LOCUS       sequence-173990-       16187 bp    DNA     circular SYN 14-APR-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16187)
  AUTHORS   Vinatzer BA, Teitzel GM, Lee MW, Jelenska J, Hotton S, Fairfax K, 
            Jenrette J, Greenberg JT
  TITLE     The type III effector repertoire of Pseudomonas syringae pv. 
            syringae B728a and its role in survival and disease on host and 
            non-host plants.
  JOURNAL   Mol Microbiol. 2006 Oct;62(1):26-44. Epub 2006 Aug 30.
  PUBMED    16942603
REFERENCE   2  (bases 1 to 16187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol."; date: "2006-10"; volume: "62(1)"; pages: "26-44. Epub 
            2006 Aug 30"
COMMENT     Sequence Label: pBAV150
FEATURES             Location/Qualifiers
     source          1..16187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1108..1341
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        2633..2812
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2854..3405
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLHNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3425..3677
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(4200..4913,4914..4916))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(4203..4913,4914..4916))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4245..4266)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4594..4613
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4853..4874
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    4921..5045
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5086..5391)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5188..5207)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5733..6392)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6307..6326
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(6446..6452,6453..6470,6471..6476))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(6501..6625)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6669..6715)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(6925..6942)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6925..6941)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6934..6956)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7081..7100)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7291..7312
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    7559..7583
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9179..10327)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10626..11420)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11612..12379
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      13002..13713
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(13840..14211)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            14244..14353
                     /note="incP origin of transfer"
     primer_bind     14576..14595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14829..14846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(15034..15684)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    16149..16173
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cctgcaggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggactctagt ttaaacaatg gcagatccaa tgaagctact gtcttctatc
      901 gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag
      961 tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac caaaaggtct
     1021 ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta
     1081 tttctactga tttttcctag gtcgagcgcc cccccgaccg atgtcagcct gggggacgag
     1141 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
     1201 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
     1261 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
     1321 ggaattgacg agtacggtgg ggatccaatt cagcaagcca ctgcaggagt ctcacaagac
     1381 acttcggaaa atcctaacaa aacaatagtt cctgctgcat taccacagct cacccctacc
     1441 ttggtgtcac tgctggaggt gattgaaccc gaggtgttgt atgcaggata tgatagctct
     1501 gttccagatt cagcatggag aattatgacc acactcaaca tgttaggtgg gcgtcaagtg
     1561 attgcagcag tgaaatgggc aaaggcgata ccaggcttca gaaacttaca cctggatgac
     1621 caaatgaccc tgctacagta ctcatggatg tttctcatgg catttgccct gggttggaga
     1681 tcatacagac aatcaagtgg aaacctgctc tgctttgctc ctgatctgat tattaatgag
     1741 cagagaatgt ctctaccctg catgtatgac caatgtaaac acatgctgtt tgtctcctct
     1801 gaattacaaa gattgcaggt atcctatgaa gagtatctct gtatgaaaac cttactgctt
     1861 ctctcctcag ttcctaagga aggtctgaag agccaagagt tatttgatga gattcgaatg
     1921 acttatatca aagagctagg aaaagccatc gtcaaaaggg aagggaactc cagtcagaac
     1981 tggcaacggt tttaccaact gacaaagctt ctggactcca tgcatgaggt ggttgagaat
     2041 ctccttacct actgcttcca gacatttttg gataagacca tgagtattga attcccagag
     2101 atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaaaag
     2161 cttctgtttc atcaaaaatg actcgaccta actgagtaag ctagcttgtt cgagtattat
     2221 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     2281 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     2341 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     2401 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     2461 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactagatcc ccaaacaagc
     2521 ttgaaactga aggcgggaaa cgacaatctg atcatgagcg gagaattaag ggagtcacgt
     2581 tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca
     2641 acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag cacatacgtc
     2701 agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag caaatatttc
     2761 ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat tagagtctca
     2821 tattcactct caactcgatc gaggggatct accatgagcc cagaacgacg cccggccgac
     2881 atccgccgtg ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc
     2941 gagacaagca cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac
     3001 ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc
     3061 ggcatcgcct acgcgggtcc ctggaaggca cgcaacgcct acgactggac ggccgagtcg
     3121 accgtgtacg tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac
     3181 ctgctgaagt ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgcac
     3241 aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg
     3301 gcggccggct tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc
     3361 ctgccggtgc cgccccgtcc ggtcctgccc gtcaccgaaa tctgatgacc cctagagtca
     3421 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     3481 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     3541 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     3601 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     3661 atctatgtta ctagatcggg aattgatccc ccctcgactc ggtaccccct cgacacaaaa
     3721 agcctatact gtacttaact tgattgcata attacttgat catagactca tagtaaactt
     3781 gattacacag ataagtgaag aaacaaacca attcaagaca taaccaaaga gaggtgaaag
     3841 actgttttat atgtctaaca ttgcacctta atatcacact gttagttcct ttcttactta
     3901 aattcaaccc attaaagtaa aaacaacaga taataataat ttgagaatga acaaaaggac
     3961 catatcattt attaactctt atccatccat ttgcattttg atgtccgaaa acaaaaactg
     4021 aaagaacaca gtaaattaca agcagaacaa atgatagaag aaaacagctt ttccaatgcc
     4081 ataatactca aacttagtag gattctggtg tgtgggcaat gaaactgatg cattgaactt
     4141 gacgaacgtt gtcgaaaccg atgatacgga cgaaagctgg gaggcctgga tcgactagtt
     4201 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     4261 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     4321 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     4381 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     4441 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     4501 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     4561 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     4621 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     4681 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     4741 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     4801 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     4861 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcacctca
     4921 accactttgt acaagaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     4981 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     5041 ctatggtcga cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa
     5101 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
     5161 cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
     5221 cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact
     5281 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
     5341 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc
     5401 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc
     5461 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg
     5521 ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc
     5581 gctgtcaact gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg
     5641 gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt
     5701 tatctggctt ttagtaagcc ggatccagat ctttacgccc cgccctgcca ctcatcgcag
     5761 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     5821 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     5881 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     5941 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     6001 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     6061 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     6121 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
     6181 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     6241 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     6301 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     6361 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     6421 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
     6481 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
     6541 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     6601 gttcagcttt tttgtacaaa cttgtgggga gcttgttctc gaggctagag tcgactagct
     6661 tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaaga
     6721 tctcgatcga ggtcgacaga tccccgggta ccgagctcga attcggaggg ctgtcacccg
     6781 tcggagggct gtcacccgtc ggagggctgt cacccggatc ctctagaatt cggagggctg
     6841 tcacccgtcg gagggctgtc acccgtcgga gggctgtcac ccggatcctc tagagtcgac
     6901 cggcatgcaa gcttcgagga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6961 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7021 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgcccg
     7081 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7141 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7201 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7261 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7321 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac
     7381 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     7441 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     7501 caccccagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     7561 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
     7621 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
     7681 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
     7741 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     7801 gagcgttgct gcctgtgatc aaatatcatc tccctcgcag agatccgaat tatcagcctt
     7861 cttattcatt tctcgcttaa ccgtgacagg ctgtcgatct tgagaactat gccgacataa
     7921 taggaaatcg ctggataaag ccgctgagga agctgagtgg cgctatttct ttagaagtga
     7981 acgttgacga tatcaactcc cctatccatt gctcaccgaa tggtacaggt cggggacccg
     8041 aagttccgac tgtcggcctg atgcatcccc ggctgatcga ccccagatct ggggctgaga
     8101 aagcccagta aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag
     8161 taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt ggttcctgta
     8221 ggcatcggga ttggcggatc aaacactaaa gctactggaa cgagcagaag tcctccggcc
     8281 gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag gttgccactt gcgggtcagc
     8341 acggttccga acgccatgga aaccgccccc gccaggcccg ctgcgacgcc gacaggatct
     8401 agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg cagttccgca aatagccccc
     8461 aggaccgcca tcaatcgtat cgggctacct agcagagcgg cagagatgaa cacgaccatc
     8521 agcggctgca cagcgcctac cgtcgccgcg accccgcccg gcaggcggta gaccgaaata
     8581 aacaacaagc tccagaatag cgaaatatta agtgcgccga ggatgaagat gcgcatccac
     8641 cagattcccg ttggaatctg tcggacgatc atcacgagca ataaacccgc cggcaacgcc
     8701 cgcagcagca taccggcgac ccctcggcct cgctgttcgg gctccacgaa aacgccggac
     8761 agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga cagcccaatg
     8821 atctcgccgt cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc
     8881 tccatgggct ttttctcctc gtgctcgtaa acggacccga acatctctgg agctttcttc
     8941 agggccgaca atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc
     9001 tgagccttaa tcacaattgt caattttaat cctctgttta tcggcagttc gtagagcgcg
     9061 ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct
     9121 gaaatgccag taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct
     9181 agcgtttgca atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc
     9241 gcaactcttc gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg
     9301 atccgcacat gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga
     9361 aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt
     9421 tcgtgtagtg gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac
     9481 gggacgtttt cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca
     9541 ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt
     9601 cctcggcctt cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt
     9661 agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct
     9721 gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt
     9781 ccttgttgac gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc
     9841 gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc
     9901 acggcgcaat atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca
     9961 gcaacgcggc ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc
    10021 gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg
    10081 cctcctgttc gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg
    10141 gagccagttg cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga
    10201 cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat
    10261 cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc
    10321 gattcattca ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt
    10381 cctgatttga cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt
    10441 cggtcccgta gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca
    10501 cgaataccag cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac
    10561 acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct
    10621 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
    10681 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
    10741 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
    10801 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
    10861 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
    10921 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
    10981 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
    11041 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
    11101 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
    11161 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
    11221 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
    11281 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
    11341 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
    11401 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
    11461 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
    11521 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
    11581 cgacggagcc gattttgaaa ccacaattat gggtgatgct gccaacttac tgatttagtg
    11641 tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt
    11701 cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct
    11761 gctctcactg ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta
    11821 cgcaccagaa aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca
    11881 ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt
    11941 aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg
    12001 gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acagtctccg
    12061 gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg ggcgtcttat
    12121 gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga
    12181 atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg
    12241 gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc
    12301 aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca
    12361 ataagttgga gtcattaccc aattatgata gaatttacaa gctataaggt tattgtcctg
    12421 ggtttcaagc attagtccat gcaagttttt atgctttgcc cattctatag atatattgat
    12481 aagcgcgctg cctatgcctt gccccctgaa atccttacat acggcgatat cttctatata
    12541 aaagatatat tatcttatca gtattgtcaa tatattcaag gcaatctgcc tcctcatcct
    12601 cttcatcctc ttcgtcttgg tagcttttta aatatggcgc ttcatagagt aattctgtaa
    12661 aggtccaatt ctcgttttca tacctcggta taatcttacc tatcacctca aatggttcgc
    12721 tgggtttatc gcacccccga acacgagcac ggcacccgcg accactatgc caagaatgcc
    12781 caaggtaaaa attgccggcc ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa
    12841 gggcaggccg ccacccaggc cgccgccctc actgcccggc acctggtcgc tgaatgtcga
    12901 tgccagcacc tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc
    12961 cgttactgcc ccgatcccgg caatggcaag gactgccagc gctgccattt ttggggtgag
    13021 gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcgggccg
    13081 ggagggttcg agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc
    13141 agccctggtt aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt
    13201 agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca
    13261 gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca
    13321 aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac
    13381 ttatccccag gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc
    13441 cgatttgcga ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg
    13501 tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag
    13561 tgagggccaa gttttccgcg aggtatccac aacgccggcg gccgcggtgt ctcgcacacg
    13621 gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga
    13681 gggcaaccag cccggtgagc gtcggaaagg cgctcggtct tgccttgctc gtcggtgatg
    13741 tacttcacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca
    13801 atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg
    13861 gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag
    13921 ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt
    13981 cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc
    14041 atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt aggccgacag
    14101 gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg gaaggcagta
    14161 caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc
    14221 ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac
    14281 cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc
    14341 acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg
    14401 gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac
    14461 cccgaagcag ggttatgcag cggaaaagcg ccacgcttcc cgaagggaga aaggcggaca
    14521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    14581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    14641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    14701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    14761 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    14821 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg ccagaaggcc gccagagagg
    14881 ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct
    14941 gctacgggcg tctgacgcgg tggaaagggg gaggggatgt tgtctacatg gctctgctgt
    15001 agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc
    15061 aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg
    15121 ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg
    15181 cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg
    15241 ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc
    15301 cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg
    15361 cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc
    15421 ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg
    15481 caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg
    15541 cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt
    15601 gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac
    15661 tgtattcggc tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc
    15721 accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg
    15781 aggccagacg tgaaacccaa catacccctg atcgtaattc tgagcactgt cgcgctcgac
    15841 gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac
    15901 tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt
    15961 gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctc
    16021 gtctcgctgg ccggcgccag atctggggaa ccctgtggtt ggcatgcaca tacaaatgga
    16081 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
    16141 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagttt
//
