LOCUS       sequence-172524-        1144 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector expressing shRNA against murine DNMT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Leoni C, Montagner S, Rinaldi A, Bertoni F, Polletti S, Balestrieri 
            C, Monticelli S
  TITLE     Dnmt3a restrains mast cell inflammatory responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 
            10.1073/pnas.1616420114.
  PUBMED    28167789
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 
            10.1073/pnas.1616420114."
COMMENT     Sequence Label: pAPM sh2 DNMT1
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             181..780
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(181..200)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     677..697
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           830..951
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 tttccaggct cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag
       61 cctgcttctc gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa
      121 cccctcactc ggcgcgccag tcctccgaca gactgagtcg cccgggggtc tagagccacc
      181 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
      241 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
      301 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
      361 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
      421 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
      481 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
      541 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
      601 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
      661 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
      721 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
      781 gcggccgcaa gccttgttaa gtgctcgttc ggcagcacat atactatgtt tgaatgaggc
      841 ttcagtactt tacagaatcg ttgcctgcac atcttggaaa cacttgctgg gattacttct
      901 tcaggttaac ccaacagaag gctcgagaag gtatattgct gttgacagtg agcgcacaaa
      961 cgctctcatc gacaagtgtg acgtcgaaga ctcctgactt gtcgatgaga gcgtttgtat
     1021 gcctactgcc tcggaattca aggggctact ttaggagcaa ttatcttgtt tactaaaact
     1081 gaatacctgc tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa
     1141 atca
//
