LOCUS       sequence-170536-        9749 bp    DNA     circular SYN 14-APR-2025
DEFINITION  C-terminal flag-tagged protein expression in mammalian cells, 
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Yun YS, Kim KH, Tschida B, Sachs Z, Noble-Orcutt KE, Moriarity BS, 
            Ai T, Ding R, Williams J, Chen L, Largaespada D, Kim DH
  TITLE     mTORC1 Coordinates Protein Synthesis and Immunoproteasome Formation 
            via PRAS40 to Prevent Accumulation of Protein Stress.
  JOURNAL   Mol Cell. 2016 Feb 18;61(4):625-39. doi: 
            10.1016/j.molcel.2016.01.013. Epub 2016 Feb 11.
  PUBMED    26876939
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.01.013"; journalName: "Mol Cell"; date: 
            "2016-02-18- 18"; volume: "61"; issue: "4"; pages: "625-39"
COMMENT     Sequence Label: pcsII-pb2-flag
FEATURES             Location/Qualifiers
     source          1..9749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1442..1675
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1860..1904
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2178..2295
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2351..3513
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2582..3504
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3461..3481
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4137..4160
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4210..4798
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4263..4283)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4681..4692)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5317..5497
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5523..5540)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5529..5753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5799..6227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5886..5905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6096..6117
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6236..6256)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6241..6570
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6421..6556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6483..6502
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6618..6665
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6684..7058
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7188..7309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7225..7244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7279..7298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7358..7374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7358..7374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7371..7393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7382..7398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7406..7412,7413..7430,7431..7436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7451..7472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7589..7606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7760..8348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7840..7859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8519..9310,9311..9379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9142..9161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9380..9484)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9559..9578)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagggc cgctgatctt cagacctgga ggaggagata
     1321 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1381 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1441 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1501 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1561 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1621 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1681 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1741 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1801 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1861 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1921 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1981 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2041 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2101 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctacaaatg
     2161 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2221 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2281 aaaattcaaa attttcgggt ttattacagg gacagcagaa attcactttg aattaattca
     2341 agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2401 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     2461 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     2521 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2581 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     2641 gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg
     2701 ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     2761 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     2821 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     2881 agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc
     2941 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     3001 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     3061 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     3121 tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg
     3181 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca
     3241 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     3301 tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     3361 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     3421 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     3481 caaagttttt ttcttccatt tcaggtgtcg tgaacacgct accggtctcg accatggagt
     3541 acctcatcgg tatccaaggc cccgactatg ttcttgtcgc ctccgaccgg gtggccgcca
     3601 gcaatattgt ccagatgaag gacgatcatg acaagatgtt taagatgagt gaaaagatat
     3661 tactcctgtg tgttggagag gctggagaca ctgtacagtt tgcagaatat attcagaaaa
     3721 acgtgcaact ttataagatg cgaaatggat atgaattgtc tcccacggca gcagctaact
     3781 tcacacgccg aaacctggct gactgtcttc ggagtcggac cccatatcat gtgaacctcc
     3841 tcctggctgg ctatgatgag catgaagggc cagcgctgta ttacatggac tacctggcag
     3901 ccttggccaa ggcccctttt gcagcccacg gctatggtgc cttcctgact ctcagtatcc
     3961 tcgaccgata ctacacaccg actatctcac gtgagagggc agtggaactc cttaggaaat
     4021 gtctggagga gctccagaaa cgcttcatcc tgaatctgcc aaccttcagt gttcgaatca
     4081 ttgacaaaaa tggcatccat gacctggata acatttcctt ccccaaacag ggctccgact
     4141 acaaggacga cgatgacaag gcggccgcgg atcctctaga gttaacatcg agggatcaag
     4201 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4261 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4321 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4381 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4441 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4501 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4561 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4621 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4681 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4741 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     4801 cgataccgtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     4861 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     4921 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4981 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     5041 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     5101 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     5161 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     5221 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     5281 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     5341 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5401 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5461 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     5521 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5581 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5641 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5701 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5761 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5821 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5881 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5941 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6001 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6061 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6121 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6181 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6241 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6301 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6361 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6421 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6481 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6541 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6601 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     6661 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     6721 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     6781 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     6841 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     6901 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     6961 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     7021 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     7081 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7141 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7321 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7381 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7441 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7501 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7561 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8161 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8401 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8461 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8521 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8581 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8641 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8701 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8761 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8821 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8881 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8941 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9001 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9061 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9121 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9181 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9241 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9301 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9361 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9421 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9481 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     9541 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     9601 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     9661 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     9721 tcgcgatgta cgggccagat atacgcgtt
//
