LOCUS       sequence-175259-        4283 bp    DNA     circular SYN 15-APR-2025
DEFINITION  mammalian expression of human p300 amino acids 1601-1900 with 
            N-terminal GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Askew EB, Bai S, Blackwelder AJ, Wilson EM
  TITLE     Transcriptional synergy between melanoma antigen gene protein-A11 
            (MAGE-11) and p300 in androgen receptor signaling.
  JOURNAL   J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 
            10.1074/jbc.M110.120600. Epub 2010 May 6.
  PUBMED    20448036
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.120600"; journalName: "J Biol Chem"; date: 
            "2010-07-9- 9"; volume: "285"; issue: "28"; pages: "21824-36"
COMMENT     Sequence Label: GAL-p300-1601-1900
FEATURES             Location/Qualifiers
     source          1..4283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             397..837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    1907..2041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1944..1963)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1998..2017
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2104..2123)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2388..2405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2559..3147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2639..2658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3318..4109,4110..4178))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3941..3960
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4179..4283)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacct tctgaggcgg aaagaaccag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt
      361 ggaggagatc taagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa
      421 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      481 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      541 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      601 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      661 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      721 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
      781 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg
      841 gaattcccgg ggatccgtcg acagattgct ggccctgctg ccaactccct gcctcccatt
      901 gttgatcctg atcctctcat cccctgcgat ctgatggatg gtcgggatgc gtttctcacg
      961 ctggcaaggg acaagcacct ggagttctct tcactccgaa gagcccagtg gtccaccatg
     1021 tgcatgctgg tggagctgca cacgcagagc caggaccgct ttgtctacac ctgcaatgaa
     1081 tgcaagcacc atgtggagac acgctggcac tgtactgtct gtgaggatta tgacttgtgt
     1141 atcacctgct ataacactaa aaaccatgac cacaaaatgg agaaactagg ccttggctta
     1201 gatgatgaga gcaacaacca gcaggctgca gccacccaga gcccaggcga ttctcgccgc
     1261 ctgagtatcc agcgctgcat ccagtctctg gtccatgctt gccagtgtcg gaatgccaat
     1321 tgctcactgc catcctgcca gaagatgaag cgggttgtgc agcataccaa gggttgcaaa
     1381 cggaaaacca atggcgggtg ccccatctgc aagcagctca ttgccctctg ctgctaccat
     1441 gccaagcact gccaggagaa caaatgcccg gtgccgttct gcctaaacat caagcagaag
     1501 ctccggcagc aacagctgca gcaccgacta cagcaggccc aaatgcttcg caggaggatg
     1561 gccagcatgc agcggactgg tgtggttggg cagcaacagg gcctcccttc ccccactcct
     1621 gccactccaa cgacaccaac tggccaacag ccaaccaccc cgcagacgcc ccagcccact
     1681 tctcagcctc agcctacccc tcccaatagc atgccaccct acttgcccag gactcaagct
     1741 gctggccctg tgtcccaggg taagtagatc gataatgcat ccgagctcta gataagtaat
     1801 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
     1861 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1921 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1981 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     2041 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     2101 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     2161 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     2221 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     2281 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg atatgcggtg tgaaataccg
     2341 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     2401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2461 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2521 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2581 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2641 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2761 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2821 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2881 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2941 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3061 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3121 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3361 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3421 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3481 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3541 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3601 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     3661 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3721 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3781 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3841 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3901 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     3961 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4021 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4081 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4141 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4261 aataaacaaa taggggttcc gcg
//
