LOCUS       sequence-175194-       11498 bp    DNA     circular SYN 14-APR-2025
DEFINITION  [Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with 
            constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 11498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry
FEATURES             Location/Qualifiers
     source          1..11498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             547..600
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        1596..2095
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1631..1654)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2028..2047
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2116..2826
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2529..2548
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2903..3491
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2956..2976)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3374..3385)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3554..3787
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3812..3830)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3813..3830)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4120..4139)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4353..4372)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4472..4494
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4532..4550)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4618..4722
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4723..4791,4792..5583)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4941..4960)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5754..6342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6243..6262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6496..6513
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6583..6603)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6588..6917
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6768..6903
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8051..8116
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8246..8266
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8691..8825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8728..8747)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8782..8801
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8993..9626
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9673..9798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10295..10528
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10713..10757
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11042..11159
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     11385..11409
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgcatagct ctgccctgct gtgctgtctg gtcctcttga caggagtgag ggcttcacct
       61 ggacaaggaa cccagtctga aaacagctgc actcactttc ccggtaatct ccccaatatg
      121 ctgcgggatt tgagggacgc ctttagtagg gtgaagacgt tctttcagat gaaagaccaa
      181 ctcgataact tgctcctgaa agagtcactc ttggaggatt ttaagggtta tcttggttgc
      241 caggcattga gtgagatgat tcagttctat ttggaagaag taatgcctca ggctgagaac
      301 caggatcccg acattaaagc gcacgtaaat tccctcggtg aaaatttgaa gacactcagg
      361 ctccggctgc gacgctgcca ccgctttctt ccgtgcgaaa ataagtctaa agccgtggag
      421 caggtgaaga atgctttcaa taagctccaa gagaaaggca tatataaagc aatgtcagag
      481 tttgacattt tcatcaacta catcgaagcc tacatgacca tgaaaatcag aaatcggaag
      541 cgcagagagg gtcgaggttc cctgctgact tgtggcgacg tagaagagaa tcctgggcct
      601 atgcggattt tcgcggtatt tatattcatg acatactggc accttttgaa tgcattcacc
      661 gttacagtgc cgaaggacct ttacgttgtc gaatatggtt ccaatatgac tatcgagtgt
      721 aagttcccgg ttgagaaaca gctggatctt gcggcgctga ttgtttactg ggaaatggaa
      781 gacaagaaca ttatacagtt cgtccatggg gaggaggatc tcaaagtcca acactcttca
      841 tatcgccagc gggcacggct tttgaaagac caactttctc tcggtaacgc cgctttgcaa
      901 attaccgacg tcaaactcca ggatgcgggc gtatacagat gcatgatttc atatggcgga
      961 gcagattata agcgaatcac ggtcaaagtc aacgccccat ataacaagat caaccaaaga
     1021 atcctggttg ttgacccggt aacaagcgag catgagctga catgccaagc tgaaggatat
     1081 ccaaaagctg aggtaatatg gacaagctca gaccaccagg tactgtcagg taagaccacc
     1141 acaacaaaca gcaaaagaga agagaagctg ttcaacgtta cgtccacact tcggattaat
     1201 acaactacca acgagatctt ctactgcaca ttcaggcgac tcgatcctga agagaaccat
     1261 actgccgaac tggttatacc ggagttgccg cttgctcatc caccgaatga gcgcacgcat
     1321 ctggttatac tgggcgcaat tctcctctgc ctcggggtgg cacttacctt tatctttagg
     1381 ctccgcaaag gacggatgat ggacgttaaa aagtgtggaa ttcaagacac aaatagcaaa
     1441 aagcagagtg atacacacct cgaagaaact taggatcctt gacttgcggc cgcaactccc
     1501 acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc atctaggcgc
     1561 cggaattaga tctctcgagg ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag
     1621 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     1681 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     1741 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     1801 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     1861 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     1921 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     1981 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     2041 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     2101 cagcccaagc ttaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     2161 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     2221 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     2281 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     2341 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     2401 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     2461 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     2521 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     2581 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     2641 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     2701 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     2761 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     2821 aagtaaagcg gccgcgactc tagagtcgac ctgcaggcat gcaagcttgc aggcatgcaa
     2881 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     2941 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3001 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3061 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3121 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3181 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3241 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3301 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3361 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3421 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3481 cgcctccccg caacgccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     3541 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     3601 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3661 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3721 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3781 tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta
     3841 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta
     3901 attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca
     3961 gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa
     4021 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg
     4081 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     4141 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     4201 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     4261 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     4321 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4381 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     4441 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     4501 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4561 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4621 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4681 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4741 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4801 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4861 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4921 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4981 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5041 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5101 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5161 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5221 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5281 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5341 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5401 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5461 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5521 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5581 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5641 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5701 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5761 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5821 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5881 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5941 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6001 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6061 cggtcgggct gaacgggggg ttcatgcaca cagcccagct tggagcgaac gacctacacc
     6121 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6181 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6301 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6361 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6421 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6481 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6541 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
     6601 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6661 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6721 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6781 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6841 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6901 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
     6961 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     7021 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     7081 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     7141 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     7201 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
     7261 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     7321 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     7381 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     7441 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     7501 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     7561 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     7621 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     7681 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     7741 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     7801 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     7861 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     7921 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
     7981 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     8041 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     8101 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     8161 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     8221 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     8281 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     8341 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     8401 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     8461 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     8521 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     8581 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     8641 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     8701 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8761 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8821 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     8881 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     8941 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     9001 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     9061 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     9121 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     9181 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     9241 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     9301 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     9361 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     9421 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     9481 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9541 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9601 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     9661 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     9721 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     9781 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     9841 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     9901 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     9961 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    10021 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    10081 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    10141 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    10201 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    10261 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    10321 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    10381 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    10441 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
    10501 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    10561 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    10621 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    10681 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    10741 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    10801 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    10861 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    10921 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    10981 gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca
    11041 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    11101 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    11161 gggtttatta cagggacagc agagatccag tttggatcga taagcttgat atcgaattcg
    11221 gagcactgtc ctccgaacgt cggagcactg tcctccgaac gtcggagcac tgtcctccga
    11281 acgtcggagc actgtcctcc gaacggagca tgtcctccga acgtcggagc actgtcctcc
    11341 gaacgactag ttaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
    11401 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
    11461 cgatccagcc tctcgacatt cgttggatcg ccgccacc
//
