LOCUS       sequence-171408-       10160 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  P. falciparum integration plasmid for N-terminal gene fusions 
            (double 2xFKBP) and placing loxP sites using SLI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, 
            Jonscher E, Bergmann B, Spielmann T
  TITLE     A genetic system to study Plasmodium falciparum protein function.
  JOURNAL   Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.
  PUBMED    28288121
REFERENCE   2  (bases 1 to 10160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223."
COMMENT     Sequence Label: pSLI-N-sandwich-loxP(K13)
FEATURES             Location/Qualifiers
     source          1..10160
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1267..1286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1268..1286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1293..1310)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1293..1309)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1302..1324)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1518..1537)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1637..1659
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1697..1715)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1783..1887
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1888..1956,1957..2748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2106..2125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2919..3507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3408..3427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3661..3678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3795..3816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3831..3836,3837..3854,3855..3861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3869..3885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3874..3896
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3893..3909
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3893..3909
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3927..3945
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3927..3944
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4521..4550
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4554..4583
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(4584..4617)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4623..4676
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5622..5675
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5685..6005
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6051..6371
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6420..7133
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6420..7133
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6450..6478)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7073..7093
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             7206..7526
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             7533..7853
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     protein_bind    complement(10095..10128)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atatatatat atatttataa tatgtatatt ttaaactaga aaaggaataa ctaatatttt
       61 atttattatc attcaagatt tatattttat aataataaat acctaataga aatatatcag
      121 gatccatgca tggttcgcta aactgcatcg ttgctgtgtc ccagaacatg ggcatcggca
      181 agaacgggga ctacccctgg ccaccgctca ggaacgaatt tagatatttc cagagaatga
      241 ccacaacctc ttcagtagaa ggtaaacaga atctggtgat tatgggtaag aagacctggt
      301 tctccattcc tgagaagaat cgacctttaa agggtagaat taatttagtt ctcagcagag
      361 aactcaagga acctccacaa ggagctcatt ttctttccag aagtctagat gatgccttaa
      421 aacttactga acaaccagaa ttagcaaata aagtagacat ggtctggata gttggtggca
      481 gttctgttta taaggaagcc atgaatcacc caggccatct taaactattt gtgacaagga
      541 tcatgcaaga ctttgaaagt gacacgtttt ttccagaaat tgatttggag aaatataaac
      601 ttctgccaga atacccaggt gttctctctg atgtccagga ggagaaaggc attaagtaca
      661 aatttgaagt atatgagaag aatgattaag cttatttaat aatagattaa aaatattata
      721 aaaataaaaa cataaacaca gaaattacaa aaaaaataca tatgaatttt tttttgtaat
      781 cttccttata aatatagaat aatgaatcat ataaaacata tcattattca tttatttaca
      841 tttaaaatta ttgtttcagt atctttaatt tattatgtat atataaaaat aacttacaat
      901 tttattaata agcaatatat gtttattaat tcatgttttg taatttatgg gatagcgatt
      961 ttttttactg tctgtatttt tcttttttaa ttatgtttta attgtatttt atttttatta
     1021 ttgttctttt tatagtatta ttttaaaaca aaatgtattt tctaagaact tataataata
     1081 ataaatataa attttaataa aaattatatt tatcttttac aatatgaaca taaagtacaa
     1141 cattaatata tagcttttaa tatttttatt cctaatcatg taaatcttaa atttttcttt
     1201 ttaaacatat gttaaatatt tatttctcat tatatataag aacatattta ttaaatctag
     1261 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     1321 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1381 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1441 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1501 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     1561 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     1621 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     1681 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1741 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1801 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1861 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1921 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1981 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2041 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2101 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2161 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2221 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2281 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2341 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2401 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2461 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2521 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2581 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2641 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2701 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2761 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2821 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2881 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2941 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3001 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3061 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3121 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3181 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3241 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3301 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3361 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3421 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3481 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3541 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3601 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3661 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3721 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3781 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     3841 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     3901 agctatgacc atgattacgc caagctattt aggtgacact atagaatact caagctgcgg
     3961 ccgctaaaga tgcagcaaat cttataaatg atgattctgg attaaacaat ttaaaaaaat
     4021 tttcaactgt aaataatgta aatgatactt atgaaaagaa aattattgaa acggaattaa
     4081 gtgatgctag tgattttgaa aatatggtag gtgatttaag aattacattt attaattggt
     4141 taaaaaagac acaaatgaat tttattcgag aaaaagataa attatttaaa gataagaaag
     4201 aactagaaat ggaaagagta cgattgtaca aagaattaga aaaccgtaaa aatattgaag
     4261 aacagaaatt acatgatgaa agaaagaaat tagatattga tatatctaat ggttataaac
     4321 aaataaaaaa agaaaaagaa gaacatagga aacgatttga tgaagaaaga ttaagatttt
     4381 tacaagaaat cgataaaatt aaattagtat tatatttaga aaaagaaaaa tattatcaag
     4441 aatataaaaa ttttgagaat gataaaaaaa aaattgttga tgcaaatatt gctactgaaa
     4501 ctatgattga tggtttaaac gagcagaagt taatatcaga agaggatttg ggtgaacaaa
     4561 aactcataag cgaagaagat ttaataactt cgtatagcat acattatacg aagttatccg
     4621 gagaaggaag aggaagttta ttaacatgtg gagatgtaga agaaaatcca ggaccaatga
     4681 cagccagttt aactaccaag ttcttgaaca atacctatga aaacccattt atgaatgcat
     4741 ccggtgttca ttgcatgact acacaagaat tagatgaatt agcaaactct aaagctggcg
     4801 cattcattac aaagagtgct acaaccttag aaagagaagg taaccctgaa ccacgttaca
     4861 tttctgtccc tctaggcagt atcaactcca tgggtttacc aaacgaaggt atcgactact
     4921 atttgtccta tgtattaaac cgtcaaaaga attatcctga tgcacctgct attttcttct
     4981 cagttgctgg tatgagcatt gatgaaaatt taaatttgtt gaggaaaatc caagatagcg
     5041 aattcaacgg tattaccgag ttaaacttgt cttgtcctaa tgtgcctggg aaaccacaag
     5101 ttgcttatga ctttgacttg acaaaggaaa ccttggaaaa ggtttttgcc tttttcaaaa
     5161 aacctcttgg tgtcaagttg cctccttatt ttgattttgc ccattttgat atcatggcaa
     5221 aaatattgaa cgagttccca ttagcttatg tcaactctat caatagtata ggaaatggtc
     5281 ttttcattga tgtggagaag gagagtgtag tagtgaagcc aaagaatggt ttcgggggta
     5341 ttggaggtga atatgttaag ccaaccgcgc tcgccaatgt tcgtgcattt tacactcgtt
     5401 tgagacctga aatcaaagtt atcggtacag gtggaattaa gtccggtaag gatgcatttg
     5461 aacatcttct atgtggtgcc tctatgctac agattggtac agaattacaa aaagagggcg
     5521 tcaagatttt tgaacgtatc gaaaaagaat taaaagacat aatggaagct aagggttata
     5581 catccataga tcagttccgt gggaagttga acagcattgg tgaaggtaga ggttctttgt
     5641 tgacttgtgg tgatgttgaa gaaaatccag gtccagctag ccgtggtgtt caggtcgaga
     5701 ctattagccc tggagatgga cgcacgtttc ctaagcgtgg acagacatgc gtagttcact
     5761 acacaggtat gttggaggac ggtaaaaagt tcgacagctc acgcgaccgc aataaacctt
     5821 tcaagtttat gcttggcaag caggaggtta ttcgtggatg ggaggagggt gtagcacaga
     5881 tgtctgttgg acagcgtgct aagttgacaa tttcacctga ctatgcttat ggcgctacgg
     5941 gccatcccgg gatcattccg ccacatgcga ctctggtatt cgacgttgaa ttattaaagt
     6001 tagagacagc tagaggggcc gctgcaggtg ctggtggagc tggaagacgt ggagtacaag
     6061 tagagactat ctctccaggt gacggtcgca ctttcccaaa gcgtggccaa acctgtgttg
     6121 tacattacac tggtatgctg gaggatggga aaaagttcga ttccagtcgc gaccgtaaca
     6181 aaccgttcaa attcatgttg ggaaagcagg aagtgatccg cgggtgggag gaaggcgtgg
     6241 cgcaaatgag cgtcggtcag cgggctaaat tgaccatttc ccctgactac gcgtatgggg
     6301 ctactgggca cccagggatt attccgcctc acgctacact tgtgtttgat gtcgaacttt
     6361 tgaaactgga aactgccagg ggagcagccg caggagcagg gggggcagga agggtcgaca
     6421 tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     6481 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     6541 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     6601 tcactacttt cgcgtatggt cttcaatgct ttgcgagata cccagatcat atgaaacagc
     6661 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     6721 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     6781 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     6841 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     6901 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     6961 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     7021 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     7081 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaacctagct
     7141 caggattgag atcaagatct gctgctgctg gtgctggtgg tgctgctaga gctgctctgc
     7201 agagaggagt acaagttgaa acaatatcac caggagatgg tcgtacattt ccaaaaagag
     7261 gtcaaacttg tgttgtacat tatactggaa tgcttgaaga tggaaagaaa tttgattcat
     7321 ctcgtgatag aaataaacca tttaaattta tgctaggtaa acaagaagta atacgaggtt
     7381 gggaagaagg agttgctcaa atgagtgtag gtcaaagagc aaaacttact atatctccag
     7441 attatgctta tggtgcaact ggacatccag gtataattcc acctcatgca actcttgtat
     7501 ttgatgtgga gcttctaaaa ctagaaacta gaggtgttca ggttgaaaca atttcacctg
     7561 gagatggcag aacctttcct aaaagaggac agacttgcgt agttcattat acaggcatgc
     7621 tagaggatgg taagaaattt gattctagtc gagatagaaa taagccattc aagtttatgc
     7681 taggtaaaca ggaagtaata agaggttggg aagagggtgt agcacagatg tcagttggac
     7741 aaagagcaaa gttaacaata tcaccagatt atgcatacgg tgcaacaggc catcctggca
     7801 tcatccctcc acatgcaact ttagtattcg acgttgaatt gttaaagtta gagacaacgc
     7861 gtgctagagg tgctgctgct ggtgctggag gtgcaggtag acctaggatg gagggtgaga
     7921 aggttaagac taaagctaac agtataagta acttcagtat gacttacgac agagagagtg
     7981 gagggaatag taactcagac gacaagagtg gttcaagttc agaaaacgac tcaaacagtt
     8041 ttatgaactt aacatctgac aagaacgaaa agacagagaa caactcattt ttacttaaca
     8101 actcatctta cggtaacgta aaggactctt tattggagtc tatagacatg tcagtgttgg
     8161 acagtaattt cgactcaaag aaggacttct tgccttcaaa cttgtctagg actttcaaca
     8221 acatgagtaa ggacaacatt ggtaacaagt acttgaacaa gttattgaac aagaagaagg
     8281 acacaataac taacgagaac aacaacataa accacaacaa caacaacaac aacttaactg
     8341 ctaacaacat tacaaacaac ttaataaaca acaacatgaa ctcaccttct ataatgaaca
     8401 caaataagaa ggaaaacttc ttggacgctg ctaacttgat taacgacgac tcaggtttga
     8461 ataacttgaa gaagttctct acagttaaca acgttaacga cacatacgag aaaaagataa
     8521 tagagaccga gttgtcagac gcatcagact tcgagaacat ggttggagac ttgaggataa
     8581 ctttcataaa ctggttgaag aaaactcaga tgaacttcat aagagagaag gacaagttgt
     8641 tcaaggacaa aaaggagctt gagatggaga gggttcgttt atataaggag ttggagaatc
     8701 gtaagaacat agaggagcaa aagttgcacg acgagaggaa aaagttggac atagacattt
     8761 caaacggata caagcagatt aagaaggaga aggaggagca cagaaagcgt ttcgacgagg
     8821 agaggttgag gttcttgcag gagatagaca agataaagtt ggttttgtac ttggagaagg
     8881 agaagtacta ccaggagtac aagaacttcg aaaacgacaa gaagaagata gtagacgcta
     8941 acatagcaac agagacaatg atagacataa acgtaggagg tgcaatattc gagacttcaa
     9001 ggcacacttt gactcagcag aaggactctt tcattgaaaa gttgttgtca ggtaggcacc
     9061 acgttacaag ggacaagcag ggtaggattt ttttggacag agactcagaa ttgttcagga
     9121 taattttaaa tttcttgagg aaccccttga caattcctat tcctaaggac ttgtctgagt
     9181 cagaggcttt attaaaggag gctgagttct acggaataaa gttcttgcct tttcctttgg
     9241 ttttctgcat tggaggtttc gacggagttg agtacttgaa cagtatggag ttgttggaca
     9301 tatcacagca gtgttggcga atgtgcactc caatgtcaac taagaaggca tacttcggtt
     9361 ctgcagtttt aaacaacttt ttgtatgtat tcggaggaaa caattacgac tacaaagcat
     9421 tgttcgagac agaggtatac gacaggttga gggacgtttg gtacgtaagt tcaaacttga
     9481 acattccaag gaggaacaac tgcggagtaa cctctaacgg aaggatatac tgcataggag
     9541 gttacgacgg atcatcaata attcccaacg ttgaggctta cgaccacaga atgaaggctt
     9601 gggttgaagt agctccatta aacactccaa ggtctagtgc aatgtgcgta gcattcgaca
     9661 acaagatata cgtgatagga ggtacaaacg gagaaaggtt gaactcaata gaggtttacg
     9721 aggagaagat gaacaagtgg gagcagttcc cttacgcatt gttggaggca aggtcaagtg
     9781 gtgctgcatt caactattta aaccagattt acgtagtagg tggaatagac aacgagcaca
     9841 atattttgga ctctgtagag cagtaccagc ctttcaacaa gaggtggcag ttccttaacg
     9901 gagttcctga aaagaagatg aacttcggtg cagctacttt aagtgactca tacattataa
     9961 ctggtggtga gaacggggag gtacttaact cttgccactt tttctcacct gacactaacg
    10021 agtggcaatt aggaccttca ttgttggtac caaggttcgg acattcagta ttgattgcta
    10081 acatttgaag gcctataact tcgtatagca tacattatac gaagttatta tgactcgagg
    10141 atatggcagc ttaatgttcg
//
