LOCUS       sequence-173289-       11746 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral expression construct encoding SR-B1 and GFP from separate
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11746)
  TITLE     Grove lab plasmids
REFERENCE   2  (bases 1 to 11746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDUAL SRBI (GFP)
FEATURES             Location/Qualifiers
     source          1..11746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2767..2776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4398..4418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4816..4827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5406..5424
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5502..5511
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5508..5510,5511..5513,5514..6227)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5508..5510,5511..5513,5514..6227)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5553..5574)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5814..5833)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6161..6182
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6323..6556
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6581..6599)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6582..6599)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6889..6908)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7122..7141)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7241..7263
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7301..7319)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7387..7491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7492..7560,7561..8352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7710..7729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8523..9111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9012..9031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9265..9282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9352..9372)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9357..9686
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9537..9672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10820..10885
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11015..11035
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11460..11594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11497..11516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11551..11570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg atccactagt gcggccgcga ctctagagtc
     2761 gacgccgcca ccatgggctg ctccgccaaa gcgcgctggg ctgccggggc gctgggcgtc
     2821 gcggggctac tgtgcgctgt gctgggcgct gtcatgatcg tgatggtgcc gtcgctcatc
     2881 aagcagcagg tccttaagaa cgtgcgcatc gaccccagta gcctgtcctt caacatgtgg
     2941 aaggagatcc ctatcccctt ctatctctcc gtctacttct ttgacgtcat gaaccccagc
     3001 gagatcctga agggcgagaa gccgcaggtg cgggagcgcg ggccctacgt gtacagggag
     3061 ttcaggcaca aaagcaacat caccttcaac aacaacgaca ccgtgtcctt cctcgagtac
     3121 cgcaccttcc agttccagcc ctccaagtcc cacggctcgg agagcgacta catcgtcatg
     3181 cccaacatcc tggtcttggg tgcggcggtg atgatggaga ataagcccat gaccctgaag
     3241 ctcatcatga ccttggcatt caccaccctc ggtgaacgtg ccttcatgaa ccgcactgtg
     3301 ggtgagatca tgtggggcta caaggacccc cttgtgaatc tcatcaacaa gtactttcca
     3361 ggcatgttcc ccttcaagga caagttcgga ttatttgctg agctcaacaa ctccgactct
     3421 gggctcttca cggtgttcac gggggtccag aacatcagca ggatccacct cgtggacaag
     3481 tggaacgggc tgagcaaggt tgacttctgg cattccgatc agtgcaacat gatcaatgga
     3541 acttctgggc aaatgtggcc gcccttcatg actcctgagt cctcgctgga gttctacagc
     3601 ccggaggcct gccgatccat gaagctaatg tacaaggagt caggggtgtt tgaaggcatc
     3661 cccacctatc gcttcgtggc tcccaaaacc ctgtttgcca acgggtccat ctacccaccc
     3721 aacgaaggct tctgcccgtg cctggagtct ggaattcaga acgtcagcac ctgcaggttc
     3781 agtgccccct tgtttctctc ccatcctcac ttcctcaacg ccgacccggt tctggcagaa
     3841 gcggtgactg gcctgcaccc taaccaggag gcacactcct tgttcctgga catccacccg
     3901 gtcacgggaa tccccatgaa ctgctctgtg aaactgcagc tgagcctcta catgaaatct
     3961 gtcgcaggca ttggacaaac tgggaagatt gagcctgtgg tcctgccgct gctctggttt
     4021 gcagagagcg gggccatgga gggggagact cttcacacat tctacactca gctggtgttg
     4081 atgcccaagg tgatgcacta tgcccagtac gtcctcctgg cgctgggctg cgtcctgctg
     4141 ctggtccctg tcatctgcca aatccggagc caagagaaat gctatttatt ttggagtagt
     4201 agtaaaaagg gctcaaagga taaggaggcc attcaggcct attctgaatc cctgatgaca
     4261 tcagctccca agggctctgt gctgcaggaa gcaaaactgt aggtcgacct gcaggcatgc
     4321 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4381 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4441 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4501 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4561 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4621 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4681 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4741 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4801 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg tcgaattccc
     4861 acggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     4921 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     4981 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     5041 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     5101 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcarkggca
     5161 gcscgaccgc gatccgaagg ttccttgcgg tkcgcggcgt gccggacgtg acaaacggaa
     5221 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     5281 cgaccgcgat gggctgtggc caatagtggc tgctcagcag ggcgcgccga gagcagcggc
     5341 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     5401 gcacggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     5461 gaccgaatca ccgacctctc tccccaggac gcgtaccggt cgccaccatg gtgagcaagg
     5521 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     5581 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     5641 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     5701 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     5761 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     5821 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5881 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5941 actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga
     6001 acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     6061 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     6121 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     6181 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaact cgagggaatt
     6241 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6301 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6361 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6421 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6481 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6541 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     6601 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     6661 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     6721 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     6781 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
     6841 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     6901 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     6961 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     7021 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     7081 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
     7141 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7201 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7261 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7321 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7381 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7441 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7501 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7561 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7621 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7681 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7741 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7801 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7861 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7921 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7981 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8041 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8101 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8161 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8221 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8281 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8341 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8401 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8461 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8641 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     8701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8881 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9301 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
     9361 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9421 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     9481 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     9541 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9601 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     9661 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
     9721 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
     9781 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
     9841 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
     9901 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
     9961 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
    10021 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
    10081 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
    10141 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
    10201 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
    10261 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
    10321 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
    10381 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
    10441 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
    10501 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
    10561 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
    10621 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
    10681 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
    10741 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
    10801 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
    10861 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
    10921 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
    10981 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
    11041 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
    11101 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
    11161 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
    11221 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
    11281 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
    11341 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
    11401 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    11461 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    11521 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    11581 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    11641 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    11701 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtac
//
