LOCUS       sequence-17358-e         961 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, 
            Buchberg AM, Largman C
  TITLE     AbdB-like Hox proteins stabilize DNA binding by the Meis1 
            homeodomain proteins.
  JOURNAL   Mol Cell Biol 1997 Nov;17(11):6448-58.
  PUBMED    9343407
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1997-11"; volume: "17(11)"; pages: "6448-58"
COMMENT     Sequence Label: HOXA7 (murine) His-tag pET
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             104..136
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggcta gcatgactgg
      121 tggacagcaa atgggtcgcg gatccatgag ttcttcgtat tatgtgaacg cgctttttag
      181 caaatatacg gcgggggctt ctctcttcca aaatgccgag ccgacttctt gctcctttgc
      241 acccaactcg cagagaagcg gctacgggcc gggcgccggc gccttcgcct ccactgtgcc
      301 gggcttatac aatgtcaaca gccccctcta tcagagcccc ttcgcgtccg gctatggcct
      361 gggagccgac gcctacaacc tgccctgcgc ctcctacgac caaaacatcc ccgggctctg
      421 cagtgacctc gccaaaggcg cctgccaagg cggacgaggg cgtgcttcac ggcccggccg
      481 aagccagttt ccgcatctac ccctggatgc gcagttcagg acccgacagg aagcggggac
      541 gccagaccta cacgcgctac cagacgctgg aactggagaa ggaattccat ttcaaccgct
      601 acctgacgcg gcgccgccgc atcgagatcg ctcacgcgct ctgcctcact gagcgccaga
      661 tcaagatctg gttccagaat cggcgcatga agtggaagaa agagcataaa gatgagagcc
      721 aggctcccac tgcagccccg gaagacgcgg tgccctccgt ttccacagct gctgacaagg
      781 cggacgagga ggaagaggag gaagaggagg aagaagagga ggaagaggag taaagggcca
      841 ggcacaggac cctggctgca caggacagtt ggaaaagcgt ctttaagaga ctcattgatt
      901 ttagttacaa aaatgggggg aaataaagtg tacggaattc gatatcaagc ttgcgcgatc
      961 a
//
