LOCUS       sequence-170499-        5980 bp    DNA     circular SYN 14-APR-2025
DEFINITION  A versatile vector for recombinant mycobacterial protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Magana Vergara C, Kallenberg CJL, Rogasch M, Hubner CG, Song YH
  TITLE     A versatile vector for mycobacterial protein production with a 
            functional minimized acetamidase regulon.
  JOURNAL   Protein Sci. 2017 Aug 31. doi: 10.1002/pro.3288.
  PUBMED    28857325
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2017 Aug 31. doi: 10.1002/pro.3288."
COMMENT     Sequence Label: pMyCA
FEATURES             Location/Qualifiers
     source          1..5980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             392..409
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             422..442
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      539..585
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1958..2546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2447..2466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtgcccat gagcgatctc gcgcaggcca cgctgatcac gggtccgact ctcaccagaa
       61 ccgtcgatcg ccttgtgtcg caagggatca tctaccggac tgccgatctg catgaccgcc
      121 ggcgggtgct cgtggcgttg accccgcggg ggcggacgct gcgcaaccgc ctggtggacg
      181 cggtagccga ggccgagtgt gcggcttttg aatcgtgcgg gctggacgtc gaccagttgc
      241 gcgaactcgt cgacaccacc tcgaatttga cttcgtaacc acccgcgccc ggcgcgggcg
      301 ttcacccttg acttttattt tcatctggat atatttcggg tgaatggaaa ggggtgacct
      361 gtacataaga gaaagggagt ccaccatgaa gcaccaccac caccaccacc cgtcggccgg
      421 cgagaacctg tacttccagg gcgccatgga cgataaggta ccggatccga attcgatatc
      481 gctagcaagc ttatcgatgt cgacgtagtt aactagcgta cgatcgactg ccaggcatca
      541 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatgccat catggccgcg
      601 gtgatcagct agagggggcg tcaggcgccg ggggcggtgt ccggcggccc ccagaggaac
      661 tgcgccagtt cctccggatc ggtgaagccg gagagatcca gcggggtctc ctcgaacacc
      721 tcgaagtcgt gcaggaaggt gaaggcgagc agttcgcggg cgaagtcctc ggtccgcttc
      781 cactgcgccc cgtcgagcag cgcggccagg atctcgcggt cgccccggaa ggcgttgaga
      841 tgcagttgca ccaggctgta gcgggagtct cccgcataga cgtcggtgaa gtcgacgatc
      901 ccggtgacct cggtcgcggc caggtccacg aagatgttgg tcccgtgcag gtcgccgtgg
      961 acgaaccggg gttcgcggcc ggccagcagc gtgtccacgt ccggcagcca gtcctccagg
     1021 cggtccagca gccggggcga gaggtagccc cacccgcggt ggtcctcgac ggtcgccgcg
     1081 cggcgttccc gcagcagttc cgggaagacc tcggaatggg gggtgagcac ggtgttcccg
     1141 gtcagcggca ccctgtgcag ccggccgagc acccggccga gttcgcgggc cagggcgagc
     1201 agcgcgttcc ggtcggtcgt gccgtccatc gcggaccgcc aggtggtgcc ggtcatccgg
     1261 ctcatcacca ggtagggcca cggccaggct ccggtgccgg gccgcagctc gccgcggccg
     1321 aggaggcggg gcaccggcac cggggcgtcc gccaggaccg cgtacgcctc cgactccgac
     1381 gcgaggctct ccggaccgca ccagtgctcg ccgaacagct tgatcaccgg gccgggctcg
     1441 ccgaccagta cggggttggt gctctcgccg ggcacccgca gcaccggcgg caccggcagc
     1501 ccgagctcct ccagggctcg gcgggccagc ggctcccaga attcctggtc gttccgcagg
     1561 ctcgcgtagg aatcatccga atcaatacgg tcgagaagta acagggattc ttgtgtcaca
     1621 gcggacctct attcacaggg tacgggccgg cttaattccg cacggccggt cgcgacacgg
     1681 cctgtccgca ccgcggatca ggcgttgacg atgacgggct ggtcggccac gtcggggagt
     1741 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1801 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1861 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgct ctagttccat
     1921 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1981 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2041 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2101 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2161 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2221 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2281 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2341 agcgtgagct atgagaaagc gccacgcttc ccgaggggag aaaggcggac aggtatccgg
     2401 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2461 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2521 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2581 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2641 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2701 acgcgtgcgg ccgcacgcgt gagcccacca gctccgtaag ttcgggcgct gtgtggctcg
     2761 tacccgcgca ttcaggcggc agggggtcta acgggtctaa ggcggcgtgt acggccgcca
     2821 cagcggctct cagcggcccg gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg
     2881 tacaggtggt tgggggtgct cggctgtcgc tggtgttcca ccaccagggc tcgacgggag
     2941 agcgggggag tgtgcagttg tggggtggcc cctcagcgaa atatctgact tggagctcgt
     3001 gtcggaccat acaccggtga ttaatcgtgg tctactacca agcgtgagcc acgtcgccga
     3061 cgaatttgag cagctctggc tgccgtactg gccgctggca agcgacgatc tgctcgaggg
     3121 gatctaccgc caaagccgcg cgtcggccct aggccgccgg tacatcgagg cgaacccaac
     3181 agcgctggca aacctgctgg tcgtggacgt agaccatcca gacgcagcgc tccgagcgct
     3241 cagcgcccgg gggtcccatc cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca
     3301 cgcacacgca gtgtgggcac tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa
     3361 gccgctcgca tacatggcgg cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg
     3421 cagttactca ggcctcatga ccaaaaaccc cggccacatc gcctgggaaa cggaatggct
     3481 ccactcagat ctctacacac tcagccacat cgaggccgag ctcggcgcga acatgccacc
     3541 gccgcgctgg cgtcagcaga ccacgtacaa agcggctccg acgccgctag ggcggaattg
     3601 cgcactgttc gattccgtca ggttgtgggc ctatcgtccc gccctcatgc ggatctacct
     3661 gccgacccgg aacgtggacg gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa
     3721 cgccgaattt ccgtgcaacg acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc
     3781 catcgccaac agcatttggc gttggatcac aaccaagtcg cgcatttggg cggacgggat
     3841 cgtggtctac gaggccacac tcagtgcgcg ccagtcggcc atctcgcgga agggcgcagc
     3901 agcgcgcacg gcggcgagca cagttgcgcg gcgcgcaaag tccgcgtcag ccatgcatgg
     3961 aggcattgct atgagcgacg gctacagcga cggctacaac cggcagccga ctgtccgcaa
     4021 aaagcggcgc gtgaccgccg ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt
     4081 cgtccggctc gtggcgcagg aacgcagcga gtggctcgcc gagcaggctg cacgccgcga
     4141 acgcatccgc gcctatcacg acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt
     4201 cgggctgcat ctggacaccg ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc
     4261 agaacaggaa gcggctcaaa aggcccacaa cgaagccgac aatccaccgc tgttctaacg
     4321 caattgggga gcgggtgtcg cgggggttcc gtggggggtt ccgttgcaac gggtcggaca
     4381 ggtaaaagtc ctggtagacg ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt
     4441 ttcgttgcgc ccgttttgaa taccagccag acgagacggg gttctacgaa tcttggtcga
     4501 taccaagcca tttccgctga atatcgggga gctcaccgcc agaatcggtg gttgtggtga
     4561 tgtacgtggc gaactccgtt gtagtgcctg tggtggcatc cgtggcgcgg ccgcggtacc
     4621 agatctttaa atctagataa agaagtgacg cggtctcaag cgtcgagcgt cgccagcgtg
     4681 tcgaggatgt cgaagtcgta gccgtcggcg ctggcgatgt agacctgctg gtcgaattga
     4741 ctgtcgcgca tacacatcgg gccccggggc ccgtcgaacc cgacatcgtg cgcggatgcc
     4801 atcaggtccg gtatctcggg ggagtgggcc cgctggaaga tggcctcgag cgcaagcaga
     4861 ccctcgtaac aggattcggc catcgcgttg agcggtggcg cgtcggcgcc gtagcgggcg
     4921 acgtagctgc ccatcaggtc catcgcaccc gcggtggcca gtgaactgaa gtacgccgcg
     4981 gcgacataga ggttttcggt ggagccggcg ccgctggcca gcagcatgtt ctcctccatc
     5041 agcgggctga accgcgccat gcggtcgtgc ccgccggcgc gcgcgaactc gcggttgaac
     5101 aacacggcgt cctggccgac gagcagcatc aacacggcct gcgcccccga cgcgatggcc
     5161 ttgcggacag gtgcgcggaa atcgtcggtg ccgtacggga cgtagatctc ccgtctgagc
     5221 tcgaggtcca gatctcggca gtacgcgcgg gcggccgcgg cggaacggcg cggccagatg
     5281 tagtcatcgc cgaccaggca ccaggaccgg atgccgaagt ggtcgcgcag ccaggcgagc
     5341 gcgggcgcga tctggatctg cggtgtctcg cctgtgcaga acacgcccgg tgtgcgttca
     5401 ccgccctcgt acaacgaggt gtagacgtac gggatgcggt cgcggaccac cggggagatg
     5461 cggttgcgca cggccgagat gtgccagccg gtcacggcgt cgagaccgtg acctcgcaac
     5521 cggtcggcga cggtccgggc gacgtcgtcg ccgggcgctc cgccgtcgag cacctcgatg
     5581 gtgaccttgc ggccctgcag gccgcctcgg tcgttgacct ccttggccgc gagctcggcc
     5641 acggcctcgc acgaaggcgc gaagattccc gctggccctt gaagcggaat caccagcccg
     5701 acgcggaact caacctcgcc gtcctgcact ccagatcacc gtcgatcccg tgtagtctgc
     5761 gcttcaaagg tttctagcag aaataattca ttctgaacag accccgccgt cgacacgagg
     5821 agacccaaca tggccgccgg acagcagcgc cgccccaacc tcctgctgcc gttggtgcgt
     5881 ctgacccacc tcgcggagtc ggcgatcgaa cgcgtgctcg cggactcgtc gctcaagatc
     5941 gaggactggc gcgtgctcga cgagttggcc ggacggcgca
//
