LOCUS       sequence-17079-e         796 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Bastians H, Topper LM, Gorbsky GL, Ruderman JV
  TITLE     Cell cycle-regulated proteolysis of mitotic target proteins.
  JOURNAL   Mol Biol Cell 1999 Nov;10(11):3927-41.
  PUBMED    10564281
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "1999-11"; volume: "10(11)"; pages: "3927-41"
COMMENT     Sequence Label: pET-UbcH10-wt
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             24..46
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             597..614
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gnnnnnnntc cctctagaat aattttgttt aactttaaga aggagatata catatggctt
       61 cccaaaaccg cgacccagcc gccactagcg tcgccgccgc ccgtaaagga gctgagccga
      121 gcgggggcgc cgcccggggt ccggtgggca aaaggctaca gcaggagctg atgaccctca
      181 tgatgtctgg cgataaaggg atttctgcct tccctgaatc agacaacctt ttcaaatggg
      241 tagggaccat ccatggagca gctggaacag tatatgaaga cctgaggtat aagctctcgc
      301 tagagttccc cagtggctac ccttacaatg cgcccacagt gaagttcctc acgccctgct
      361 atcaccccaa cgtggacacc cagggtaaca tatgcctgga catcctgaag gaaaagtggt
      421 ctgccctgta tgatgtcagg accattctgc tctccatcca gagccttcta ggagaaccca
      481 acattgatag tcccttgaac acacatgctg ccgagctctg gaaaaacccc acagctttta
      541 agaagtacct gcaagaaacc tactcaaagc aggtcaccag ccaggagccc ctcgagcacc
      601 accaccacca ccactgannc gngncancnn aacaaacccc gaaaggaagc tgaagttggc
      661 tgctgccacc gctgnaacaa taactagcat aaccccttgg ggcnnnnnna cgggtcttga
      721 ggggtttttt gctgaaggag gaactatatc cggattggng aatgggacgc gccctgtagc
      781 ggcgcattaa gcgcgg
//
