LOCUS       sequence-175398-        9769 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor template for generation of NGN3-ntdTomato reporter cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Otonkoski
  TITLE     Otonkoski lab Reporter cell lines plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro
FEATURES             Location/Qualifiers
     source          1..9769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             715..768
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             778..798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             808..828
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             835..2265
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    join(2287..2861,2862..2864,2865..2873)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2454..2471)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2681..2700
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2874..3473
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3554..3571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3560..3784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3831..3847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3855..3871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3924..3957
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4018..4514
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4053..4076)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4447..4466
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4554..5153
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     protein_bind    5710..5743
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6202..6221)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6203..6221)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6228..6245)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6228..6244)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6237..6259)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6385..6813
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6472..6491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6682..6703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7157..7951
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7211..7230)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7821..7840
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(7969..8037,8038..8829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8187..8206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9000..9588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9489..9508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9742..9759
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccccg gcctaagagc gagttggcac tgagcaagca gcgacggagt
      301 cggcgaaaga aggccaacga ccgcgagcgc aatcgaatgc acaacctcaa ctcggcactg
      361 gacgccctgc gcggtgtcct gcccaccttc ccagacgacg cgaagctcac caagatcgag
      421 acgctgcgct tcgcccacaa ctacatctgg gcgctgactc aaacgctgcg catagcggac
      481 cacagcttgt acgcgctgga gccgccggcg ccgcactgcg gggagctggg cagcccaggc
      541 ggttcccccg gggactgggg gtccctctac tccccagtct cccaggctgg cagcctgagt
      601 cccgccgcgt cgctggagga gcgacccggg ctgctggggg ccaccttttc cgcctgcttg
      661 agcccaggca gtctggcttt ctcagatttt ctcgctagcg tcgacggctc cggagagggc
      721 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat cgatggtcca
      781 aaaaagaaga gaaaggtatt cgatggtcca aaaaagaaga gaaaggtatt cgatatggtg
      841 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
      901 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      961 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1021 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     1081 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1141 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     1201 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     1261 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     1321 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     1381 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     1441 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     1501 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
     1561 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     1621 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1681 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     1741 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     1801 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1861 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     1921 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     1981 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     2041 gagatccacc aggccctgaa gctgaaggac ggcggccgct acctggtgga gttcaagacc
     2101 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     2161 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     2221 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaatttaa agaattccgc
     2281 cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     2341 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     2401 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     2461 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     2521 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     2581 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     2641 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     2701 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     2761 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     2821 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgaccg
     2881 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     2941 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     3001 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     3061 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     3121 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     3181 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     3241 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     3301 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     3361 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     3421 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     3481 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg aagatctaga
     3541 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3601 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3661 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3721 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3781 atggcttctg aggcggaaag aacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     3841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggcaattcc
     3901 gatcatattc aataaccctt aatataactt cgtataatgt atgctatacg aagttattag
     3961 gtctgaagag gagtttacgt ccagccaagc ttaggatctc gacctcgaaa ttctaccggg
     4021 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctggcac
     4081 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtagcgccaa
     4141 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     4201 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     4261 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     4321 agcggccaat agcagctttg ctccttcgct ttctgggctc agcagctggg aagggtgggt
     4381 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     4441 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     4501 ctccgggcct ttcgacctgc atccatctag atctcgagca gctgaagctt accatgaccg
     4561 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     4621 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     4681 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     4741 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     4801 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     4861 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     4921 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     4981 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5041 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5101 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     5161 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgatatcag
     5221 atccccggga tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa
     5281 gtttttcctg tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga
     5341 aggattggag ctacgggggt gggggtgggg tgggattaga taaatgcctg ctctttactg
     5401 aaggctcttt actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa
     5461 gcaaaaccaa attaagggcc agctcattcc tcccactcat gatctataga tctatagatc
     5521 tctcgtggga tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt
     5581 ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac
     5641 ttcattctca gtattgtttt gccaagttct aattccatca gaagctggtc gagatccgga
     5701 acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgaagagg
     5761 ttcactaggc gcgccgagct gtctgtcgct gtgctgtggg tgctaagggt aagggagagg
     5821 gagggagccg ggagccgtag agggtggccg acggcggcgg ccctcaaaag cacttgttcc
     5881 ttctgcttct ccctggctga cccctggccg gcccaggcct ccacgggggc ggcaggctgg
     5941 gttcattccc cggccctccg agccgcgcca acgcacgcaa cccttgctgc tgcccgcgcg
     6001 aagtgggcat tgcaaagtgc gctcatttta ggcctcctct ctgccaccac cccataatct
     6061 cattcaaaga atactagaat ggtagcacta cccggccgga gccgcccacc gtcttgggtc
     6121 gccctaccct cactcaagtc tgtctgcctc tcagtctctt accacccctc ctccaatgtg
     6181 atttctagag ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     6241 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6301 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6361 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6421 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6481 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6541 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6601 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6661 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6721 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6781 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aattcagggc gcaagggctg
     6841 ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa
     6901 tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc
     6961 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc
     7021 ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat
     7081 ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg
     7141 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     7201 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     7261 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7321 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7381 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     7441 cgaagtgccg gggcaggatc tcctgtcatc ccaccttgct cctgccgaga aagtatccat
     7501 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7561 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7621 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7681 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7741 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7801 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7861 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7921 cttctatcgc cttcttgacg agttcttctg aattgaaaaa ggaagagtat gagtattcaa
     7981 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8041 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8101 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8161 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8221 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8281 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8341 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8401 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8461 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8521 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8581 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8641 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8701 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8761 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8821 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8881 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8941 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
