LOCUS       sequence-174440-        8816 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses an inducible short hairpin targeting human MKP1 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: tet-pLKO shMKP1.v2 puro
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    901..922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(937..942,943..960,961..967)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    975..991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     980..1002
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     999..1015
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     999..1015
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1034..1054
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1036..1054
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1082..1308
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1309..1489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1536..1661
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2154..2387
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2572..2616
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2905..2999
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2916..2935
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2953..2971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2981..2999
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3100..3217
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3266..3776
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3695..3713
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3785..4357
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4365..4384
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4412..4430
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4441..5061
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4514..4533)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5096..5669
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5263..5280)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5490..5509
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5689..6288
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5689..5708)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6185..6205
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6416..6649
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6721..6842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6758..6777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6812..6831
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6882..7017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7038..7056)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7066..7083)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7066..7082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7075..7097)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7224..7679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7311..7330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7521..7542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7705..7809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7810..7878,7879..8670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8028..8047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      841 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      901 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      961 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1021 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     1081 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     1141 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     1201 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     1261 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     1321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1501 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1561 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1621 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1681 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1741 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1801 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1861 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1921 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1981 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2041 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     2101 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2161 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2221 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2281 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2341 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2401 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2461 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2521 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2581 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2641 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2701 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2761 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2821 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     2881 cgagactagc ctcgagcggc cgcaatattt gcatgtcgct atgtgttctg ggaaatcacc
     2941 ataaacgtga aatccctatc agtgatagag acttataagt tccctatcag tgatagagac
     3001 accggtcgag agggctggtc cttattctgc agaataagga ccagccctct cgatttttga
     3061 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     3121 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     3181 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     3241 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
     3301 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     3361 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     3421 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     3481 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     3541 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     3601 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     3661 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     3721 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     3781 atctgtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
     3841 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
     3901 ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc
     3961 tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta
     4021 acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact
     4081 tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat
     4141 tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa
     4201 tattcttatt ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt
     4261 acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc
     4321 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     4381 tgtgctgtct catcattttg gcaaagaatt gtaatacgac tcactatagg gcgaattgat
     4441 atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
     4501 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
     4561 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
     4621 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
     4681 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
     4741 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
     4801 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
     4861 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
     4921 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
     4981 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
     5041 cttaaatgtg aaagtgggtc cgcgtacagc ggctcccggg agttctaggg atctgcccct
     5101 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5161 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5221 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5281 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5341 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5401 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5461 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5521 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5581 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5641 tggttttcct ttgaaaaaca cgatgataag gatccaccgg agcttaccat gaccgagtac
     5701 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5761 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5821 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5881 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5941 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6001 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6061 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6121 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6181 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6241 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
     6301 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca
     6361 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6421 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6661 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6721 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6781 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6841 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6901 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6961 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7021 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     7081 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     7141 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7201 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7261 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7321 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7381 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7441 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7501 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7561 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7621 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     7681 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7741 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7801 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7861 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7921 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7981 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8041 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8101 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8161 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8221 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8281 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8341 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8401 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8461 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8521 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8581 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8641 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8701 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8761 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccc
//
