LOCUS       sequence-173291-        8930 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses lncRNA MONC (Alias: MIR99AHG; LINC00478).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Emmrich S, Streltsov A, Schmidt F, Thangapandi VR, Reinhardt D, 
            Klusmann JH
  TITLE     LincRNAs MONC and MIR100HG act as oncogenes in acute 
            megakaryoblastic leukemia.
  JOURNAL   Mol Cancer. 2014 Jul 15;13:171. doi: 10.1186/1476-4598-13-171.
  PUBMED    25027842
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Cancer."; date: "2014-07-15"; volume: "13:171. doi"; pages: " 
            10.1186/1476-4598-13-171"
COMMENT     Sequence Label: LeGO-lnc-MONC
FEATURES             Location/Qualifiers
     source          1..8930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..53)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        225..604
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        606..804
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     759..779
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             822..1002
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1049..1174
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1671..1904
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2089..2133
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2431..2548
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2607..2623
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2665..2698)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3821..4045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4052..4550
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4086..4109)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4483..4502
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4568..4577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4574..4576,4577..4579,4580..5290)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4619..4640)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4880..4899)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5227..5248
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5291..5686
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(5717..5750)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5806..6394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5859..5879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6277..6288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6397..6413)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6923..7103
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7165..7753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7245..7264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7924..8715,8716..8784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8547..8566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8785..8889)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
       61 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
      121 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
      181 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      241 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      301 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      361 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      421 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      481 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      541 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      601 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      661 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      721 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      781 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
      841 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      901 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      961 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     1021 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     1081 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     1141 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     1201 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1261 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1321 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1381 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1441 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1501 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     1561 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1621 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1681 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1741 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1801 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1861 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1921 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1981 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2041 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2101 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2161 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2221 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2281 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2341 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     2401 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2461 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2521 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     2581 ttggttagta ccgggcccgc tctagtcgag gtcgacggta tcgataagct cgcttcacga
     2641 gattccagca ggtcgaggga cctaataact tcgtatagca tacattatac gaagttatat
     2701 taagggttcc aagcttaagc ggccgctgaa agaccccacc tgtaggtttg gcaagctagc
     2761 tgcagtaacg ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag
     2821 atcaagggcg ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc
     2881 agtttcggcc ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg
     2941 aggccaagaa cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat
     3001 cagatgtttc caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc
     3061 aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc
     3121 tcacaacccc tcactcggcg cgccagtcct ccgacctagg agagaagccg gcactcagat
     3181 tgacaggcaa ataagccagc ttgtggaagg ggcctctggg tggcagctct gggcttttgg
     3241 gtgttggaca accataggca aaactgaaat ggaacagggg cagaaaatag cacactttta
     3301 ctctacgcaa ctgttgcttt ttcatgatct tcaggaattt tactgggata attatccaaa
     3361 taaattgcaa gcattctatc caaatggagc tctttctgag atgaagagaa ttctcaatgt
     3421 caagatttga acaagaagag aatggaatac acaatatgga catccataaa aattcatcag
     3481 agagcatttt actactgagc tgcaaaggga aaaacttaaa atggatatga aaagtgaaga
     3541 aagtgatcat aggagaaaac catttcagat gacaagagca cctcaaaggc agcagcctca
     3601 aggagcagcc atggccccag acttgtcgca cggatgcaga aaacttaatg gaggaggctg
     3661 aggtcagaat gggaagagtt tttaaaaaat aaaaagggga gctaatatgt gaggaactga
     3721 ttgctgtgca ttattttcca ctgaacagac cttcttttaa cttctaatct caagcacata
     3781 gcagctgttg ttttcatctc gagttataat taattaacga ctgtgccttc tagttgccag
     3841 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3901 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3961 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4021 gctggggatg cggtgggctc tatggatgca tggtagggga ggcgcttttc ccaaggcagt
     4081 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     4141 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     4201 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     4261 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     4321 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     4381 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     4441 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     4501 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     4561 ggatcccgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4621 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4681 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4741 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc
     4801 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4861 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4921 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4981 catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga
     5041 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
     5101 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5161 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     5221 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5281 gctgtacaag gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     5341 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     5401 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     5461 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     5521 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     5581 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     5641 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaatgta caagtaagaa
     5701 ttcgtcgagg gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa
     5761 gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca acctctggat
     5821 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5881 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5941 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6001 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6061 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6121 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6181 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6241 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6301 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6361 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga
     6421 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     6481 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     6541 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6601 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     6661 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     6721 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     6781 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6841 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     6901 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6961 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7021 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7081 gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7141 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7201 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7261 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7321 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7381 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7441 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7501 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7561 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7621 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7681 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7741 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7801 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7861 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7921 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7981 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8041 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8101 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8161 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8221 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8281 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8341 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8401 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8461 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8521 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8581 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8641 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8701 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8761 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8821 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8881 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
//
