LOCUS       sequence-175183-       12835 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry
            reporter and constitutive tBFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 12835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP
FEATURES             Location/Qualifiers
     source          1..12835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     misc_feature    1561..2134
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1728..1745)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1955..1974
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2153..2863
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2298..2316)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2566..2585
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2948..3435
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2983..3006)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3368..3387
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3456..4157
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4193..4781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4246..4266)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4664..4675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4784..4800)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4785..4801)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4891..5124
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5149..5167)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5150..5167)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5457..5476)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5690..5709)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5809..5831
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5869..5887)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5955..6059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6060..6128,6129..6920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6278..6297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7091..7679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7580..7599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7833..7850
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7920..7940)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7925..8254
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8105..8240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9388..9453
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9583..9603
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10028..10162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10065..10084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10119..10138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10330..10963
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11010..11135
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11632..11865
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12050..12094
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12379..12496
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     12722..12746
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgtatagga tgcagctcct ctcctgcatc gctctgagtt tggccctggt gaccaatagt
       61 gcccaagtaa ttaatacgaa ttcacttagt ttgttgacgc agaacaactt gaacaagagt
      121 caatccgcac tcggaacagc gattgaaagg ctgagttctg ggctgagaat caattccgct
      181 aaggatgacg ctgcaggaca agcgatagca aacaggttca ccgcgaatat caagggcctg
      241 acccaggcat ctcgaaatgc gaacgacggt atcagtatag cgcaaactac tgagggtgcg
      301 ttgaatgaaa tcaacaataa tctccaaaga gtacgcgagc tcgctgtaca gagtgctaat
      361 tctactaata gtcagagcga tctcgacagc atccaagcag agattaccca acggctcaac
      421 gagattgaca gagtgtctgg gcagactcaa tttaatggtg tgaaggtgct cgcgcaagac
      481 aacacactga cgatacaagt gggtgcgaac gacggggaaa ccatagacat cgacttgaaa
      541 caaatcaatt cccaaacact tggactggac acgttgaatg tgcagcaaaa gtataaggtc
      601 tccgatactg ctgcaacggt gacgggatac gccgatacca ctattgcgtt ggataactct
      661 acgtttaaag cgtccgcaac gggacttggc gggactgacc agaaaataga tggcgatttg
      721 aagttcgacg ataccaccgg aaagtattac gccaaagtca cggttactgg gggtaccggt
      781 aaagatggtt actatgaggt tagtgtggat aagacaaacg gggaggttac tctggcaggc
      841 ggcgcgactt caccgctgac aggaggtctg cctgcaacgg cgacggaaga cgttaagaat
      901 gtccaagtag ccaatgcaga tcttactgaa gcgaaagccg cccttaccgc cgccggagtt
      961 accggcacgg cctccgttgt caagatgtca tacacggata ataatggaaa aactatagac
     1021 ggagggcttg ccgtgaaagt tggtgatgat tattattccg caactcagaa taaagatgga
     1081 tccattagca taaacacgac taagtacaca gccgacgatg gaacctctaa gacggcgttg
     1141 aacaagctcg gcggcgctga tggaaagact gaagtagttt ccataggcgg aaaaacttac
     1201 gcggctagta aagctgaggg acataacttt aaagcgcagc ctgaccttgc cgaggcggcg
     1261 gccacaacta cagaaaaccc gctgcagaaa atcgacgctg cattggcaca agtagatacg
     1321 ctgcgcagtg accttggtgc ggtccagaat agattcaaca gtgccattac taaccttggc
     1381 aacacagtta ataatctcac ttccgcacgc tccaggattg aagattctga ctacgctacg
     1441 gaggtcagca acatgtcacg ggctcaaatc cttcagcaag ctgggacttc cgtcttggca
     1501 caggcaaatc aggttccgca gaacgtattg tccctcctta gagatccttg acttgcggcc
     1561 cccctctccc tcccccsccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     1621 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     1681 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     1741 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     1801 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     1861 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     1921 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     1981 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     2041 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     2101 ggacgtggtt ttcctttgaa aaacacgatg ataaatgagc gctggcgggt ccatggtgag
     2161 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     2221 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2281 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     2341 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     2401 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2461 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     2521 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     2581 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     2641 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     2701 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     2761 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     2821 gggccgccac tccaccggcg gcatggacga gctgtacaag tagggccgca actcccacct
     2881 gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcacga ggttaacgaa
     2941 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     3001 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     3061 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     3121 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     3181 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     3241 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     3301 ggaaggggtg ggtccggggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     3361 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     3421 tctccgggcc tttcgacctg cagcccaagc ttaccatgag cgagctgatt aaggagaaca
     3481 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     3541 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     3601 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     3661 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     3721 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     3781 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     3841 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     3901 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     3961 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     4021 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     4081 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     4141 ggcacaagct taattaatgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     4201 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4261 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4321 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4381 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4441 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4501 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4561 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4621 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4681 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4741 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     4801 agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4861 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4921 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4981 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5041 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5101 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     5161 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     5221 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     5281 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     5341 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     5401 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     5461 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     5521 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     5581 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     5641 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     5701 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5761 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5821 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5881 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5941 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6001 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6061 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6121 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6181 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6241 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6301 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6361 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6421 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6481 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6541 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6601 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6661 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6721 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6781 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6841 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6901 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6961 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7021 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7081 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7141 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7201 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7261 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7321 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7381 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7441 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7501 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7561 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7621 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7681 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7741 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7801 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7861 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     7921 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7981 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8041 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8101 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8161 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8221 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     8281 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     8341 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     8401 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     8461 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     8521 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     8581 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     8641 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     8701 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     8761 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     8821 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     8881 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     8941 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     9001 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     9061 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     9121 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     9181 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     9241 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     9301 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     9361 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     9421 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     9481 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     9541 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     9601 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     9661 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     9721 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     9781 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     9841 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     9901 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     9961 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10021 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10141 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    10201 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    10261 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10321 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
    10381 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
    10441 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
    10501 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
    10561 atgacccgta gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
    10621 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
    10681 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    10741 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    10801 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    10861 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    10921 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    10981 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    11041 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    11101 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    11161 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    11221 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    11281 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    11341 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    11401 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    11461 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    11521 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11581 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    11641 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11701 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11761 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11821 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11881 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11941 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    12001 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    12061 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    12121 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    12181 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    12241 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    12301 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
    12361 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    12421 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    12481 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
    12541 gcttgatatc gaattcggag cactgtcctc cgaacgtcgg agcactgtcc tccgaacgtc
    12601 ggagcactgt cctccgaacg tcggagcact gtcctccgaa cggagcatgt cctccgaacg
    12661 tcggagcact gtcctccgaa cgactagtta ggcgtgtacg gtgggaggcc tatataagca
    12721 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
    12781 atagaagaca ccgggaccga tccagcctct cgacattcgt tggatcgccg ccacc
//
