LOCUS       sequence-175357-         960 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- 
            terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    386..507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(423..442)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     477..496
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(651..672)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     863..882
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgagctgtac aagtccggac tcagatcttc cccacccccg caggtctggc agctgatctc
       61 caaagtcttg gcccgccact tcagtgcggc agatgccagc agggtcctgg aacagctaca
      121 gagggattac gagaggtccc tgagtcgcct gacgctggac gacatcgaac ggttggccag
      181 ccgcttcctg caccctgaag tgactgagac aatggagaag ggcttctcca aataggtcga
      241 cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
      301 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
      361 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      421 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      481 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa
      541 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      601 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      661 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
      721 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
      781 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
      841 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
      901 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
//
