LOCUS       sequence-171485-       13673 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentivirus for expression of human Plexin-B2 with mutant GAP domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Huang Y, Tejero R, Lee VK, Brusco C, Hannah T, Bertucci TB, 
            Junqueira Alves C, Katsyv I, Kluge M, Foty R, Zhang B, Friedel CC, 
            Dai G, Zou H, Friedel RH
  TITLE     Plexin-B2 facilitates glioblastoma infiltration by modulating cell 
            biomechanics.
  JOURNAL   Commun Biol. 2021 Jan 29;4(1):145. doi: 10.1038/s42003-021-01667-4.
  PUBMED    33514835
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-01-29"; volume: "4(1):145. doi"; pages: " 
            10.1038/s42003-021-01667-4"
COMMENT     Sequence Label: pLV-PLXNB2-mGAP
FEATURES             Location/Qualifiers
     source          1..13673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..253
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    331..919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             437..448
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     846..866
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    950..974
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6519..6543)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6598..7108)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6661..6679)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    7154..7271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7538..7582)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7767..8000
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8493..8618
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8665..8845
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8846..9072)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9100..9120)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9100..9118)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9139..9155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9139..9155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9152..9174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9163..9179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9187..9193,9194..9211,9212..9217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9232..9253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9370..9387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9541..10129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9621..9640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10300..11091,11092..11160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10923..10942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11161..11265)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11291..11746)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11428..11449)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11640..11659
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11873..11895
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11887..11904
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11888..11904
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11914..11933
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11914..11932
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      11953..12088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12128..12249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12139..12158)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12193..12212
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             12321..12554
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(12645..13448)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12756..12775)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13366..13385
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(13462..13673,1..288))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13510..13529)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gcttccattg ctcagcggtg ctgtccatct gcacgagact agtgagacgt gctacttcca
       61 tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg gaacttcctg actaggggag
      121 gagtagaagg tggcgcgaag gggccaccaa agaacggagc cggttggcgc ctaccggtgg
      181 atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg
      241 ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaattaga
      301 tcactagtcc ggatatctgc agaattccag gcggggaggc ggcccaaagg gagatccgac
      361 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
      421 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
      481 tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc gacaacacca
      541 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
      601 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
      661 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
      721 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
      781 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
      841 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
      901 tgtaatccag aggttgattg tcgagcggcc gccactgtgc tggatatcaa ccactttgta
      961 caagaaagct gggcccatga ggccctagag gtcagtgacc ttgttctcca gtgcagcggc
     1021 aatctgctgc aggcggaagg ccagctgcat cttctgggcg gcaggatcct cctccaaggc
     1081 attgatgatc tcgtcatagt acttctgcgt gtattggtag agctggtgga gtgccacgag
     1141 ggtgttcaag gagtccgtgt gcgcccggga aatctctgcc aggtgtgtgt tcatgtcctg
     1201 gtcgctgacc tgcaccatct gccggatccc cttgtagtaa tcctccacca tcttcttgta
     1261 ggtggagatc tccttggcgt acagcagctt gttgctggga gaatcgcggc tcagcttatg
     1321 ctccgtgcgc gtgcaggcat ccatgaaggt ctgcgcgatg actgacagcg aggcgtccac
     1381 cacctcgtgg acatgcacgt caaagatgaa gtgggggttc ttgaggatgt tcacccagaa
     1441 ccggagcggt aagctgttcg tcttccagat gtggatggtg tcttcatcct ggatgttgtg
     1501 cttctctgcc tgctcgtcca ggaagtcgaa gaagtacttg actgcaggtg gcaccgcgtg
     1561 cccaggcgcc agcacgctct ggaagaagtt gtccacaaac tgctgcagtg tgcccttgac
     1621 tgagagcagc cgcgtcaggt agatctcggt gatggccttc gtccgctcct tctctttcac
     1681 gctgcctctc ttggacttgc cctcgtccac ctcgtcggtc ggccgcacca ggtgccacac
     1741 ccggttctcc tcctccagga gggcatggcg ctccccaggc aggtcctgct ggctgtcctc
     1801 cggctgctgg gagaccccca ccttggacag gatgagggtg gctccatccc ggacattgta
     1861 gtgcataagg gtgttgacgc gcttccaccg gccctcccgc tgtgacgtca ggtccaggtc
     1921 cgacaggatc tgcgctgtgg agcccggacg ccactccagg accacgctgt ctggcctggg
     1981 ccagcaggag cagggctgcc cacggtacac ctggtcaatg atcttctcct tgacctggga
     2041 gatggtgtca cagttgagga ccttcaccgg gatggcgtcc actccctcgt cctgcacgat
     2101 cacgctcacc gtcaggggtg cgtactccac atcatccccc agcagccccg tgtcgttgag
     2161 agtgtacttg gccttcttct gtaccgcatc caccgggccc ttttccacct gatgtttgat
     2221 ggccttgaag agcttgtaca ggggctcccc ggcactgtcc ttgaggtact ggtacaggca
     2281 gatggacatc cagttggaca gcatcctctc caccacagtc tcagagccgg ccagcatcag
     2341 cttggggttc ttggccacca cgtactgctc caggagctcc aggaagagcg tgtgcatgat
     2401 gtccgtgtag tactccagtt tcccgtgcag cgccaccgtc agcagggacg cgaagtagac
     2461 cttggcgcgg gccgagaact cccgctggtt ctccagggtg tggatgaaat tgatgaggaa
     2521 agacttgctg ttcagcaggt tggagaactg gtagagggcc tgctccacca ccggccgccg
     2581 cggctcaggg atgtccagct tgccggtgat catcacgtcc ttgtcgccgt ccttggaggg
     2641 caggaagaag acgcggtcgg tgtaggtctt gtagtccagc acggggatgc cggcctcgtg
     2701 cacgtcgttg gtctggtcct ccatctcgat catcaggtct gtgaattcct tcttgcagcg
     2761 gtcccgcacg ctctcctcca ggccctccag ctgggacttg atcttctcat actctcgttc
     2821 ggcctgctgg ctcttcctcc agtagcagta gacagacacc gcgatgacga ccaccatggg
     2881 cacgatgacc agcggcaaga tgaggctgag cggcacgtcg ctcacccgtg tgtcgtactc
     2941 cacgcggccc agcacccact cgcgagagcc gaacttcaca atgaactcgg gcaggttgtg
     3001 tgtggtgtct cgtttctgcc gccgcttggg cgggggctgc acctccgggg gctcacagta
     3061 caggtcggtc tccgtcagcg tcttcatggt gcagcgctcg gcacccacga aggcctcggc
     3121 ctcctgcagc gtcatcgcct tgttcagatt ggtgccccgg gcgtggatga gcttgttgac
     3181 ctgcttcttg acgccacctg tgaagttctc aaaggtgggg tcaggcacgt actcgaaggc
     3241 cccggcctct gttctgagca gggcacggtg cccgtccatc tcgatcagca ccgtgaggtt
     3301 gtaggcctct ggctcctcag gcacagccgg ggacaggaag acgaccttgg tgtcattgtg
     3361 gaacacgtag tctgtaccca ccaccgtcat gggctgcagg gattcagcct cccgcggcgg
     3421 ctgccaggac tgcaggggct ccgcgatgac caccatggca aacctctgga tcaggctgaa
     3481 gccctgaccc gtgacgttga tgctgcggcc accactggca aagcttcgta gcggctcgaa
     3541 ggctcgcagt acggggtttt cgcggtaggt gaagaagatg ccggggttgg gcacggggga
     3601 ccccccgtag gagacctcca gaagcatctg gccccgtgtc gcctgggggc cagtgacaca
     3661 ctggagctgc gccccaaact tcgtcacttt acacgggacg ccgttgaggg tcacccgcac
     3721 gtcctcctgg gagcccgtgt ccaggtgggt gccgtggatg gtcagtgtgg tgccgcccgc
     3781 ctgcggtccc tgctgcggct ccacactgag aggcttgggc tgttggaagg tgaactggac
     3841 attgggaggc gaacggccca gtttcccgaa gacgtccacc tcgacacccc ccgtgaaagg
     3901 cgtctccgca gcctcgatca cacacacgat ccgggtggac acggagtaac gttccggctg
     3961 aaaggagcag ttccggccgg ccacagagat cctctggatg tcccctgctt ggacgcccaa
     4021 attggacccc aggatggtga tgcggatgcc cccacccagg gggcccgtct caggctggat
     4081 cctggtgatg acgggcggcg ggcactcgga ggtggtgttg cacagggcct catacacgca
     4141 cctgctctgg cccccgcacc acgcacacct gtagtcgggg ttagcggccc ggcacaggct
     4201 gcagtcgctg cggccaaagg agcagttgta gagggtcaca tggagcttgc tgtcgatatt
     4261 cttgccgtaa gacttgacgt agaggtgcag gggcagcgtc tcgttggcat cgtgggacag
     4321 ctttggggtc cgaaaggcga aggtcccaga ttcctgcatg gtcaccggct ccatgaactt
     4381 gagcaagtca ctgcccacgt gcagggagga acccttcacg gtgtccaggt tcttgccctg
     4441 gaagttcaca tctgtctcgt ggttcatggg gatcaccagg gggctgggtc ccaggaactg
     4501 gggacagctg tcctccatgt gggcacggac gatgccgtcc tcagggttgg gcgaagcctc
     4561 ccggcactcg tggtagcgca ggtcccactg gcaggtccag cggttgctca cgcaggagat
     4621 gcacggcagg ttctcctcca ggctcatggc ctggcggcag tcgtagaagg ggtactggta
     4681 ggacgtgagg aagatgttgc ctcgtctaag gaggagctgg atggtcacgg ccacgtggtc
     4741 ctggcctggc ggtgtgacgg ggatgctgct tggggagttg cagatgacgg cctcgccctc
     4801 cacgcgggcg gggtgtggcg gcgactcccc aaaaaggcac agcaactcgt cctcctcgct
     4861 cagggcaggg agggggctga cggtcagctg cacctccccc tgggcccgcc ggctcatgtt
     4921 ctgtggctgg gcgctggtga cggccacgca ggacttgctt cggctccaca gccagtggct
     4981 ggcctcctcg gcccgcggac actcggcctt ccgggtgcat cgtccctcga cgacgcacca
     5041 gccgcagtag gggtcctggg agtcgcggca ctgggtgcag gtcgggtagc tcaggcactc
     5101 ctgcaccggc agccggaaca ccttgtcctg ggtcatggcg tacaggctgc ccaggtctcc
     5161 agacagtacc aggtcgcgct tgactctctt gtttatctcc acaaggatag agtcgtactc
     5221 tgaggaggtg ccatctgggg tgaggtacac cttgaggatc cggccatcag aggtgcccag
     5281 aaaagcaaca gtgtggttat tttcagcagc cacggtgaca gccgtgaggt tcaggcctcc
     5341 acgctgcagc acggctgtgc ctctgagccc gtcgcggctg cccagcgggt agggcaggtg
     5401 ctccgagcca catgggaagc tcttgctgga gcccggcgcg tggccgccgc actggatatc
     5461 gccgtggaag ggcttgtaga agatgtcacg ggcctcccgg gtgcctgtgt aacaggcgtt
     5521 gcggttggcc tccatcttgg cgtgcacctt gtccagcggg aacaggcaga ggcccgcacc
     5581 gggcccccca ctgctccggc tgtctctgct gaagacagca tatagcaccc tgccagagcc
     5641 aggcgcagcc acggaggcgg ccaggcaggt gccaaaggca gcggcgtgga tgtcggggtc
     5701 ccggcactgc aggtccatct ccaggtagga gtagtagttg gggtcttctc tgcacatgcg
     5761 tgccagcagc gtgcggttcc gggccgggtg cttgtcctgc tggttgaaga caaagaagac
     5821 gtaggggccg tcctcgaagg ccgccacgaa ctgctgtgtg ttggtggaca ggtagccggc
     5881 cttgtaggtg gcgtggtccg tgtaggcttc aaaggcctcc ctgctgtcag tccggtccaa
     5941 cagccgagtg ctcacgatga tgccgttgtc gtgtggccca ttgcctttgc ccacaaacag
     6001 cacgcggtca ccaccaggac ccgtggagct caccagcccc actgtggcca cgccctcatc
     6061 attgctggcc acgaaagact tctccccgct gccgtcctcg tagaacaggc ggagggagat
     6121 gttgctcagg gcgcgcagag cgcagatgcc cttgaagagg ctgccgcact ccaccaggcg
     6181 cttcctggga gggtcgagca gcagcagctg gttgacattg tcagtcatct cagcctcatg
     6241 gcactggctg gcctcgatgg gcggcgtgca cttcttgttg tccagggccg ggcccgtggc
     6301 cacctgctgc tccagctgca gcttcgcatc cagctggtag agggcattca ccgcccccag
     6361 gtacaccacg cctgaggcct catccacagc caggtggttc agctctttct cgctgcggaa
     6421 gaagtccagc ttgcggggcc tcaggctggc acctgcgccc agcaggccca gcagggtcag
     6481 ggcccagagc tgcagtgcca tggtgggcct tgacggccct tctgcttttt tgtacaaact
     6541 tgttgatatc tgcagaattc caccacactg gactagtcga cgaaccggtg gatccccctg
     6601 gggagagagg tcggtgattc ggtcaacgag ggagccgact gccgacgtgc gctccggagg
     6661 cttgcagaat gcggaacacc gcgcgggcag gaacagggcc cacactaccg ccccacaccc
     6721 cgcctcccgc accgcccctt cccggccgct gctctcggcg cgccctgctg agcagccgct
     6781 attggccaca gcccatcgcg gtcggcgcgc tgccattgct ccctggcgct gtccgtctgc
     6841 gagggtacta gtgagacgtg cggcttccgt ttgtcacgtc cggcacgccg cgaaccgcaa
     6901 ggaaccttcc cgacttaggg gcggagcagg aagcgtcgcc ggggggccca caagggtagc
     6961 ggcgaagatc cgggtgacgc tgcgaacgga cgtgaagaat gtgcgagacc cagggtcggc
     7021 gccgctgcgt ttcccggaac cacgcccaga gcagccgcgt ccctgcgcaa acccagggct
     7081 gccttggaaa aggcgcaacc ccaaccccgt ggaattcgat atcaagcttc tcgaggctag
     7141 tctcgtgatc gataaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta
     7201 tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca atcccccctt
     7261 ttcttttaaa agttaaccga taccgtcgag atccgttcac taatcgaatg gatctgtctc
     7321 tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg
     7381 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
     7441 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
     7501 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
     7561 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta
     7621 ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa
     7681 tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga
     7741 gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag
     7801 ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt
     7861 gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc
     7921 tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga
     7981 acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct
     8041 ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat ttatataatt
     8101 cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc ggccgcttgc
     8161 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     8221 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     8281 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     8341 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     8401 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     8461 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     8521 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     8581 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     8641 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     8701 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     8761 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     8821 agatctggtc taaccagaga gacccgttta tgtatcgagc taggcactta aatacaatat
     8881 ctctgcaatg cggcaattca gtggttcgtc caatccatgt cagacccgtc tgttgccttc
     8941 ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
     9001 ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagagtattg
     9061 cataagacta cattaagctt gcagctccag cttttgttcc ctttagtgag ggttaattgc
     9121 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9181 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9241 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9301 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9361 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9421 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9481 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9541 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9601 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9661 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9721 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9781 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9841 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9901 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9961 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10021 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10081 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10141 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10201 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10261 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10321 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10381 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10441 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10501 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10561 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10621 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10681 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10741 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10801 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10861 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10921 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10981 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11041 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11101 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11161 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11221 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11281 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
    11341 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
    11401 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
    11461 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
    11521 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
    11581 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
    11641 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
    11701 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
    11761 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11821 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    11881 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
    11941 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
    12001 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
    12061 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
    12121 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
    12181 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
    12241 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
    12301 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
    12361 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
    12421 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
    12481 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
    12541 agctagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
    12601 gtaagtcatt ggtcttaaag gtaccgggcc ctcgacggat cccctcagaa gaactcgtca
    12661 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
    12721 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
    12781 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
    12841 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
    12901 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
    12961 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
    13021 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
    13081 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
    13141 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
    13201 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
    13261 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
    13321 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
    13381 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatggcc
    13441 gatcccatat tggctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc
    13501 agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc
    13561 cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc
    13621 ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta ccc
//
