LOCUS       sequence-172987-        8145 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For  expression of PLEKHM1 tagged with HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M
  TITLE     Atg8 family LC3/GABARAP proteins are crucial for 
            autophagosome-lysosome fusion but not autophagosome formation during
            PINK1/Parkin mitophagy and starvation.
  JOURNAL   J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18.
  PUBMED    27864321
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-12-19"; volume: "215(6)"; pages: "857-874. Epub 
            2016 Nov 18"
COMMENT     Sequence Label: pBMN PLEKHM1-HA
FEATURES             Location/Qualifiers
     source          1..8145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             541..1010
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1075..1432
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1497..1913
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1843..1865
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1881..1897
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1923..2297
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2235..2251
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             5474..5500
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             5552..6145
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6276..6293)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6447..7035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6527..6546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7209..8000,8001..8069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7832..7851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     join(8137..8145,1..10)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcacgaggc cctttcgtct tcaagaacag ctttgctctt aggagtttcc taatacatcc
       61 caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta
      121 agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat
      181 ttcataaggg tttcaatgtg catgaatgct gcaatattcc tgttaccaaa gctagtataa
      241 ataaaaatag ataaacgtgg aaattactta gagtttctgt cattaacgtt tccttcctca
      301 gttgacaaca taaatgcgct gctgagaagc cagtttgcat ctgtcaggat caatttccca
      361 ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga catatacatg
      421 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
      481 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
      541 ctgaatatgg gccaaagcgg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      601 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      661 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      721 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      781 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      841 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      901 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      961 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     1021 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     1081 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     1141 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     1201 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
     1261 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
     1321 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     1381 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     1441 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
     1501 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1561 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1621 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1681 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1741 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1801 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1861 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1921 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1981 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     2041 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     2101 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     2161 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     2221 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     2281 gaccatcctc tagactgccg gatccatgct ttcagtggtg gagaatggac tggaccccca
     2341 ggctgccatc ccggtcatca agaagaagct ggtgggatcc gtgaaggcct tgcagaagca
     2401 gtacgtgtcc ctggacacgg tggtcactag tgaagacgga gatgccaaca ccatgtgcag
     2461 cgccctggag gccgtattta tccatggcct gcacgccaag cacatccgag ctgaggccgg
     2521 aggaaaaagg aagaaaagtg cccaccagaa gcctctgccc cagcctgtct tctggcccct
     2581 cctgaaagct gtcacccaca aacacatcat ctcagagttg gagcacctga cgtttgtcaa
     2641 cacggatgtg ggccgctgcc gggcatggct gcggctggcc ctgaacgatg gcctgatgga
     2701 gtgctacctg aagctgctgc tgcaggagca ggcccgcttg catgagtact accagcccac
     2761 cgccctgctc cgggatgctg aggagggcga gttcctcctt agcttcctgc agggcctcac
     2821 gtccttgtcc ttcgaactct cctacaagtc tgccatctta aatgagtgga cgctcacccc
     2881 attggccctg tctgggcttt gcccgctttc tgagctggac cctctctcta cctctggtgc
     2941 agaactacag cggaaggaat ctctggattc catttcccat tcttcaggct ctgaagacat
     3001 cgaagtccat cactcgggcc ataagatacg gaggaaccag aagctgactg cctcctccct
     3061 cagcctggac acggccagtt catcccagct gtcctgcagc ctaaactctg atagctgctt
     3121 actccaagag aatggctcca agagtccaga ccattgcgag gagcccatgt cctgtgactc
     3181 agacctgggc acagcaaatg ctgaggactc agaccggtct ctgcaagagg tattgttgga
     3241 attcagcaaa gcccaggtaa actctgtgcc aaccaacgga ctgagccaag aaacagagat
     3301 ccccacacca caggcctcgc tctccctcca tggcctcaac accagcacat acctgcactg
     3361 tgaggcacct gcagagcccc ttcctgccca ggcagcctct ggaactcaag atggtgtcca
     3421 cgtgcaggag ccgcgtcccc aggcgcccag ccccctggac ttacagcagc ctgtagagag
     3481 cacctcaggc cagcagcctt ctagtactgt cagcgagaca gccagagaag tgggccaagg
     3541 gaatggcctg cagaaggccc aggctcatga cggagctggt ctgaagctgg tagtttcctc
     3601 acccaccagt ccgaaaaaca agagctggat ctcagaggat gacttctacc ggccttcccg
     3661 ggagcaaccc ctggagagtg cttcagacca cccaatagct tcttacaggg ggactccagg
     3721 gtcaaggcct ggtctccaca ggcatttttc tcaagaacca agaaaaaact gctccctggg
     3781 ggcgttagac caagcgtgtg taccttcccc aggaagaagg caagcccagg cagccccatc
     3841 ccaggggcat aagagcttcc gggtggtaca ccggagacag atgggactgt ccaacccatt
     3901 ccggggtctc atgaagctgg gcaccgtgga gcggcggggg gcaatgggca tctggaagga
     3961 gctcttctgc gagctctccc cgctggagtt ccgcctctac ctgagcaacg aggagcacac
     4021 ctgtgtggag aactgctcgc tgcttcgctg tgagtctgtg gggccagccc atagtgatgg
     4081 gcgctttgag ctggtcttct ctggcaagaa gctggccctg cgcgcctcct cccaggacga
     4141 agctgaggac tggctggacc gggtgcggga ggccctgcag aaggtccggc ctcagcagga
     4201 ggatgagtgg gtgaacgtgc agtacccaga ccagcctgag gaaccccccg aggcgcccca
     4261 gggctgcctc tctccctcag acctgctctc ggagcccgcg gccctccagg gcacacagtt
     4321 tgactggtcg tccgcccagg ttccagagcc agatgccatc aaggagtccc tgctgtactt
     4381 gtacatggac aggacctgga tgccctatat attttctctg tccttggagg ctctgaaatg
     4441 tttccgcatc aggaacaatg agaagatgct gagtgacagc cacggcgtgg agaccatccg
     4501 ggacatcctg ccagacacca gccttggggg cccatccttc ttcaaaatca tcacggccaa
     4561 ggctgtcctg aagctgcagg ccggaaacgc cgaggaagcc gccctgtgga gggatctggt
     4621 ccgcaaagtc ctggcatcct acttggagac agccgaggag gcggtgaccc tgggcgggag
     4681 cctggatgaa aactgtcagg aggtgctgaa atttgccacc cgggagaatg gcttcctgct
     4741 gcagtacctg gtggctatcc ccatggagaa aggccttgac tcccaaggct gcttctgcgc
     4801 aggctgctcc cggcagatcg gcttctcctt tgtacgaccc aagctctgtg ccttctctgg
     4861 cctctattac tgtgacatct gccaccaaga cgatgcctca gtgattccgg ccaggatcat
     4921 ccacaactgg gacctcacca agcgcccgat ctgcaggcag gccctgaagt ttctgacaca
     4981 gatccgggcc cagcccctca tcaacctgca gatggtgaac gcgtctctgt acgagcatgt
     5041 ggagcggatg cacctcattg ggaggagacg ggagcagctg aagctcctgg gggattacct
     5101 gggcctgtgc cggagtggcg ccctgaagga gctcagcaag aggctcaacc acaggaatta
     5161 tctcttggaa tctccgcata ggttcagtgt tgctgacctc caacagatcg cagacggggt
     5221 gtatgaagga ttcctcaagg ccctgattga atttgcctcc cagcatgtct accactgcga
     5281 cctgtgcacc cagcgcggct tcatctgcca gatctgccag caccacgaca tcatcttccc
     5341 ctttgagttt gacaccacag tcaggtgtgc cgagtgcaag accgtcttcc accagagctg
     5401 ccaggctgtg gtgaagaagg gctgcccccg ctgtgcccgc cggcgcaagt accaggaaca
     5461 gaacattttc gcctacccat acgatgttcc agattacgct taagtcgacg ataaaataaa
     5521 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     5581 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     5641 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     5701 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     5761 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     5821 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     5881 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     5941 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     6001 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     6061 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     6121 tacccgtcag cgggggtctt tcattcacat gcagcatgta tcaaaattaa tttggttttt
     6181 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
     6241 gagaggcggt ttgcgtattg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6301 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     6901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7141 cttttaaatt aaaaatgaag tttgcgcaaa tcaatctaaa gtatatatga gtaaacttgg
     7201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7801 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     7861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8101 tatcagggtt attgtctcat gacattaacc tataaaaata ggcgt
//
