LOCUS       sequence-174156-       10714 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Early endosome-targeted mCherry-tagged recruiter construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I
  TITLE     Localization of mTORC2 activity inside cells.
  JOURNAL   J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 
            10.1083/jcb.201610060.
  PUBMED    28143890
REFERENCE   2  (bases 1 to 10714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060."
COMMENT     Sequence Label: pHR-FKBP:mCherry-Rab5a
FEATURES             Location/Qualifiers
     source          1..10714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             89..322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(347..365)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(348..365)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(655..674)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(888..907)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1007..1029
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1067..1085)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1153..1257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1258..1326,1327..2118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1476..1495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2289..2877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2778..2797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3031..3048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3118..3138)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3123..3452
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3303..3438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4586..4651
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4781..4801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5226..5360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5263..5282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5317..5336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5528..6161
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6208..6333
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6830..7063
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7248..7292
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7577..7694
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7847..8254
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8288..8297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8294..8296,8297..8617)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             8642..9349
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8787..8805)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9055..9074
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      9359..9368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9371..9397
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    10105..10693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10158..10178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10576..10587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10696..10712)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10697..10713)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
       61 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
      121 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      181 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      241 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      301 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc
      361 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
      421 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
      481 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
      541 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
      601 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      661 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      721 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
      781 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
      841 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
      901 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      961 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     1021 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     1081 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1141 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1201 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1261 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1321 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1381 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1441 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1501 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1561 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1621 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1681 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1741 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1801 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1861 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1921 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1981 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2041 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2101 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2161 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2221 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2401 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2941 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3001 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3061 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga
     3121 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3181 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3241 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3301 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3361 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3421 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag
     3481 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
     3541 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
     3601 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
     3661 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
     3721 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg
     3781 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     3841 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     3901 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
     3961 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
     4021 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
     4081 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
     4141 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
     4201 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     4261 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
     4321 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
     4381 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt
     4441 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag
     4501 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     4561 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     4621 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     4681 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     4741 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     4801 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     4861 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     4921 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     4981 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     5041 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     5101 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     5161 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5221 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5281 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5341 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     5401 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     5461 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     5521 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     5581 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     5641 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     5701 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     5761 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     5821 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     5881 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     5941 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     6001 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6061 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6121 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     6181 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     6241 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     6301 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     6361 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     6421 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     6481 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     6541 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     6601 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     6661 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     6721 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     6781 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     6841 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     6901 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     6961 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     7021 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     7081 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     7141 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     7201 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     7261 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     7321 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     7381 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     7441 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     7501 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg
     7561 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     7621 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     7681 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc
     7741 ttgatatcga attcctgcag ccccgataaa ataaaagatt ttatttagtc tccagaaaaa
     7801 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gctgcagtaa cgccattttg
     7861 caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg
     7921 aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg ccccggcccg
     7981 gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt
     8041 ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc
     8101 cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc
     8161 ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg
     8221 cgcgccagtc ctccgacaga ctgagtcgcc cgggggggat ctggagctct cgagaattct
     8281 cacgcgtgcc accatgggag tgcaggtgga gactatctcc ccaggagacg ggcgcacctt
     8341 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa
     8401 atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt
     8461 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac
     8521 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc
     8581 cactctcgtc ttcgatgtgg agcttctaaa actggaaggt gctgcacttc tgcccggttc
     8641 tatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     8701 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     8761 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     8821 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     8881 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     8941 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     9001 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     9061 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     9121 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     9181 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     9241 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     9301 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagc ccggttctgc
     9361 cgccatggca tatccatatg acgtcccaga ctatgctggc gctagtcgag gcgcaacaag
     9421 acccaacggg ccaaatactg gaaataaaat atgccagttc aaactagtac ttctgggaga
     9481 gtctgctgtt ggcaaatcaa gcctagtgct tcgttttgtg aagggccaat ttcatgaatt
     9541 tcaagagagt accatagggg ctgcttttct aacccaaact gtgtgtcttg atgatacaac
     9601 agtaaagttt gaaatatggg atacagctgg tcaagaacga taccatagct tagcaccaat
     9661 gtactacaga ggagcacaag cagccatagt tgtatatgat atcacaaatg aggagtcctt
     9721 tgccagagcc aaaaactggg ttaaagaact tcagaggcaa gccagtccta acattgtaat
     9781 agctttatca ggaaacaagg ctgatcttgc aaataaaaga gctgtcgatt tccaggaagc
     9841 acagtcctat gcagatgaca acagtttatt attcatggag acatcagcta aaacatcgat
     9901 gaatgtaaat gaaatattca tggcaatagc taaaaagttg ccaaagaacg aaccacagaa
     9961 tccaggagca aattctgcca gaggaagagg agtagacctt actgaaccca cgcagccaac
    10021 caggaatcag tgttgtagta actaagcggc cgcgactcta gagtcgacct gcaggcatgc
    10081 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
    10141 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    10201 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    10261 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    10321 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    10381 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    10441 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    10501 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    10561 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    10621 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    10681 gccgcctccc cgcatcgata ccgtcgacct cgag
//
