LOCUS       sequence-174279-        3563 bp    DNA     circular SYN 15-APR-2025
DEFINITION  In situ probe for mouse Nrg3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Bartolini G, Sanchez-Alcaniz JA, Osorio C, Valiente M, 
            Garcia-Frigola C, Marin O
  TITLE     Neuregulin 3 Mediates Cortical Plate Invasion and Laminar Allocation
            of GABAergic Interneurons.
  JOURNAL   Cell Rep. 2017 Jan 31;18(5):1157-1170. doi: 
            10.1016/j.celrep.2016.12.089.
  PUBMED    28147272
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.12"; journalName: "Cell Rep"; date: 
            "2017-01-31- 31"; volume: "18"; issue: "5"; pages: "1157-1170"
COMMENT     Sequence Label: pBluescript-KS-Nrg3-ISH
FEATURES             Location/Qualifiers
     source          1..3563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     680..696
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     680..696
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1378..1398)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1378..1396)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1417..1433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1417..1433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1430..1452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1441..1457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1465..1471,1472..1489,1490..1495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1510..1531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1648..1665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1819..2407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1899..1918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2578..3369,3370..3438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3201..3220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3439..3543)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct
      661 ccaccgcggt ggcggccgct ctagaactag tggatccccc gggctgcagg aattcgatat
      721 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcggct
      781 tttcgaattc acacaggcca tgccgtcgtg gcccactgcg gcgtatgcta cctcctccta
      841 cctccacgat tccactccct cctggaccct gtcacccttt caggatgctg ctgccgcctc
      901 ttcctcctca ccctcttcca cctcctccac taccaccacc ccagaaacta gcaccagccc
      961 caaatttcat actacaacat actccactga acgatctgag cacttcaaac cctgtcgaga
     1021 caaggacctg gcgtattgtc tcaatgatgg tgaatgcttt gtgattgaga ccctgacagg
     1081 atcccataag cactgtcggt gcaaggaagg ctaccaagga gtccgttgtg atcaatttct
     1141 gccgaaaaca gactccatct tatcggatcc aacagaccac ttggggattg aattcatgga
     1201 gagtgaagac gtttatcaaa ggcaggtgct gtcaatttca tgtatcatct ttggaattgt
     1261 catcgtgggc atgttctgcg cggctttcta ttttggatcc gaagaaaagc cgaattctgc
     1321 agatatccat cacactggcg gccgctcgag ggggggcccg gtacccagct tttgttccct
     1381 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1441 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1501 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1561 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1621 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1681 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1741 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1801 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1861 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1921 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1981 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2041 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2101 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2161 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2221 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2281 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2341 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2401 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2461 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2521 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2581 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2641 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2701 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2761 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2821 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2881 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2941 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3001 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3061 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3121 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3181 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3241 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3301 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3361 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3421 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3481 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3541 gcgcacattt ccccgaaaag tgc
//
