LOCUS       sequence-170982-       13303 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentivirus vector. Expresess mutant Halotag-CD-Cbx7 fusion proteins 
            in mammalian cells. Express Chromodomain of Cbx7; amino acids 8–62..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13303)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-HT-CD(Cbx7)
FEATURES             Location/Qualifiers
     source          1..13303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    102..674
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(269..286)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     496..515
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             677..1279
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1176..1196
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1295..1883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1348..1368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1766..1777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2090..2323
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2424..2441)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2430..2654
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2700..3128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2787..2806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2997..3018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3137..3157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3142..3471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3322..3457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3384..3403
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    4679..4800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4849..4865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4849..4865)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4862..4884)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4873..4889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4897..4903,4904..4921,4922..4927))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4942..4963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5080..5097)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5251..5838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5330..5349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6009..6800,6801..6869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6632..6651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6870..6974)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7022..7156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             7324..7957
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8004..8129
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8626..8859
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9044..9088
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9386..9503
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9568..9942)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      9939..9948
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9952..9970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9962..10009)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    10045..10063
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10080..10098
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10116..10134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10152..10170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10187..10205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10223..10241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        10253..10291
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10288..10312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      10331..10340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10337..11227
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     promoter        11445..12656
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     12493..12512
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(12675..13303,1..79)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
ORIGIN
        1 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg attttgacct
       61 tgacatgctc cccgggtaag gatccgcggc cgcaaattcc gcccctctcc ctcccccccc
      121 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
      181 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
      241 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
      301 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
      361 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
      421 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
      481 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
      541 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
      601 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
      661 aaaacacgat aataccatgg ccaccgagta caagcccacg gtgcgcctcg ccacccgcga
      721 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
      781 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
      841 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
      901 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg
      961 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     1021 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     1081 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     1141 tggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     1201 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     1261 ccgcaagccc ggtgcctgag ttcgcgtctg gaacaatcaa cctctggatt acaaaatttg
     1321 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1381 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1441 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     1501 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     1561 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     1621 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     1681 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     1741 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     1801 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     1861 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     1921 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     1981 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     2041 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     2101 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     2161 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2221 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2281 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     2341 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     2401 cattgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     2461 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     2521 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     2581 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     2641 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     2701 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2761 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2821 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2881 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     2941 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3001 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3061 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3121 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     3481 ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc tgaactcacc
     3541 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     3601 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     3661 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     3721 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattaat tcagcgagag
     3781 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     3841 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     3901 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     3961 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     4021 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     4081 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     4141 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     4201 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     4261 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     4321 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     4381 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     4441 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     4501 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtgctacg
     4561 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     4621 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     4681 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4741 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4801 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     4861 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4921 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4981 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5041 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5101 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5161 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5221 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5281 tcacaaaaat cgacgctcaa gtcagagggg cgaaacccga caggactata aagataccag
     5341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5401 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5941 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6001 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6061 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6121 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6181 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6241 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6301 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6361 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6421 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6481 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6541 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6601 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6661 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6721 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6781 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6841 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6961 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     7021 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7141 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     7201 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     7261 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     7321 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     7381 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     7441 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     7501 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     7561 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     7621 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     7681 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     7741 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     7801 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7861 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7921 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7981 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     8041 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     8101 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8161 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8221 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     8281 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     8341 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     8401 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8461 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8521 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8581 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8641 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8701 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8761 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8821 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8881 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8941 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9001 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9061 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9121 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9181 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9241 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     9301 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     9361 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9421 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9481 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     9541 tagtaccggg cccgctctag tccggaatca gtcctgctcc tcggccacga agtgcacgca
     9601 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
     9661 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
     9721 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
     9781 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
     9841 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
     9901 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtggccc tcctatagtg
     9961 agtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca cgtgctgcag
    10021 gtccgaggtt ctagacgagt ttactcccta tcagtgatag agaacgatgt cgagtttact
    10081 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
    10141 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat
    10201 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg
    10261 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcaccggt
    10321 ccgctcgagg gccaccatgg cagaaatcgg tactggcttt ccattcgacc cccattatgt
    10381 ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt ccgcgcgatg gcacccctgt
    10441 gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg cgcaacatca tcccgcatgt
    10501 tgcaccgacc catcgctgca ttgctccaga cctgatcggt atgggcaaat ccgacaaacc
    10561 agacctgggt tatttcttcg acgaccacgt ccgcttcatg gatgccttca tcgaagccct
    10621 gggtctggaa gaggtcgtcc tggtcattca cgactggggc tccgctctgg gtttccactg
    10681 ggccaagcgc aatccagagc gcgtcaaagg tattgcattt atggagttca tccgccctat
    10741 cccgacctgg gacgaatggc cagaatttgc ccgcgagacc ttccaggcct tccgcaccac
    10801 cgacgtcggc cgcaagctga tcatcgatca gaacgttttt atcgagggta cgctgccgat
    10861 gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat taccgcgagc cgttcctgaa
    10921 tcctgttgac cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg ccggtgagcc
    10981 agcgaacatc gtcgcgctgg tcgaagaata catggactgg ctgcaccagt cccctgtccc
    11041 gaagctgctg ttctggggca ccccaggcgt tctgatccca ccggccgaag ccgctcgcct
    11101 ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc ccgggtctga atctgctgca
    11161 agaagacaac ccggacctga tcggcagcga gatcgcgcgc tggctgtcga cgctcgagat
    11221 ttccggcgcg aattctggcg gttcgggtgg aggcgggagt ggaggtgagc aggtgttcgc
    11281 cgtggagagc atccggaaga agcgcgtgcg gaagggtaaa gtcgagtatc tggtgaagtg
    11341 gaaaggatgg cccccaaagt acagcacgtg ggagccagaa gagcacatct tggacccccg
    11401 cctcgtcatg gcctacgagg agaaggagga gtgagcggac gcgtggcctc cgcgccgggt
    11461 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
    11521 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
    11581 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
    11641 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
    11701 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
    11761 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
    11821 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
    11881 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
    11941 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
    12001 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
    12061 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
    12121 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
    12181 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
    12241 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
    12301 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
    12361 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
    12421 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
    12481 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
    12541 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
    12601 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgcta
    12661 gcggatccgc caccatgtct aggctggaca agagcaaagt cataaacgga gctctggaat
    12721 tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
    12781 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc
    12841 caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc
    12901 aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg
    12961 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
    13021 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
    13081 ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag
    13141 aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc
    13201 tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
    13261 gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cga
//
