841 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3901 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagggaatta
     3961 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     4021 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4081 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4141 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4201 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4261 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     4321 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
     4381 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
     4441 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
     4501 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
     4561 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     4621 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     4681 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     4741 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     4801 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
     4861 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4921 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4981 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     5041 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     7081 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7141 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7201 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7381 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     7441 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     7501 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     7561 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     7621 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     7681 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     7741 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     7801 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     7861 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     7921 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     7981 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     8041 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     8101 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     8161 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     8221 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     8281 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     8341 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     8401 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
     8461 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     8521 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     8581 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     8641 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     8701 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     8761 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     8821 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     8881 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     8941 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     9001 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     9061 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     9121 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     9181 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9241 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9301 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     9361 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     9421 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     9481 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     9541 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     9601 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     9661 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     9721 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     9781 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     9841 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     9901 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     9961 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10021 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10081 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
    10141 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
    10201 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
    10261 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
    10321 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
    10381 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    10441 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    10501 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    10561 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    10621 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
    10681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
    10741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
    10801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
    10861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
    10921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
    10981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
    11041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
    11101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    11161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    11221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    11281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    11341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    11401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
    11461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
    11521 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    11581 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    11641 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
    11701 cgaattc
//
