LOCUS       sequence-171486-       11399 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Alexandrov A, Shu MD, Steitz JA
  TITLE     Fluorescence Amplification Method for Forward Genetic Discovery of 
            Factors in Human mRNA Degradation.
  JOURNAL   Mol Cell. 2017 Jan 5;65(1):191-201. doi: 
            10.1016/j.molcel.2016.11.032. Epub 2016 Dec 22.
  PUBMED    28017590
REFERENCE   2  (bases 1 to 11399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.032"; journalName: "Mol Cell"; date: 
            "2017-01-5- 5"; volume: "65"; issue: "1"; pages: "191-201"
COMMENT     Sequence Label: Launchpad (AVA2590)
FEATURES             Location/Qualifiers
     source          1..11399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1268..1288
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1331..1511
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2893..3126
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3388..3505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(3563..3583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3581..3878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3748..3882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    3892..3939
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    4053..4134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4090..4109)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4144..4163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(4173..4886,4887..4889,4890..4892))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4176..4886,4887..4889,4890..4892))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4218..4239)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4567..4586
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4826..4847
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(4928..6110)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4937..5879
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4960..4980)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        6412..6911
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6447..6470)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6844..6863
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6932..7531
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6932..6951)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7428..7448
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    7538..7585
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    7874..8098
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8231..8250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(9272..9289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9443..10031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9523..9542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10202..10993,10994..11062))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10825..10844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11063..11167)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11235..11253
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11291..11313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
       61 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
      121 cgggcctctt cgctattacg ccaggggagg cagagattgc agtaagctga gatcgcagca
      181 ctgcactcca gcctgggcga cagagtaaga ctctgtctca aaaataaaat aaataaatca
      241 atcagatatt ccaatctttt cctttattta tttatttatt ttctattttg gaaacacagt
      301 ccttccttat tccagaatta cacatatatt ctatttttct ttatatgctc cagttttttt
      361 tagaccttca cctgaaatgt gtgtatacaa aatctaggcc agtccagcag agcctaaagg
      421 taaaaaataa aataataaaa aataaataaa atctagctca ctccttcaca tcaaaatgga
      481 gatacagctg ttagcattaa ataccaaata acccatcttg tcctcaataa ttttaagcgc
      541 ctctctccac cacatctaac tcctgtcaaa ggcatgtgcc ccttccgggc gctctgctgt
      601 gctgccaacc aactggcatg tggactctgc agggtcccta actgccaagc cccacagtgt
      661 gccctgaggc tgccccttcc ttctagcggc tgcccccact cggctttgct ttccctagtt
      721 tcagttactt gcgttcagcc aaggtctgaa actaggtgcg cacagagcgg taagactgcg
      781 agagaaagag accagcttta cagggggttt atcacagtgc accctgacag tcgtcagcct
      841 cacagggggt ttatcacatt gcaccctgac agtcgtcagc ctcacagggg gtttatcaca
      901 gtgcaccctt acaatcattc catttgattc acaatttttt tagtctctac tgtgcctaac
      961 ttgtaagtta aatttgatca gaggtgtgtt cccagagggg aaaacagtat atacagggtt
     1021 cagtactatc gcatttcagg cccgagagct gcatccggag tatctagatg gagttccgcg
     1081 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1141 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     1201 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     1261 ccgttgacgc aaatgggcgg taggcgtgta ctctagaagg tctatataag cagagctcgt
     1321 ttagtgaacc gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     1381 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
     1441 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1501 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc agaggagatc
     1561 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     1621 cagagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1681 gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa ggccaggggg
     1741 aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1801 agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg gacagctaca
     1861 accatccctt cagacaggat cagaagaact tagatcatta tataatacaa tagcagtcct
     1921 ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag ataagataga
     1981 ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca caggaaacaa
     2041 cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa tggtacatca
     2101 ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag
     2161 cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa
     2221 taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat
     2281 caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc ctattgcacc
     2341 aggccagatg agagaaccaa ggggaagtga catagcagga actactagca aattaagaga
     2401 acaatttgga aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat
     2461 tgtaacgcac agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt
     2521 taatagtact tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag
     2581 tgacacaatc acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg
     2641 aaaagcaatg tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg
     2701 gctgctatta acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg
     2761 aggaggcgat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     2821 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     2881 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     2941 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca
     3001 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3061 gggcatcaaa cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3121 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3181 tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg
     3241 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3301 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggctt gcggccggaa
     3361 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     3421 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     3481 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     3541 ggcggccgcg cgaggcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3721 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3841 tctgagctat tccagaagta gtgaggaggc ttttttggag gctaccatgg agaagttcct
     3901 attccgaagt tcctattctc tagaaagtat aggaacttca agcttggtcg accctcttaa
     3961 gtaccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     4021 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     4081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4141 gttgtggttt gtccaaactc atcgagctcg acttacttgt acagctcgtc catgccgaga
     4201 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
     4261 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
     4321 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
     4381 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
     4441 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
     4501 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
     4561 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
     4621 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
     4681 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
     4741 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
     4801 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
     4861 ccggtgaaca gctcctcgcc cttgctcacc atggtggact agttctagag cggaaacgct
     4921 agagttttca cgacacctga aatggaagaa aaaaactttg aaccactgtc tgaggcttga
     4981 gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac atcaagtgcc
     5041 aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc gcataaaacc
     5101 cctcccccca acctaaagac gacgtactcc aaaagctcga gaactaatcg aggtgcctgg
     5161 acggcgcccg gtactccgtg gagtcacatg aagcgacggc tgaggacgga aaggcccttt
     5221 tcctttgtgt gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc
     5281 tccctgcagc agggccggga agcggccatc tttccgctca cgcaactggt gccgaccggg
     5341 ccagccttgc cgcccagggc ggggcgatac acggcggcgc gaggccaggc accagagcag
     5401 gccggccagc ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc
     5461 tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg
     5521 aaataccagt gtgcagatct tggcccgcat ttacaagact atcttgccag aaaaaaagcg
     5581 tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca gcgagacagg
     5641 cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg cccaggccag gcctcaactc
     5701 aagcacgagg cgaaggggct ccttaagcgc aaggcctcga actctcccac ccacttccaa
     5761 cccgaagctc gggatcaaga atcacgtact gcagccaggg gcgtggaagt aattcaaggc
     5821 acgcaagggc cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc
     5881 tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata
     5941 tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag
     6001 tttaccccgc gccactttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc
     6061 tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc tcacgcatgc
     6121 tcttctccac ctcagtgatg acgagagcgg gcgggtgagg gggcgggaac gcagcgatct
     6181 ctgggttcta cgttagtggg agtttaacga cggtccctgg gattccccaa ggcaggggcg
     6241 agtccttttg tatgaattac tctcagctcc ggtcggggcg ggttgggggg ggtggtgacg
     6301 gggaggccgc ctggaaggga cgtgcagaat cttccctcta ccattgctgg cttagctcca
     6361 aagcttatcg atgataagct gtcaaacatg agaattgcta gcaattctac cgggtagggg
     6421 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     6481 gctacacaag tggcctctgg cctcgcacac attccacatc catcggtagg cgccaaccgg
     6541 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     6601 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     6661 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     6721 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     6781 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     6841 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     6901 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     6961 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     7021 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     7081 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7141 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7201 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     7261 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     7321 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     7381 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     7441 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7501 ctggtgcatg acccgcaagc ccggtgcctg aggatccgaa gttcctattc cgaagttcct
     7561 attctctaga aagtatagga acttcagatc cgcggactag tgaattccag ctgagcgccg
     7621 gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggggggatc
     7681 tcgactctag aagcttggta cctttaagac caatgactta caaggcagct gtagatctta
     7741 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccag ggtctctctg
     7801 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7861 tcataaagct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7921 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7981 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8041 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8101 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     8161 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8221 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctgtgcg
     8281 ggagaacgga gttctattat gactcaaatc agtctcccca agcattcggg gatcagagtt
     8341 tttaaggata acttagtgtg tagggggcca gtgagttgga gatgaaagcg tagggagtcg
     8401 aaggtgtcct tttgcgccga gtcagttcct gggtgggggc cacaagatcg gatgagccag
     8461 tttatcaatc cgggggtgcc agctgatcca tggagtgcag ggtctgcaaa atatctcaag
     8521 cactgattga tcttaggttt tacaatagtg atgttacccc aggaacaatt tggggaaggt
     8581 cagaatcttg tagcctgtag ctgcatgact cctaaaccat aatttctttt ttgttttttt
     8641 ttttttattt ttgagacagg gtctcactct gtcacctagg ctggagtgca gtggtgcaat
     8701 cacagctcac tgcagcctca acgtcgtaag ctcaagcgat cctcccacct cagcctgcct
     8761 ggtagctgag actacaagcg acgccccagt taatttttgt atttttggta gaggcagcgt
     8821 tttgccgtgt ggccctggct ggtctcgaac tcctgggctc aagtgatcca gcctcagcct
     8881 cccaaagtgc tgggacaacc ggggccagtc actgcacctg gccctaaacc ataatttcta
     8941 atcttttggc taatttgtta gtcctacaaa ggcagtctag tccccaggca aaaagggggt
     9001 ttgtttcggg aaagggctgt tactgtcttt gtttcaaact ataaactaag ttcctcctaa
     9061 acttagttcg gcctacaccc aggaatgaac aaggagagct tggaggttag aagcacgatg
     9121 gaattggtta ggtcagatct ctttcactgt ctgagttata attttgcaat ggtggttcaa
     9181 agactgcccg cttctgacac cagtcgctgc attaatgaat cggccaacgc gcggggagag
     9241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9841 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    10021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    10141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    10201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    11041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    11101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    11161 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    11221 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
    11281 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    11341 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggc
//
