LOCUS       sequence-173518-        9365 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Kesarwani AK, Ramirez O, Gupta AK, Yang X, Murthy T, Minella AC, 
            Pillai MM
  TITLE     Cancer-associated SF3B1 mutants recognize otherwise inaccessible 
            cryptic 3' splice sites within RNA secondary structures.
  JOURNAL   Oncogene. 2016 Aug 15. doi: 10.1038/onc.2016.279.
  PUBMED    27524419
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2016 Aug 15. doi: 10.1038/onc.2016.279."
COMMENT     Sequence Label: pCDNA3.1-FLAG-hSF3B1-K700E
FEATURES             Location/Qualifiers
     source          1..9365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..233
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(242..670)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(352..373)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     564..583
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    716..940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     929..946
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4938..4961)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5020..5039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5021..5039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5084..5287)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5113..5133)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5288..5667
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5839..5858
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5933..6037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6038..6106,6107..6898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6256..6275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7069..7657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7558..7577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7811..7828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7945..7966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7981..7986,7987..8004,8005..8011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8019..8035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8024..8046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8043..8059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8043..8059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8108..8229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8119..8138)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8173..8192
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8403..9197)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8514..8533)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9124..9143
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(9264..9365,1..228))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9278..9365,1..48)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9332..9351)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
       61 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      121 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
      181 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat
      241 taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
      301 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
      361 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
      421 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
      481 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
      541 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
      601 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
      661 cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata
      721 gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
      781 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
      841 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
      901 gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt
      961 taaacgggcc ctctagactc gagcggccgc cactgtgctg gatatctgca gaattctcac
     1021 aggatgtagt ccagttcgta tcggatgtaa gtatttttgt cgtcattgta gattctgggg
     1081 taatgggcaa tcagtgcgtc ctgtgagccg atgtagatac tgttgtagat tttccagtag
     1141 acatcccgca cttttcgggc tgggtgaaac aggccctgga gacaatactg gagcatcctg
     1201 caaggtccaa tggccactct caggccctcc agtgctccca tgacagcctg gatcacatga
     1261 ggagaggttt caaagacgtt gggccacacg taattcagca ggtggttcag ggagtcctcg
     1321 catccgaacc catacacgcc caggctcata tgctggacca cagctgaggc agtctgtcgg
     1381 tgcaccaggt cgcgatccat cagggcgtcc tccagcagag gtgtcactgc gtagatgtaa
     1441 tctttcccca tttcgccgat gtactcaaac aggaagctca gtgacttcag gaccccattc
     1501 tgcacgttca gttcgggcac cctatactca ttcatcagag caggcaggac tgtgaagggg
     1561 gaacaggtct cggccacgat ggcgatggcg actgtggtgc acacgcggtt ctgcctctcc
     1621 tggactttca gattgttcag cagggtggcc agcacgtcat gtggtccaat ggcctttgcg
     1681 atgtacccaa aagtattcac tgtggccctt ctaattgctt tcttgtgagc cttcagcagt
     1741 tccagcagct cgaaacagat gcgcatccac tcccgggcgc tcacgtactc tgctcctctg
     1801 tctgcaatcc ggccgaccag atcgatgcaa ttttcctgca ccttctcatg gcggtttttc
     1861 agaatggggg tcagccttgg cagcaggtcc ttgatgggtg gagtcatttt gtgcatccca
     1921 atgacgttca cgatagcctt cagggccccc aggatggagc ccagcacctc agggtattcc
     1981 tcgcccagat attcgtacag gaccactccc aggtgcccca tcagcttttc ctcctgacag
     2041 gttttcatga ccacggcagt ccttgaaatc agatcagcgg cctgctgtct caccttagca
     2101 ctcttgttgt tcagccgcca caggactgtg ccgcaaatct ggggcaggta tggcttcact
     2161 ctttttccca gggcattgac cacggtgcca aatccgttca gcatcacgct gtcttcagtt
     2221 gtctgctcct ggaaggcata cagaatgcca tcgatcagct gttcctccag cttgtggtca
     2281 atgtcggcgg cgcccaggtt tcccataatt ttttcgatgg tctccatgac catcttccgg
     2341 tactgttcgg cctcgtcttt caggtcatcg acgatgcggc taatgatctc ggctgctccg
     2401 accttattgg ccagttcgac ggtagtgtcc accagctggc ggtagttccg tcgatccaga
     2461 gccatcctat gctgccaaaa gtgtttaaag aaagggggca gaatttctgt cttgatataa
     2521 ttggcctcca ctccgtcggt gccacagcac tgcttgacca ctttcaggac gattttcttc
     2581 atttcctcat cagggctctg gaactctcga atcaggatca gcatcacttc gcgggtatag
     2641 tagtttgcgt actcagcatc catcagggga atcagatagc cgatggcctt cagaaaagct
     2701 gccagtcctt tgcctctgtg ctgccggatg cccttccaca ggggtttcag cacggagtcg
     2761 aagctctcga tcccgtatgg tgttgcagcc tctgccaggg ctgcaatagc cagggcggag
     2821 atggtcctga cctcctgctg ctcatccacc aggccatgtt caatgatctc caccaggctt
     2881 ctcaggtggg gcaggatggc acaccccatc agaattgcga tctgctgcac aatcttgatg
     2941 cctgtgtgcc gtgcctgcca tgatttctta ctcttgcaca cggctttcag aaaaggcagc
     3001 agactgggga tgcccagtgc agaagcgacc acggcgaatg ccctagctgt ggtatttctc
     3061 acatactcgt ccatgttgtc aatatctggc cgcattgtac tgatcatggt agccagtcca
     3121 gcggccttgg ccaggttaga aatgatttct cgcccctcca cgcgggcata gtagtcttca
     3181 tcgatcagca gaggctcaat gaccaccagg atcttatgga cgtaggggcg caccaggtca
     3241 tccagcttat acaggattct gtcgatgact ttcaccagca ggtgccgttc ctgatcctcc
     3301 agggttgggg acatcagcag aggcagaatc tgattaaaca ggggtccggc cccgaactcc
     3361 cgtgctttgt cagtgatctg ccgcagtgca gcctttcgca ttggaggtgt gccgttcttg
     3421 atcttcagca gcagcttcat gattttgcgc tccttctgtt cctctggaga cagtgtggat
     3481 tcgtccacgt ccaccagcag cttatcaaag tactggatgt catctggctt caggaaaggc
     3541 agatttccgg aaggctggtc gttcacgctc ttcatagtgc gatcctctgt ctgcatgtga
     3601 aacccggtca ttccccccag aggagtgggt gtagcggtca gtttcctggc gggagttctg
     3661 attgggacgt agccggcagg tggaggcagc accttatagc cctctgggaa catagcgtcc
     3721 agttcctcat cactcagagg tcgattgcgc tcgtcgattt ctcgctccca tcgccaggcc
     3781 tggagctgtt ctggggtcat agacataata tggcctggag taggtgtagc catgttcatg
     3841 gcaggggtgc cgatgggagt ctttcctggt gtcagcacag gggtagagcc tcccatctgg
     3901 gatgcgggtg tctcatccca tctgcttttt ctctttgaag ctccgggggt tggagtctcc
     3961 ccgatgctgt cccctcctcg atcggttcgt ggagtttcgg cccagcctga tccgtgccct
     4021 ggagtgtccc tttctgtctt aggggtctca tcccagcgat ttttccttgc ggagcttgta
     4081 gctcctccat gtcctggagt tgcgtgtcct ggtgtgtcgc ctcttcctgg ggttgctgcg
     4141 ccagctggag tatgagaagg tgtggggtcc caaatcttgc tgcctggggt agctcctgga
     4201 gtctcggagc cctttgctcg tccaggggtt tcgtcccatc gcaggctagg tgtgtgtcct
     4261 ggggtctctg cctgatccca tgaactcagt ttctttggag ttgctccagg tgtctggtca
     4321 gcggtctgat cccagcgcct tttcctctta ctgggtggct gagaagcggc tgcgccattg
     4381 acgactttca gttctccggc ttttgccttc tcggccagct gctgtctgat ctcccgttcc
     4441 tccttggtca gatgctgttc gcgcatcacg tccatgtaag tcctggcgtt catctttgga
     4501 tcaggtgttt tgcctccgtc ggcgaagggg tccagccgct ctggagaaat gatcattgtt
     4561 ctccgatgtt tcttgtattc gtcctctcta tctgcaatct tagggggccg gtgttcagcg
     4621 aaggggtcat actgctcggt ggactgtggg atatcgttca gcagggccac tggtgcgtgg
     4681 tatccaggtt tcttctgccc cagcagtgaa gtagaggagc tatagtcatc gtcatcgtct
     4741 tccagctcgg tagcggcgat gctggtcacg tagccggcga atctactgtc agacccgccg
     4801 tagatttcct ggtcatagta gccggtgcta tccagcccca cgccctgtgc ctcgtccagt
     4861 gctgccttct tgccctgaat ctcccgaatc tgtgcctcaa tgtcctcgtg ggttttagcg
     4921 atcttagcca ttctagactt gtcgtcgtca tctttgtagt ccatggtgga tccgagctcg
     4981 gtaccaagct taagtttaaa cgctagccag cttgggtctc cctatagtga gtcgtattaa
     5041 tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga
     5101 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     5161 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     5221 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     5281 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     5341 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     5401 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     5461 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     5521 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     5581 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     5641 attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca
     5701 gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg
     5761 ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca
     5821 gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat
     5881 cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5941 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6001 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6061 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6241 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6421 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6901 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7741 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7801 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7861 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7921 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7981 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8041 cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg acggtataca
     8101 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     8161 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     8221 aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     8281 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     8341 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     8401 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     8461 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     8521 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     8581 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     8641 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
     8701 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     8761 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     8821 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     8881 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     8941 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     9001 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     9061 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     9121 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     9181 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tccgaaaatg
     9241 gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     9301 tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat aaaaaaaatt
     9361 agtca
//
