LOCUS       sequence-173373-        2403 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Type 3b, Red fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Obst U, Lu TK, Sieber V
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion 
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
  PUBMED    28252957
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337."
COMMENT     Sequence Label: pPTK018-3b-RFP_Sec
FEATURES             Location/Qualifiers
     source          1..2403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(755..789)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(860..1519)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1434..1453
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1701..2289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2190..2209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaacttccg gtatggtgtc aaagggagag gaaaataata tggctattat taaggagttt
       61 atgcgtttta aggtacatat ggaaggttct gtcaacggtc acgaattcga aattgaaggt
      121 gagggggagg ggaggccata cgagggaact cagactgcta agttaaaggt cactaaaggt
      181 ggtcctttac ctttcgcctg ggatatcctg tctccacagt ttatgtacgg ttcaaaggct
      241 tatgtgaaac atcctgccga tatcccagat tatcttaaac tttctttccc tgagggtttt
      301 aagtgggaga gggtaatgaa ctttgaagac ggtggtgtgg tcactgttac tcaggactca
      361 agtctgcagg acggtgagtt catctacaag gtgaagctga gaggtaccaa ttttccatca
      421 gatggtcccg tgatgcaaaa aaagacaatg ggttgggaag cttctagtga acgtatgtat
      481 cccgaagatg gagctttgaa aggtgaaatt aagcaaagac taaaacttaa ggatggtgga
      541 cattacgatg ctgaagttaa gacgacctac aaggccaaaa agccagtcca gttgcctgga
      601 gcatacaatg ttaacatcaa attggatata acttcccata atgaagacta taccatcgtc
      661 gagcaatacg aacgagccga agggagacac agtactggtg gtatggatga actttataaa
      721 ggatccggaa ccgcaggatc ctgagaccag accaataaaa aacgcccggc ggcaaccgag
      781 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      841 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
      901 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
      961 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1021 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1081 ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1141 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1201 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1261 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1321 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1381 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1441 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1501 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1561 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     1621 tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2281 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat
     2401 tct
//
