LOCUS       sequence-175056-        5757 bp    DNA     circular SYN 14-APR-2025
DEFINITION  p15A plasmid expressing CasX locus from Deltaproteobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Burstein D, Harrington LB, Strutt SC, Probst AJ, Anantharaman K, 
            Thomas BC, Doudna JA, Banfield JF
  TITLE     New CRISPR-Cas systems from uncultivated microbes.
  JOURNAL   Nature. 2016 Dec 22. doi: 10.1038/nature21059.
  PUBMED    28005056
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Dec 22. doi: 10.1038/nature21059."
COMMENT     Sequence Label: pCasX1
FEATURES             Location/Qualifiers
     source          1..5757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      407..434
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(462..479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(596..1141)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(676..695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1255..1349)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1370..2029)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1944..1963
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2030..2132)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tgtaaaaggg tttacagtgc agatctcctt ataattattg aaaatgtgtt tcgttactct
       61 taatattcga gaatttcgac ttccggaact cattgatata tctgggttgt tggtatttga
      121 aggtatctcc gataagtaaa acgcatcaaa gtattaaata ctcgtattgc tgttcgatta
      181 tatttgaagg tatctccgat aagtaaaacg catcaaaggc cgctctgaaa aaggaaaagc
      241 tcggactaaa attatatttg ggcgggaagc aacgtaaagc cttcttttct tgctgcatct
      301 ctcagttttg aatcaaggca gacgaaataa tgaccttttg gcctttttcc tgcccatacg
      361 agcgcggcag atagttgcaa ggcatctgcg gcactagagt cacactggct caccttcggg
      421 tgggcctttc tgcgtttata cctagggata tattccgctt cctcgctcac tgactcgcta
      481 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      541 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      601 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      661 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      721 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      781 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      841 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      901 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      961 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
     1021 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
     1081 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     1141 agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc
     1201 aaatgtagca cctgaagtca gccccatacg atataagttg ttactagtgc ttggattctc
     1261 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1321 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1381 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1441 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1501 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1561 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     1621 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1681 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1741 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1801 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1861 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1921 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     1981 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     2041 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     2101 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag atatcgacgc
     2161 aatgtattat gcagcacatg cccctttaaa atcagaagga attgatgtca tcaagcactc
     2221 agccgttgaa tcagccttcg ggtattactt tgcgaagact ggaaagatta atcccaaaca
     2281 ccacaggatg ctaatagacg caagaaagat tcgtgaaata gccgattatg atattcagga
     2341 agagattgtt gagccaactg catcgctaaa aattaaagag gggaagtctt ttttgtctgc
     2401 aatcagaaaa attcttggca gcctgtagca atggacttga caagcgaagt gggactgtcc
     2461 aggatttacg gacagagcga agcggagtcc cgaatccaat ggttgtggtt tgcgggcagg
     2521 cattattttc gttttataat ctggaaagaa aaaaggaaaa ccccttatgg agaagcgtat
     2581 taacaagatt cgtaagaagt tatcggctga taatgccaca aaacctgtaa gccgctcagg
     2641 acctatgaaa acgcttttag ttcgtgttat gacagatgat ctgaaaaaac gtcttgagaa
     2701 gcgtcgtaaa aagccggagg tcatgcctca agtcatttcg aataatgccg ccaataattt
     2761 acgtatgctt cttgatgact atacaaagat gaaagaagca atcctgcaag tctactggca
     2821 agagttcaaa gatgatcatg taggtttgat gtgtaagttt gcacagcccg ctagtaaaaa
     2881 gatcgaccaa aacaagttaa agccagagat ggacgagaaa gggaatctta ccactgccgg
     2941 gttcgcttgc tctcaatgcg gccaaccgct gttcgtatat aagctggagc aagtttcaga
     3001 aaagggtaag gcatacacaa attatttcgg acgttgcaac gtcgctgagc acgagaagct
     3061 tattctttta gcccagctga aaccagagaa ggactctgat gaagcggtta cctactcctt
     3121 gggaaagttc ggacagcgcg ctcttgactt ttattccatc cacgtgacaa aggaaagtac
     3181 acaccccgtc aaaccactgg cccaaattgc agggaaccgc tacgcgagcg ggccagtggg
     3241 aaaagcattg tcagacgctt gtatgggcac gattgcttcc tttcttagta aataccagga
     3301 tattatcatt gaacatcaaa aggtagttaa ggggaaccag aagcgtctgg aaagcttacg
     3361 tgaacttgca gggaaagaga accttgagta cccatcggtc acattgcctc cgcagccgca
     3421 cacaaaggag ggtgtcgatg cttataacga ggtgattgca cgcgtacgca tgtgggtaaa
     3481 tttgaatctg tggcagaagt tgaaactgtc gcgtgatgac gccaaacctc ttttacgcct
     3541 gaagggcttc ccctcatttc cagtcgtaga gcgccgtgaa aatgaagtag actggtggaa
     3601 tactatcaac gaagtaaaaa agctgattga cgcgaagcgc gacatgggac gcgtgttttg
     3661 gagcggcgtt actgctgaaa agcgcaacac catccttgaa ggatacaact atttacccaa
     3721 cgagaacgat cataaaaagc gcgagggcag tttagagaat ccgaagaagc cggccaaacg
     3781 tcagtttggc gaccttttat tatacctgga aaagaaatat gcgggtgact ggggcaaagt
     3841 gttcgatgaa gcctgggagc gcattgacaa gaaaatcgct ggtctgacgt cgcacattga
     3901 gcgcgaggaa gctcgtaacg cagaagacgc ccaatccaaa gcggtcctga cagactggct
     3961 gcgcgctaag gcgtcctttg ttcttgagcg tttaaaagag atggacgaaa aggaattcta
     4021 tgcctgtgag attcaattac aaaagtggta tggtgactta cgcgggaacc cctttgctgt
     4081 agaggctgag aatcgtgtag tggacatttc aggtttcagc atcggatcgg atggacattc
     4141 tatccaatat cgtaaccttc ttgcatggaa atatttggag aatggtaagc gcgagttcta
     4201 tctgcttatg aactacggga aaaaaggacg tattcgcttc accgatggta cagacattaa
     4261 aaaaagtggc aaatggcaag ggttgttata tgggggtggc aaagcaaagg ttatcgattt
     4321 aacgtttgat cccgatgatg agcagctgat tatcttgcct ttagctttcg gaacccgcca
     4381 gggccgtgaa tttatctgga acgacttgct ttctcttgag acaggactta tcaaacttgc
     4441 gaatggacgc gtgatcgaaa agactattta caataaaaag atcggtcgcg atgagccggc
     4501 cctgtttgtc gccttaacat tcgagcgtcg cgaagttgtt gatccgtcaa atatcaagcc
     4561 ggtaaactta atcggggtcg atcgtggtga aaatatcccg gcagtaatcg ctcttacaga
     4621 cccagaaggc tgccccttac ccgagttcaa ggactcatcc ggcggtccaa cagacatcct
     4681 tcgcattggc gaaggatata aagagaaaca gcgtgcaatc caggcagcaa aagaagtgga
     4741 acaacgtcgc gccggaggct acagccgcaa atttgcgtca aaatcgcgta acttggcaga
     4801 tgatatggtc cgcaacagcg cccgtgattt gttttaccac gcggtgacac atgacgctgt
     4861 tttagttttt gagaacttgt cacgtgggtt cggacgtcag ggaaaacgca ccttcatgac
     4921 tgagcgccaa tacacaaaaa tggaagactg gttgacggct aaactggctt acgaggggct
     4981 tactagtaaa acgtatttgt cgaaaactct tgctcagtac acctctaaaa cttgttctaa
     5041 ctgcgggttc acgattacca cagcggacta cgatggcatg ctggtacgcc ttaaaaagac
     5101 gagcgacggg tgggcaacga cattgaacaa taaagaactt aaggccgagg gacaaatcac
     5161 ctactataat cgttataagc gccagacggt agaaaaagag ttaagcgccg aacttgaccg
     5221 cctgagcgag gagagtggga acaacgacat ttcaaaatgg actaaggggc gccgtgacga
     5281 ggctttgttt ttgctgaaga aacgtttctc ccatcgtccc gtacaagagc agttcgtttg
     5341 cttagattgt ggccatgaag ttcatgctga tgaacaggct gctttaaata tcgcgcgttc
     5401 ctggttgttt cttaatagta actcaactga gttcaaatca tataagtcag ggaaacagcc
     5461 gtttgtggga gcatggcagg ctttttataa acgccgcctg aaggaggtct ggaagccgaa
     5521 tgcttgatat tgccgataag caccgtattt ttcttgactg caatctgtca taagtagtaa
     5581 attacatctg gcgcgtttat tccattactt tggagccagt cccagcgact atgtcgtatg
     5641 gacgaagcgc ttatttatcg gagatagctc cgggtgcaaa ctcggagctg tttttttacg
     5701 aaacagctaa ttttagccaa aagttctttg aaaacctgat attacggttt ttttgtt
//
