LOCUS       sequence-176271-        9746 bp    DNA     circular SYN 14-APR-2025
DEFINITION  3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-Auf1
FEATURES             Location/Qualifiers
     source          1..9746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      24..612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     513..532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     766..783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    900..921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(936..941,942..959,960..966)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    974..990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     979..1001
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     998..1014
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     998..1014
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1033..1053
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1035..1053
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1081..1307
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1308..1488
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1535..1660
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2157..2390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3913..3957
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4264..4467
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4418..4438
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4464..4488
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4698..4716
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4698..4715
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     4729..4748
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4807..4830
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      4940..4949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5920..6037
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6086..6596
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6515..6533
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6618..7217
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6618..6637)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7114..7134
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7345..7578
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7650..7771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7687..7706)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7741..7760
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7811..7946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7966..7985)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7967..7985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7995..8012)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7995..8011)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8004..8026)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8153..8608
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8240..8259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8450..8471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8634..8738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8739..8807,8808..9599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8957..8976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
       61 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      781 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      841 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      901 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      961 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1021 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1081 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     1141 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     1201 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     1261 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     1321 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     1381 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1441 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     1501 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     1561 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1621 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1681 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1741 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1801 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1861 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1921 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1981 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     2041 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     2101 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     2161 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     2221 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     2281 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     2341 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     2401 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     2461 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2521 aattacacaa gcttaatact gagagatccc ctcataattt ccccaaagcg taaccatgtg
     2581 tgaataaatt ttgagctagt agggttgcag ccacgagtaa gtcttccctt gttattgtgt
     2641 agccagaatg ccgcaaaact tccatgccta agcgaactgt tgagagtacg tttcgatttc
     2701 tgactgtgtt agcctggaag tgcttgtccc aaccttgttt ctgagcatga acgcccgcaa
     2761 gccaacatgt tagttgaagc atcagggcga ttagcagcat gatatcaaaa cgctctgagc
     2821 tgctcgttcg gctatggcgt aggcctagtc cgtaggcagg acttttcaag tctcggaagg
     2881 tttcttcaat ctgcattcgc ttcgaataga tattaacaag ttgtttgggt gttcgaattt
     2941 caacaggtaa gttagttgct agaatccatg gctcctttgc cgacgctgag tagattttag
     3001 gtgacgggtg gtgacaatga gtccgtgtcg agcgctgatt ttttcggcct ttagagcgag
     3061 atttatacaa tagaatttgg catgagattg gattgctttt agtcagcctc ttatagccta
     3121 aagtctttga gtgactagat gacatatcat gtaagttgct gataggtttc cagttttccg
     3181 ctcctaggtc tgcatattgt acttttcctc ttactcgact taaccagtac caacccagct
     3241 tctcaacgga tttataccat ggcactttaa agccagcatc actgacaatg agcggtgtgg
     3301 tgttactcgg tagaatgctc gcaaggtcgg ctagaaattg gtcatgagct ttctttgaac
     3361 attgctctga aagcgggaac gctttctcat aaagagtaac agaacgaccg tgtagtgcga
     3421 ctgaagctcg caataccata agccgttttt gctcacggat atcagaccag tcaacaagta
     3481 caatgggcat cgtattgccc gaacagataa agctagcatg ccaacggtat acagcgagtc
     3541 gctctttgtg gaggtgacga ttacctaaca atcggtcgat tcgtttgatg ttatgttttg
     3601 ttctcgcttt ggttggcagg ttacggccaa gttcggtaag agtgagagtt ttacagtcaa
     3661 gtaaggcgtg gcaagccaac gttaagctgt tgagtcgttt taagtgtaat tcggggcaga
     3721 attggtaaag agagtcgtgt aaaatatcga gttcgcacat tttgttgtct gattattgat
     3781 ttttggcgaa accatttgat catatgacaa gatgtgtatc taccttaact taatgatttt
     3841 gataaaaatc attaggggat tcatcagagc ttaatacact ccttaattga agaatcgcaa
     3901 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     3961 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     4021 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     4081 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc ccatatgcca
     4141 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     4201 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4261 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4321 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4381 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4441 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     4501 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
     4561 gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga
     4621 gtctataggc ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta
     4681 tgtatcatac acatacgatt taggtgacac tatagaataa catccacttt gcctttctct
     4741 ccacaggtgt ccactcccag gtccaactgc acctcggttc tatcgattga attcaccatg
     4801 gaaatggact acaaggatga cgatgacaag ggtggtggtg gtggtgcgtc gacgatgtcg
     4861 gaggagcagt tcggcgggga cggggcggcg gcagcggcaa cggcggcggt aggcggctcg
     4921 gcgggcgagc aggagggagc catggtggcg gcgacacagg gggcagcggc ggcggcggga
     4981 agcggagccg ggaccggggg cggaaccgcg tctggaggca ccgaaggggg cagcgccgag
     5041 tcggaggggg cgaagattga cgccagtaag aacgaggagg atgaagggaa aatgtttata
     5101 ggaggcctta gctgggacac tacaaagaaa gatctgaagg actacttttc caaatttggt
     5161 gaagttgtag actgcactct gaagttagat cctatcacag ggcgatcaag gggttttggc
     5221 tttgtgctat ttaaagaatc ggagagtgta gataaggtca tggatcaaaa agaacataaa
     5281 ttgaatggga aggtgattga tcctaaaagg gccaaagcca tgaaaacaaa agagccggtt
     5341 aaaaaaattt ttgttggtgg cctttctcca gatacacctg aagagaaaat aagggagtac
     5401 tttggtggtt ttggtgaggt ggaatccata gagctcccca tggacaacaa gaccaataag
     5461 aggcgtgggt tctgctttat tacctttaag gaagaagaac cagtgaagaa gataatggaa
     5521 aagaaatacc acaatgttgg tcttagtaaa tgtgaaataa aagtagccat gtcgaaggaa
     5581 caatatcagc aacagcaaca gtggggatct agaggaggat ttgcaggaag agctcgtgga
     5641 agaggtggtg gccccagtca aaactggaac cagggatata gtaactattg gaatcaaggc
     5701 tatggcaact atggatataa cagccaaggt tacggtggtt atggaggata tgactacact
     5761 ggttacaaca actactatgg atatggtgat tatagcaacc agcagagtgg ttatgggaag
     5821 gtatccaggc gaggtggtca tcaaaatagc tacaaaccat actaagcggc cgcagtgaga
     5881 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     5941 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     6001 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     6061 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
     6121 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     6181 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     6241 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     6301 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     6361 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     6421 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     6481 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     6541 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     6601 atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6661 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6721 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6781 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6841 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6901 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6961 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7021 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7081 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7141 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7201 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     7261 gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7321 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7381 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7441 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7501 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7561 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7621 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7681 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7741 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7801 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7861 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7921 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7981 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8041 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8101 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     8161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8581 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8641 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8701 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8761 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8821 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8881 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8941 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9001 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9061 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9121 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9181 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9241 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9301 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9361 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9421 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9481 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9541 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9601 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9661 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9721 tttcgttcca ctgagcgtca gacccc
//
