LOCUS       sequence-175426-       10784 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Vector for reporter assay of one mouse super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl E
FEATURES             Location/Qualifiers
     source          1..10784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3276..3848
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3922..3931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3928..3930,3931..3933,3934..4647)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3928..3930,3931..3933,3934..4644)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3973..3994)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4234..4253)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4581..4602
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4692..4826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4716..4735)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4770..4789
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5496..5512)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5496..5512)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5549..5569)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5549..5567)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5588..5604)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5588..5604)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5601..5623)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5612..5628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5636..5642,5643..5660,5661..5666))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5681..5702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5819..5836)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5990..6578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6070..6089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6749..7540,7541..7609))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7372..7391
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7610..7714)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7740..8195)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7877..7898)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8089..8108
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8322..8344
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8336..8353
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8337..8353
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8363..8382
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8363..8381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctcc gtgtagggat taccatagtc
     1741 tgccaccaca tttggtttat atggagctgg agcgctaacc cagggttctg tgcctgctag
     1801 gtgttctacc aactgagcca aatccctagc cctgatggga ggaatggacg tgtctgcctg
     1861 gagatcagat tagaggacat ggtgacaacc aacacctcta gcactgtccc aatcatacac
     1921 ggtcctccag catttctggg aatggtgggg aatgtagcca ggcctcctcc tgaacttgac
     1981 agatgtcaga ggtggggtca agggctgacc tcagcaggtg gtaggtacag tcaggaagga
     2041 agcagaggac cagcagctcg ccctgggatc ctgggtggcc tcctctatct actatgttga
     2101 taggatgagt gtacaactga caaccccgtg agctggcagc aagtgccgct ggaggagcct
     2161 gcagaggaga gcctgttgag gactcaacag ggaggacaga ggaagtggtg ctgaggcctg
     2221 gagccaggag actttttaga gtcccagcca gcagggcccg cccaggcctg ttctggcctc
     2281 tggtttccag ccagtgggta attcagaaac tcctccagat tgctgctggg gcctgcttgc
     2341 cttgttcctc cttgctcaag ctttggcagc cacctcggat ctaccttcca gatgtgtgcc
     2401 cctgtattca tagctggtcc aaagtcagtc agtaccggga acctgaactg ggagttgggg
     2461 ggacaagcct ggaaaggatc caggttccat agaagagagg gagaggggcc ccaccctctg
     2521 ggtgctggga tgaaaacagt ggagacaggc tccagacctg tcttcagtcc agcctggagc
     2581 ctggaaggat ctcagaagcc agacagcaga aagtccgccc tcctggcaag ggctagccag
     2641 acagcagcca ggtcctcagg cagctccacc caatcttcaa cacccacctt ctgtccagaa
     2701 ggctgggaac ctggccttag tctcctcccc tccctcagca cctctcaagg tgggctgttg
     2761 atccccaccc tgttcaaatg caaattttct cagagcccag aaggaattga tactctgagg
     2821 ttcttagtcc tcacacttgt gaagaggttt ggggcccacc tgagttgggg catgagggac
     2881 acaccagggc tagatttggg ggagacaatc agggaaacag gcccagaaat gttacatagg
     2941 gtcaacagct aaataggtaa ctaccccagg caagacttcc tcagccatct ctcctgagct
     3001 ctccaggaaa aggcagattc cagctttggc acttaataat cacgggatgt tggacaagct
     3061 gtagctttcg cactgaaagg aggaaggact gaaacaaggg tcaccgcgtc ctgcctggct
     3121 ccccagaccc ggccttcttc catcacagcc attcctgtaa catagtagca accccagatc
     3181 tctcgactct agagggtata taatggatcc catcgcgtct cagcctcact ttgagctcct
     3241 ccacacgaat ttcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
     3301 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
     3361 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
     3421 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
     3481 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
     3541 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
     3601 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
     3661 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
     3721 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     3781 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
     3841 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
     3901 tcctcgacgg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     3961 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4021 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4081 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     4141 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4201 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4261 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4321 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4381 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4441 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4501 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     4561 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4621 ctcggcatgg acgagctgta caagtaaagc ggccgccacc gcggtggagc tcgaattaat
     4681 tcatcgatga tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     4741 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4801 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     4861 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     4921 ctgattatga tcctctagat cagatccgaa gatacggcca cgggtgctct tgatcctgtg
     4981 gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct
     5041 tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt
     5101 aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata
     5161 taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag
     5221 cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat
     5281 aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa
     5341 aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat
     5401 ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc
     5461 tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc
     5521 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     5581 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5641 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5701 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5761 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5821 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5881 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6301 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6421 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6541 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6601 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6661 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6721 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6781 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6841 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6901 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6961 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7021 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7081 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7141 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7201 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7261 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7321 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7381 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7441 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7501 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7561 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7621 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7681 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     7741 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7801 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7861 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7921 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     7981 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     8041 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8101 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     8161 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     8221 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8281 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     8341 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccaaata
     8401 gtaggaatta cccacctgta caagtgctga aaacttggat gaataagccc gtttgccatt
     8461 ttctactgct attttaaatc ttttctgtat ttgtctgcat ttgtctttac ccttgaataa
     8521 atgttttagt tgtttttttt caattatgac tgtgtttaac agacattatt acaataatat
     8581 gtaatagtag tacacactat tattatgtaa tagtacttgt tgactgtatt tgagaactgg
     8641 accgagtgag tgttacgtca cccattcaaa atgacttact tctggctcca acaaaatgaa
     8701 gttaattcag ttgccatttt tcactgtatg gacatcgccg tgttggagct agacgttgcc
     8761 aataagcaag aaagagccga gatgcgtcga gtctgagtca ccgttcctat ggcaacccct
     8821 ctaaccaatc agaagtaagc ttgttggaag tccacagcct accacttgaa agcgggctgc
     8881 acaaaatctg tcaaacgttt tgaacgttgg atgtgagagc acatactttt attaaggcat
     8941 ctggttggtc agtttataat atcaacaact tgggctacag aaaagaaaag ttattacaga
     9001 aattatgatt aacaagtaca tgttaaataa agattttaat atgaatgcca ccactggagc
     9061 attcatgcca tttggagctt cttcctgttt ggatcactag aaggaggagg tcactcatta
     9121 cagttctcat atacagtcgt tggttggttg gttggttggt agattgattg attgattgat
     9181 tgattgattg attgattgat tgattgattg attgattgat tgattgattg attgattggt
     9241 agtcaaaata agaaataatt tccacagatt cattacagaa atgattaaat gcatacataa
     9301 aaaactgggg ggggggggga tacaacaaca cacttaagtc acatttgcct acgtaaagaa
     9361 aagtaaagaa aatcaatagc tatattttac atctcctttt tttgctgtct tttaattagc
     9421 cttgttttgc tgttatcttg attgaaactg taacttcttc acctgcttct tttctttgta
     9481 ggttttgcca gcccagaaac aggcatggct gtgcaaggcc agagcaccat gcacaatgcg
     9541 ctgcatgtct tcatgaacgg ctcaatgtcc tcagtccagg gctcagccaa cgaccccatc
     9601 ttcctccttc accatgcttt cattgacagg taacaaacac gtcatgacat tagactgcac
     9661 agtttttgac aaagttcata caatctgttg tttatagctg ctacaattag tgaagtttgt
     9721 gaatgtactt ggatgagcag cgaaagatca attgagatca attgttagag tttggttgcc
     9781 ctgcagagca aagaacaaaa aataatctgg tggctttact gcgtgaggtt attattggtg
     9841 gaatagaaac acaaaacata attgcattta tttgtttaat tttttatctt atcttaactt
     9901 tcatcttgca tatttgtttc ttacatcatt tctagcatct ttgagcgctg gctaagaact
     9961 catcagcctc cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc
    10021 tactacatgg tgccattcct tcctctttat aggaatggag actacctcct gtccaacaat
    10081 gctcttggat acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg
    10141 ccctctgatc tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac
    10201 acaggtcaga ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt
    10261 actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct
    10321 ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat
    10381 taaggaagta aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc
    10441 tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt
    10501 taattatcaa actataagac agacaatcta atgccagtac acgctactca aagttgtaaa
    10561 acctcagatt taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc
    10621 ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg
    10681 gaagaggtgt attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact
    10741 ccctggtgtc tgaaacacag gccagatggg ccctcgagga tatc
//
