LOCUS       sequence-170980-       13515 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentivirus vector. Expresses H2A-Halotag fusion proteins in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13515)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-H2A-HT
FEATURES             Location/Qualifiers
     source          1..13515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     135..154
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             316..918
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     815..835
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    934..1522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(987..1007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1405..1416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1729..1962
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2063..2080)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2069..2293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2339..2767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2426..2445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2636..2657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2776..2796)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2781..3110
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2961..3096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3023..3042
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    4318..4439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4488..4504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4488..4504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4501..4523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4512..4528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4536..4542,4543..4560,4561..4566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4581..4602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4719..4736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4890..5478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4970..4989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5649..6440,6441..6509))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6272..6291
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6510..6614)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6662..6796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6964..7597
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7644..7769
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8266..8499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8684..8728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9026..9143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9208..9582)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      9579..9588
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9592..9610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9602..9649)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    9685..9703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9720..9738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9756..9774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9792..9810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9827..9845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9863..9881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        9893..9931
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9928..9952
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10394..11284
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     promoter        11296..12507
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     12344..12363
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             12526..13233
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    join(13256..13515,1..313)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(13423..13440)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
       61 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
      121 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
      181 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
      241 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      301 aaacacgata ataccatggc caccgagtac aagcccacgg tgcgcctcgc cacccgcgac
      361 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
      421 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
      481 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
      541 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggctcgcgc
      601 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
      661 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
      721 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgct
      781 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
      841 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
      901 cgcaagcccg gtgcctgagt tcgcgtctgg aacaatcaac ctctggatta caaaatttgt
      961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1021 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     1081 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     1141 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     1201 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     1261 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     1321 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     1381 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     1441 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     1501 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     1561 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     1621 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     1681 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     1741 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     1801 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1861 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1921 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     1981 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     2041 attgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2101 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2161 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2221 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     2281 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     2341 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2401 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2461 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2521 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2581 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2641 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2701 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2761 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2821 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2881 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2941 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3001 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     3061 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     3121 gagcttgtat atccattttc ggatctgatc agcacgtgat gaaaaagcct gaactcaccg
     3181 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     3241 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     3301 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     3361 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattaatt cagcgagagc
     3421 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     3481 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     3541 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     3601 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     3661 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     3721 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     3781 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     3841 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     3901 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     3961 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     4021 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     4081 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     4141 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtgctacga
     4201 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4261 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4321 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4381 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4441 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     4501 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4561 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4621 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4681 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4741 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4801 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4861 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4921 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4981 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5041 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5101 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5161 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5221 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5281 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5341 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5401 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5461 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5521 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5581 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5641 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5701 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5761 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5821 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5881 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5941 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6001 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6061 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6121 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6181 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6241 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6301 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6361 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6421 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6481 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6541 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6601 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     6661 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6721 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6781 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     6841 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     6901 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     6961 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     7021 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     7081 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     7141 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     7201 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     7261 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     7321 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     7381 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     7441 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7501 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7561 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7621 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7681 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7741 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     7801 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     7861 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7921 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7981 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     8041 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8101 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8161 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8221 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8281 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8341 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8401 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8461 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8521 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8581 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     8641 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     8701 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     8761 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     8821 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     8881 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     8941 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     9001 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9061 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9121 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     9181 tagtaccggg cccgctctag tccggaatca gtcctgctcc tcggccacga agtgcacgca
     9241 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
     9301 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
     9361 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
     9421 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
     9481 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
     9541 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtggccc tcctatagtg
     9601 agtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca cgtgctgcag
     9661 gtccgaggtt ctagacgagt ttactcccta tcagtgatag agaacgatgt cgagtttact
     9721 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     9781 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat
     9841 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg
     9901 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcaccggt
     9961 ccgctcgagg gccaccatgt ctggacgtgg caagcaggga ggcaaagccc gcgctaaggc
    10021 caagactcgc tcttctaggg ccggtctcca gttccccgtg ggccgagtgc accgcctgct
    10081 ccgcaaaggc aactatgccg agcgggtcgg ggccggcgcg ccggtgtatc tggcagcggt
    10141 gctggagtac ctgaccgccg agatcctgga actggcgggc aacgcggccc gcgacaacaa
    10201 gaagacccgc atcatcccgc gtcatctcca actggccatc cgcaacgacg aggagctcaa
    10261 caagctgctg ggcaaagtca ccatcgcaca gggcggtgtc ctgcccaaca ttcaggccgt
    10321 gctactgccc aaaaagactg agagccacca caaggcgaag ggcaagtcgg cgaattcgat
    10381 cgatcctgca ggaatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga
    10441 agtcctgggc gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct
    10501 gttcctgcac ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc
    10561 accgacccat cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga
    10621 cctgggttat ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg
    10681 tctggaagag gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc
    10741 caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc
    10801 gacctgggac gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga
    10861 cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg
    10921 tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc
    10981 tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc
    11041 gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa
    11101 gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc
    11161 caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga
    11221 agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc
    11281 cggctgagca cgcgtggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
    11341 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
    11401 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
    11461 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
    11521 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
    11581 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
    11641 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
    11701 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
    11761 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
    11821 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
    11881 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
    11941 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
    12001 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
    12061 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
    12121 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
    12181 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
    12241 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
    12301 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
    12361 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
    12421 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
    12481 tggccgtttt tggctttttt gttagacgct agcggatccg ccaccatgtc taggctggac
    12541 aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg
    12601 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
    12661 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
    12721 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
    12781 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
    12841 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
    12901 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
    12961 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
    13021 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
    13081 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
    13141 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
    13201 gacgattttg accttgacat gctccccggg taaggatccg cggccgcaaa ttccgcccct
    13261 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
    13321 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
    13381 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
    13441 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
    13501 cgtctgtagc gaccc
//
